{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:31:48Z","timestamp":1793421108759,"version":"4.5.0"},"reference-count":63,"publisher":"Radiation Research Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2026,7,27]]},"abstract":"<jats:p>In addition to immediate cell lethality, radiation exposure has long-term effects on the immune system, particularly T lymphocytes, causing persistent functional abnormalities post-exposure. Uncertainties remain regarding the long-lasting consequences on T cell immunity in survivors of the acute radiation syndrome and the underlying mechanisms of those consequences. Here, we investigated delayed effects of acute radiation exposure on CD8+ T cell immunity using the Listeria monocytogenes infection model. Impaired CD8+ T cell activation, reduced terminal effector formation upon infection, and exacerbated listeriosis were found in mice at 4 weeks postirradiation with sublethal doses. To elucidate how radiation affects the functionality of CD8+ T cells at various differentiation stages, we performed single-cell transcriptomic profiling. Radiation altered cluster distribution pre-infection and impaired terminal effector and memory precursor effector formation post-infection. Differential gene analysis highlighted radiation-induced gene expression changes, including cytokines and mitochondrial proteins, in a cluster-specific manner post-infection. The study demonstrates dysfunction of newly replenished na\u00efve T cells, which results in impaired CD8+ T cell immunity after irradiation.<\/jats:p>","DOI":"10.1667\/rade-25-00087.1","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:37:56Z","timestamp":1785497876000},"page":"394-408","source":"Crossref","is-referenced-by-count":0,"title":["Radiation Induces Persistent Dysregulation and Functional Impairment in CD8+ T Cell Immunity"],"prefix":"10.1667","volume":"206","author":[{"given":"Anika","family":"Kot","sequence":"first","affiliation":[{"name":"Department of Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorreta Yun-Tien","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhinuo","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert J.","family":"Fornace","sequence":"additional","affiliation":[{"name":"Department of Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng-Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"J Fern\u00e1ndez-Garc\u00eda, F Franco, S Parik, et al.  \"Cell Rep.\"  41, \"CD8+ T cell metabolic rewiring defined by scRNA-seq identifies a critical role of ASNS expression dynamics in T cell differentiation.\" 111639 (2022)","DOI":"10.1016\/j.celrep.2022.111639"},{"key":"2","doi-asserted-by":"crossref","unstructured":"GJ van der Windt, D O'Sullivan, B Everts, et al.  \"Proc Natl Acad Sci U S A.\"  110, \"CD8 memory T cells have a bioenergetic advantage that underlies their rapid recall ability.\" 14336 (2013)","DOI":"10.1073\/pnas.1221740110"},{"key":"3","doi-asserted-by":"crossref","unstructured":"K Murali-Krishna, JD Altman, M Suresh, et al.  \"Immunity.\"  8, \"Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection.\" 177 (1998)","DOI":"10.1016\/S1074-7613(00)80470-7"},{"key":"4","doi-asserted-by":"crossref","unstructured":"R Ahmed  and D Gray  \"Science.\"  272, \"Immunological memory and protective immunity: understanding their relation.