{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:54:19Z","timestamp":1791244459498,"version":"4.1.0"},"reference-count":54,"publisher":"Radiation Research Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2025,6,19]]},"abstract":"<jats:p>Hair is an attractive sample for determining exposure to ionizing radiation due to its non-invasive nature. A biological tissue comprised mainly of keratin protein, hair is susceptible to oxidative or reductive stress by direct or indirect damage mechanisms. In this report, changes observed in the Raman spectra associated with hair protein from ionizing radiation were assessed for biodosimetry. Raman spectra were obtained from the hairs of a mixed sex cohort of irradiated C57BL\/6 mice (N = 32 total) with doses of gamma rays ranging from 0\u20134 Gy. Radiation-dependent changes in the Raman spectra of the hairs provided molecular-specific signals that can inform about the damage mechanism. Partial least-squares discriminant analysis (PLS-DA) models incorporating automated variable selection for each sex showed classification of controls or exposed at 80% accuracy based on cross-validation. Models show only slight differences in performance based on the mouse's sex from which the sample originated. This slight difference is consistent with PLS-DA models that show marginal cross-validation sensitivity (\u223c60%) in predicting the sex of the mouse from the Raman hair spectrum. Utilizing PLS regression, a dose-response model including both sexes showed root-mean-squared error (RMSE) 61 Gy. The ability to determine dose or exposure from plucked hair with Raman spectroscopy would provide a needed tool for rapid medical triage after unexpected exposure.<\/jats:p>","DOI":"10.1667\/rade-25-00046.1","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T05:14:25Z","timestamp":1750310065000},"page":"197-211","source":"Crossref","is-referenced-by-count":1,"title":["Raman Spectroscopic Biodosimetry Using Protein in Murine Hair"],"prefix":"10.1667","volume":"204","author":[{"given":"Spencer A.","family":"Witte","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Poonia","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallard","family":"Woodward","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanchun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marshleen","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naduparambil K.","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary D.","family":"Schultz","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, The Ohio State University, Columbus, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"6","doi-asserted-by":"publisher","unstructured":"WL Santivasi  and F Xia  \"Antioxid Redox Signal.\"  21, \"Ionizing radiation-induced DNA damage, response, and repair.\" 251 (2014)","DOI":"10.1089\/ars.2013.5668"},{"key":"20","doi-asserted-by":"publisher","unstructured":"GP Sharma, A Frei, B Fish, et al.  \"Front Physiol.\"  14, \"Biological sex differences in renin angiotensin system enzymes ACE and ACE2 regulate normal tissue response to radiation injury.\" 1191237 (2023)","DOI":"10.3389\/fphys.2023.1191237"},{"key":"1","unstructured":"\"Acute Radiation Syndrome: Information for Clinicians.\""},{"key":"5","doi-asserted-by":"publisher","unstructured":"JA Reisz, N Bansal, J Qian, et al.  \"Antioxid Redox Signal.\"  21, \"Effects of ionizing radiation on biological molecules\u2014Mechanisms of damage and emerging methods of detection.\" 260 (2014)","DOI":"10.1089\/ars.2013.5489"},{"key":"7","doi-asserted-by":"publisher","unstructured":"JF. 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