\" 54 (1996)","DOI":"10.1126\/science.272.5258.54"},{"key":"5","doi-asserted-by":"crossref","unstructured":"CJ Fox, PS Hammerman, CB Thompson,  \"Nat Rev Immunol.\"  5, \"Fuel feeds function: energy metabolism and the T-cell response.\" 844 (2005)","DOI":"10.1038\/nri1710"},{"key":"6","doi-asserted-by":"crossref","unstructured":"GJ van der Windt  and EL Pearce  \"Immunol Rev.\"  249, \"Metabolic switching and fuel choice during T-cell differentiation and memory development.\" 27 (2012)","DOI":"10.1111\/j.1600-065X.2012.01150.x"},{"key":"7","doi-asserted-by":"crossref","unstructured":"KA Frauwirth  and CB Thompson  \"J Immunol.\"  172, \"Regulation of T lymphocyte metabolism.\" 4661 (2004)","DOI":"10.4049\/jimmunol.172.8.4661"},{"key":"8","doi-asserted-by":"crossref","unstructured":"R Wang, CP Dillon, LZ Shi, et al.  \"Immunity.\"  35, \"The transcription factor Myc controls metabolic reprogramming upon T lymphocyte activation.\" 871 (2011)","DOI":"10.1016\/j.immuni.2011.09.021"},{"key":"9","doi-asserted-by":"crossref","unstructured":"EA Newsholme, B Crabtree, MS Ardawi,  \"Q J Exp Physiol.\"  70, \"Glutamine metabolism in lymphocytes: its biochemical, physiological and clinical importance.\" 473 (1985)","DOI":"10.1113\/expphysiol.1985.sp002935"},{"key":"10","doi-asserted-by":"crossref","unstructured":"EA Newsholme, B Crabtree, MS Ardawi,  \"Biosci Rep.\"  5, \"The role of high rates of glycolysis and glutamine utilization in rapidly dividing cells.\" 393 (1985)","DOI":"10.1007\/BF01116556"},{"key":"11","doi-asserted-by":"crossref","unstructured":"IR. Radford,  \"Int J Radiat Biol.\"  65, \"Radiation response of mouse lymphoid and myeloid cell lines. Part I. Sensitivity to killing by ionizing radiation, rate of loss of viability, and cell type of origin.\" 203 (1994)","DOI":"10.1080\/09553009414550241"},{"key":"12","doi-asserted-by":"crossref","unstructured":"ST Palayoor, RM Macklis, EA Bump, et al.  \"Radiat Res.\"  141, \"Modulation of radiation-induced apoptosis and G2\/M block in murine T-lymphoma cells.\" 235 (1995)","DOI":"10.2307\/3579000"},{"key":"13","doi-asserted-by":"crossref","unstructured":"TT Sheu  and BL Chiang  \"Int J Mol Sci.\"  22, \"Lymphopenia, lymphopenia-induced proliferation, and autoimmunity.\" 4152 (2021)","DOI":"10.3390\/ijms22084152"},{"key":"14","doi-asserted-by":"crossref","unstructured":"L Cella, S Monti, R Pacelli, et al.  \"Radiother Oncol.\"  190, \"Modeling frameworks for radiation induced lymphopenia: A critical review.\" 110041 (2024)","DOI":"10.1016\/j.radonc.2023.110041"},{"key":"15","doi-asserted-by":"crossref","unstructured":"C Cozzarini, B Noris Chiorda, C Sini, et al.  \"Int J Radiat Oncol Biol Phys.\"  95, \"Hematologic toxicity in patients treated with postprostatectomy whole-pelvis irradiation with different intensity modulated radiation therapy techniques Is not negligible and is prolonged: Preliminary results of a longitudinal, observational study.\" 690 (2016)","DOI":"10.1016\/j.ijrobp.2016.01.022"},{"key":"16","doi-asserted-by":"crossref","unstructured":"TN Pham, J Coupey, SM Candeias, et al.  \"J Exp Clin Cancer Res.\"  42, \"Beyond lymphopenia, unraveling radiation-induced leucocyte subpopulation kinetics and mechanisms through modeling approaches.\" 50 (2023)","DOI":"10.1186\/s13046-023-02621-4"},{"key":"17","doi-asserted-by":"crossref","unstructured":"JT White, EW Cross, MA Burchill, et al.  \"Nat Commun.\"  7, \"Virtual memory T cells develop and mediate bystander protective immunity in an IL-15-dependent manner.\" 11291 (2016)","DOI":"10.1038\/ncomms11291"},{"key":"18","doi-asserted-by":"crossref","unstructured":"JY Lee, SE Hamilton, AD Akue, et al.  \"Proc Natl Acad Sci U S A.\"  110, \"Virtual memory CD8 T cells display unique functional properties.\" 13498 (2013)","DOI":"10.1073\/pnas.1307572110"},{"key":"21","doi-asserted-by":"crossref","unstructured":"M Heidarian, IJ Jensen, SK Kannan, et al.  \"Proc Natl Acad Sci U S A.\"  120, \"Sublethal whole-body irradiation induces permanent loss and dysfunction in pathogen-specific circulating memory CD8 T cell populations.\" e2302785120 (2023)","DOI":"10.1073\/pnas.2302785120"},{"key":"22","doi-asserted-by":"crossref","unstructured":"B Pasche, S Kalaydjiev, TJ Franz, et al.  \"Infect Immun.\"  73, \"Sex-dependent susceptibility to Listeria monocytogenes infection is mediated by differential interleukin-10 production.\" 5952 (2005)","DOI":"10.1128\/IAI.73.9.5952-5960.2005"},{"key":"25","doi-asserted-by":"crossref","unstructured":"E Shaulian  and M Karin  \"Nat Cell Biol.\"  4, \"AP-1 as a regulator of cell life and death.\" E131 (2002)","DOI":"10.1038\/ncb0502-e131"},{"key":"26","doi-asserted-by":"crossref","unstructured":"S Nishad  and A Ghosh  \"Radiat Environ Biophys.\"  55, \"Gene expression of immediate early genes of AP-1 transcription factor in human peripheral blood mononuclear cells in response to ionizing radiation.\" 431 (2016)","DOI":"10.1007\/s00411-016-0662-5"},{"key":"27","doi-asserted-by":"crossref","unstructured":"JA Herring, WS Elison, JS Tessem,  \"Cells.\"  8, \"Function of Nr4a orphan nuclear receptors in proliferation, apoptosis and fuel utilization across tissues.\" 1373 (2019)","DOI":"10.3390\/cells8111373"},{"key":"28","doi-asserted-by":"crossref","unstructured":"T MacVicar  and T Langer  \"J Cell Sci.\"  129, \"OPA1 processing in cell death and disease - the long and short of it.\" 2297 (2016)","DOI":"10.1242\/jcs.159186"},{"key":"29","doi-asserted-by":"crossref","unstructured":"OC Los\u00f3n, Z Song, H Chen, et al.  \"Mol Biol Cell.\"  24, \"Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission.\" 659 (2013)","DOI":"10.1091\/mbc.e12-10-0721"},{"key":"30","doi-asserted-by":"crossref","unstructured":"S Bahrami  and F Drabl\u00f8s  \"Adv Biol Regul.\"  62, \"Gene regulation in the immediate-early response process.\" 37 (2016)","DOI":"10.1016\/j.jbior.2016.05.001"},{"key":"31","doi-asserted-by":"crossref","unstructured":"RR Weichselbaum, D Hallahan, Z Fuks, et al.  \"Int J Radiat Oncol Biol Phys.\"  30, \"Radiation induction of immediate early genes: effectors of the radiation-stress response.\" 229 (1994)","DOI":"10.1016\/0360-3016(94)90539-8"},{"key":"32","doi-asserted-by":"crossref","unstructured":"T Ueda, Y Kohama, H Sakurai,  \"Cell Signal.\"  55, \"IER family proteins are regulators of protein phosphatase PP2A and modulate the phosphorylation status of CDC25A.\" 81 (2019)","DOI":"10.1016\/j.cellsig.2018.12.012"},{"key":"33","doi-asserted-by":"crossref","unstructured":"SE Syafruddin, MA Mohtar, WF Wan Mohamad Nazarie, et al.  \"Biomolecules.\"  10, \"Two sides of the same coin: The roles of KLF6 in physiology and pathophysiology.\" 1378 (2020)","DOI":"10.3390\/biom10101378"},{"key":"34","doi-asserted-by":"crossref","unstructured":"PK Jani, G Petkau, Y Kawano, et al.  \"PLoS Biol.\"  21, \"The miR-221\/222 cluster regulates hematopoietic stem cell quiescence and multipotency by suppressing both Fos\/AP-1\/IEG pathway activation and stress-like differentiation to granulocytes.\" e3002015 (2023)","DOI":"10.1371\/journal.pbio.3002015"},{"key":"35","doi-asserted-by":"crossref","unstructured":"V Andreoli, RC Gehrau, JL Bocco,  \"IUBMB Life.\"  62, \"Biology of Kr\u00fcppel-like factor 6 transcriptional regulator in cell life and death.\" 896 (2010)","DOI":"10.1002\/iub.396"},{"key":"36","doi-asserted-by":"crossref","unstructured":"ME Sabatino, A Castellaro, AC Racca, et al.  \"Front Cell Dev Biol.\"  7, \"Kr\u00fcppel-like factor 6 Is required for oxidative and oncogene-induced cellular senescence.\" 297 (2019)","DOI":"10.3389\/fcell.2019.00297"},{"key":"37","doi-asserted-by":"crossref","unstructured":"C Hu, J Yang, Z Qi, et al.  \"MedComm (2020).\"  3, \"Heat shock proteins: Biological functions, pathological roles, and therapeutic opportunities.\" e161 (2022)","DOI":"10.1002\/mco2.161"},{"key":"38","doi-asserted-by":"crossref","unstructured":"E Schmitt, M Gehrmann, M Brunet, et al.  \"J Leukoc Biol.\"  81, \"Intracellular and extracellular functions of heat shock proteins: repercussions in cancer therapy.\" 15 (2007)","DOI":"10.1189\/jlb.0306167"},{"key":"39","doi-asserted-by":"crossref","unstructured":"D Lanneau, M Brunet, E Frisan, et al.  \"J Cell Mol Med.\"  12, \"Heat shock proteins: essential proteins for apoptosis regulation.\" 743 (2008)","DOI":"10.1111\/j.1582-4934.2008.00273.x"},{"key":"40","doi-asserted-by":"crossref","unstructured":"V Magg, A Manetto, K Kopp, et al.  \"Cell Rep.\"  43, \"Turnover of PPP1R15A mRNA encoding GADD34 controls responsiveness and adaptation to cellular stress.\" 114069 (2024)","DOI":"10.1016\/j.celrep.2024.114069"},{"key":"42","doi-asserted-by":"crossref","unstructured":"Y Bai, M Hu, Z Chen, et al.  \"Front Immunol.\"  12, \"Single-cell transcriptome analysis reveals RGS1 as a new marker and promoting factor for T-cell exhaustion in multiple cancers.\" 767070 (2021)","DOI":"10.3389\/fimmu.2021.767070"},{"key":"44","doi-asserted-by":"crossref","unstructured":"S Yamazaki, T Muta, K Takeshige,  \"J Biol Chem.\"  276, \"A novel IkappaB protein, IkappaB-zeta, induced by proinflammatory stimuli, negatively regulates nuclear factor-kappaB in the nuclei.\" 27657 (2001)","DOI":"10.1074\/jbc.M103426200"},{"key":"45","doi-asserted-by":"crossref","unstructured":"MA Daniels, D Luera, E Teixeiro,  \"Front Immunol.\"  14, \"NF\u03baB signaling in T cell memory.\" 1129191 (2023)","DOI":"10.3389\/fimmu.2023.1129191"},{"key":"46","doi-asserted-by":"crossref","unstructured":"Y Xia, S Shen, IM Verma,  \"Cancer Immunol Res.\"  2, \"NF-\u03baB, an active player in human cancers.\" 823 (2014)","DOI":"10.1158\/2326-6066.CIR-14-0112"},{"key":"48","doi-asserted-by":"crossref","unstructured":"L Catrysse, L Vereecke, R Beyaert, et al.  \"Trends Immunol.\"  35, \"A20 in inflammation and autoimmunity.\" 22 (2014)","DOI":"10.1016\/j.it.2013.10.005"},{"key":"49","doi-asserted-by":"crossref","unstructured":"M Giordano, R Roncagalli, P Bourdely, et al.  \"Proc Natl Acad Sci U S A.\"  111, \"The tumor necrosis factor alpha-induced protein 3 (TNFAIP3, A20) imposes a brake on antitumor activity of CD8 T cells.\" 11115 (2014)","DOI":"10.1073\/pnas.1406259111"},{"key":"50","doi-asserted-by":"crossref","unstructured":"M Hammer, J Mages, H Dietrich, et al.  \"J Exp Med.\"  203, \"Dual specificity phosphatase 1 (DUSP1) regulates a subset of LPS-induced genes and protects mice from lethal endotoxin shock.\" 15 (2006)","DOI":"10.1084\/jem.20051753"},{"key":"51","doi-asserted-by":"crossref","unstructured":"Y Zhang, JM Reynolds, SH Chang, et al.  \"J Biol Chem.\"  284, \"MKP-1 is necessary for T cell activation and function.\" 30815 (2009)","DOI":"10.1074\/jbc.M109.052472"},{"key":"52","doi-asserted-by":"crossref","unstructured":"H Seo, YC Cho, A Ju, et al.  \"Sci Rep.\"  7, \"Dual-specificity phosphatase 5 acts as an anti-inflammatory regulator by inhibiting the ERK and NF-\u03baB signaling pathways.\" 17348 (2017)","DOI":"10.1038\/s41598-017-17591-9"},{"key":"53","doi-asserted-by":"crossref","unstructured":"P Chen, J Li, J Barnes, et al.  \"J Immunol.\"  169, \"Restraint of proinflammatory cytokine biosynthesis by mitogen-activated protein kinase phosphatase-1 in lipopolysaccharide-stimulated macrophages.\" 6408 (2002)","DOI":"10.4049\/jimmunol.169.11.6408"},{"key":"54","doi-asserted-by":"crossref","unstructured":"C Tiedje, MD Diaz-Mu\u00f1oz, P Trulley, et al.  \"Nucleic Acids Res.\"  44, \"The RNA-binding protein TTP is a global post-transcriptional regulator of feedback control in inflammation.\" 7418 (2016)","DOI":"10.1093\/nar\/gkw474"},{"key":"55","doi-asserted-by":"crossref","unstructured":"R Canzoneri, J Naipauer, M Stedile, et al.  \"J Mammary Gland Biol Neoplasia.\"  25, \"Identification of an AP1-ZFP36 regulatory network associated with breast cancer prognosis.\" 163 (2020)","DOI":"10.1007\/s10911-020-09448-1"},{"key":"56","doi-asserted-by":"crossref","unstructured":"M Liebmann, S Hucke, K Koch, et al.  \"Proc Natl Acad Sci U S A.\"  115, \"Nur77 serves as a molecular brake of the metabolic switch during T cell activation to restrict autoimmunity.\" E8017 (2018)","DOI":"10.1073\/pnas.1721049115"},{"key":"57","doi-asserted-by":"crossref","unstructured":"X Liu, Y Wang, H Lu, et al.  \"Nature.\"  567, \"Genome-wide analysis identifies NR4A1 as a key mediator of T cell dysfunction.\" 525 (2019)","DOI":"10.1038\/s41586-019-0979-8"},{"key":"58","doi-asserted-by":"crossref","unstructured":"L Odagiu, J May, S Boulet, et al.  \"Front Endocrinol (Lausanne).\"  11, \"Role of the orphan nuclear receptor NR4A family in T-cell biology.\" 624122 (2020)","DOI":"10.3389\/fendo.2020.624122"},{"key":"59","doi-asserted-by":"crossref","unstructured":"AM Amitrano, BJ Berry, K Lim, et al.  \"Front Immunol.\"  12, \"Optical control of CD8+ T cell metabolism and effector functions.\" 666231 (2021)","DOI":"10.3389\/fimmu.2021.666231"},{"key":"60","doi-asserted-by":"crossref","unstructured":"L Almeida, M Lochner, L Berod, et al.  \"Semin Immunol.\"  28, \"Metabolic pathways in T cell activation and lineage differentiation.\" 514 (2016)","DOI":"10.1016\/j.smim.2016.10.009"},{"key":"61","doi-asserted-by":"crossref","unstructured":"X Zhai, K Liu, H Fang, et al.  \"Sci Adv.\"  7, \"Mitochondrial C1qbp promotes differentiation of effector CD8+ T cells via metabolic-epigenetic reprogramming.\" eabk0490 (2021)","DOI":"10.1126\/sciadv.abk0490"},{"key":"62","doi-asserted-by":"crossref","unstructured":"ME Cook, TR Bradstreet, AM Webber, et al.  \"Sci Immunol.\"  7, \"The ZFP36 family of RNA binding proteins regulates homeostatic and autoreactive T cell responses.\" eabo0981 (2022)","DOI":"10.1126\/sciimmunol.abo0981"},{"key":"63","doi-asserted-by":"crossref","unstructured":"MJ Moore, NE Blachere, JJ Fak, et al.  \"Elife.\"  7, \"ZFP36 RNA-binding proteins restrain T cell activation and anti-viral immunity.\" e33057 (2018)","DOI":"10.7554\/eLife.33057"},{"key":"23","doi-asserted-by":"crossref","unstructured":"LS Levine, KJ Hiam-Galvez, DM Marquez, et al.  \"Immunity.\"  54, \"Single-cell analysis by mass cytometry reveals metabolic states of early-activated CD8+ T cells during the primary immune response.\" 829 (2021)","DOI":"10.1016\/j.immuni.2021.02.018"},{"key":"24","doi-asserted-by":"crossref","unstructured":"HK Chung, B McDonald, SM Kaech,  \"J Exp Med.\"  218, \"The architectural design of CD8+ T cell responses in acute and chronic infection: Parallel structures with divergent fates.\" e20201730 (2021)","DOI":"10.1084\/jem.20201730"},{"key":"19","doi-asserted-by":"crossref","unstructured":"HH Li, YW Wang, R Chen, et al.  \"Int J Biol Sci.\"  11, \"Ionizing radiation impairs T cell activation by affecting metabolic reprogramming.\" 726 (2015)","DOI":"10.7150\/ijbs.12009"},{"key":"47","doi-asserted-by":"crossref","unstructured":"C Betlazar, RJ Middleton, RB Banati, et al.  \"Redox Biol.\"  9, \"The impact of high and low dose ionising radiation on the central nervous system.\" 144 (2016)","DOI":"10.1016\/j.redox.2016.08.002"},{"key":"41","doi-asserted-by":"crossref","unstructured":"I Novoa, H Zeng, HP Harding, et al.  \"J Cell Biol.\"  153, \"Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2alpha.\" 1011 (2001)","DOI":"10.1083\/jcb.153.5.1011"},{"key":"43","doi-asserted-by":"crossref","unstructured":"T Liu, L Zhang, D Joo, et al.  \"Signal Transduct Target Ther.\"  2, \"Nf-\u03bab signaling in inflammation.\" 17023 (2017)","DOI":"10.1038\/sigtrans.2017.23"},{"key":"20","doi-asserted-by":"crossref","unstructured":"D Li, R Chen, YW Wang, et al.  \"Int J Radiat Biol.\"  94, \"Prior irradiation results in elevated programmed cell death protein 1 (PD-1) in T cells.\" 488 (2018)","DOI":"10.1080\/09553002.2017.1400192"}],"container-title":["Radiation Research"],"original-title":[],"link":[{"URL":"https:\/\/radiation-research.kglmeridian.com\/view\/journals\/rare\/aop\/article-10.1667-RADE-25-00087.1\/article-10.1667-RADE-25-00087.1.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/radiation-research.kglmeridian.com\/downloadpdf\/journals\/rare\/aop\/article-10.1667-RADE-25-00087.1\/article-10.1667-RADE-25-00087.1.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:14:57Z","timestamp":1793420097000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/radiation-research\/volume-206\/issue-4\/RADE-25-00087.1\/Radiation-Induces-Persistent-Dysregulation-and-Functional-Impairment-in-CD8-T\/10.1667\/RADE-25-00087.1.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,27]]},"references-count":63,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,10,1]]}},"URL":"https:\/\/doi.org\/10.1667\/rade-25-00087.1","relation":{},"ISSN":["0033-7587","1938-5404"],"issn-type":[{"value":"0033-7587","type":"print"},{"value":"1938-5404","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,27]]}}}