{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:00:52Z","timestamp":1791237652836,"version":"4.1.0"},"reference-count":32,"publisher":"Radiation Research Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2025,12,12]]},"abstract":"<jats:p>Conventionally, the microparticles that are derived from the platelet surface membrane in the size range of 0.1 to 1.0 \u00b5m in diameter, as detected by flow cytometry with platelet-specific marker\/s, are collectively categorized as PMPs, despite their structural heterogeneity. We aim to determine the detailed structural classification of this heterogeneous population based on their scatter properties using flow cytometry, both before and several months after total-body irradiation (TBI), an exposure that may occur during nuclear attacks or accidents, or as part of myeloablative conditioning prior to allogeneic stem cell transplantation for leukemia, in wild-type C57BL\/6J (WT) mice and in a relatively radiation-sensitive strain deficient in platelet glycoprotein (GP) Ib\u03b1 (KO), including both sexes and with or without agonist treatments. We exposed WT and KO mice to a single dose of TBI. We collected blood 233 days after 7.8 Gy from male mice and 254 days after 8.0 Gy from female mice, prepared platelet-rich plasma (PRP), activated PRP with 3 different platelet agonists [adenosine 5\u2032-diphosphate (ADP), collagen and thrombin], and characterized the structural heterogeneity of PMPs. Additionally, we quantified the total number of microparticles using Nanoparticle Tracking Analysis (NTA) in WT and KO mice of both sexes. We then cocultured either RAW264.7 cells or splenic CD191 B cells with microparticles from sham-irradiated and irradiated female mice of both strains to measure reactive oxygen species (ROS) generation and immunoglobulin production, respectively, to assess strain- and TBI-dependent effects of PMPs on immune cell function. We identified 3 distinct PMP subpopulations based on flow cytometry scatter profiles. The percentage of PMP subpopulations altered depending on irradiation status, GPIb\u03b1 expression, platelet agonist used, and biological sex. Depending on sex, mouse strain, and TBI status, total microparticle count and microparticles-mediated ROS generation and immunoglobin production were altered in RAW264.7 cells and splenic B cells, respectively. These findings offer important insights into a poorly understood area of platelet biology and highlight the potential significance of PMP subpopulations in the context of radiation exposure.<\/jats:p>","DOI":"10.1667\/rade-25-00165.1","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T22:09:35Z","timestamp":1766182175000},"page":"222-233","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Characterization of Platelet Surface-membrane-derived Microparticles before and after Total-body Irradiation"],"prefix":"10.1667","volume":"205","author":[{"given":"Bernice","family":"Nounamo","sequence":"first","affiliation":[{"name":"Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravi P.","family":"Cholia","sequence":"additional","affiliation":[{"name":"Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruofei","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Biostatistics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enoch K.","family":"Larrey","sequence":"additional","affiliation":[{"name":"Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith Nathalie","family":"Pineda","sequence":"additional","affiliation":[{"name":"Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Porter","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry","family":"Ware","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rupak","family":"Pathak","sequence":"additional","affiliation":[{"name":"Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"N Kuriyama, Y Nagakane, A Hosomi, et al.  \"Clinical and Applied Thrombosis\/Hemostasis: official Journal of The international Academy of Clinical and Applied Thrombosis\/Hemostasis.\"  16, \"Evaluation of Factors Associated with Elevated Levels of Platelet-Derived Microparticles in The Acute Phase of Cerebral infarction.\" 26 (2010)","DOI":"10.1177\/1076029609338047"},{"key":"2","doi-asserted-by":"crossref","unstructured":"Knijff-Dutmer Eaj, J Koerts, R Nieuwland, et al.  \"Arthritis and Rheumatism.\"  46, \"Elevated Levels of Platelet Microparticles Are Associated with Disease Activity in Rheumatoid Arthritis.\" 1498 (2002)","DOI":"10.1002\/art.10312"},{"key":"3","doi-asserted-by":"crossref","unstructured":"A Ogawa  and H Matsubara  \"Thrombosis Research.\"  195, \"increased Levels of Platelet-Derived Microparticles in Pulmonary Hypertension.\" 120 (2020)","DOI":"10.1016\/j.thromres.2020.07.030"},{"key":"4","doi-asserted-by":"crossref","unstructured":"Siljander Prm,  \"Thrombosis Research.\"  127, \"Platelet-Derived Microparticles - An Updated Perspective.\" S30 (2011)","DOI":"10.1016\/S0049-3848(10)70152-3"},{"key":"5","doi-asserted-by":"crossref","unstructured":"Q Qi, B Yang, H Li, et al.  \"Pancreas.\"  49, \"Platelet Microparticles Regulate Neutrophil Extracellular Traps in Acute Pancreatitis.\" 1099 (2020)","DOI":"10.1097\/MPA.0000000000001631"},{"key":"6","doi-asserted-by":"crossref","unstructured":"H-C Lin, H-W Chang, S-H Hsiao, et al.  \"Transfusion and Apheresis Science: official Journal of The World Apheresis Association: official Journal of The European Society For Haemapheresis.\"  53, \"Platelet-Derived Microparticles Trigger Thp-1 Monocytic Cell Aggregation and Release of Pro-Coagulant Tissue Factor-Expressing Microparticles in Vitro.\" 246 (2015)","DOI":"10.1016\/j.transci.2015.10.002"},{"key":"7","doi-asserted-by":"crossref","unstructured":"S Dinkla, B Van Cranenbroek, Van Der Heijden Wa, et al.  \"Blood.\"  127, \"Platelet Microparticles inhibit Il-17 Production by Regulatory T Cells Through P-Selectin.\" 1976 (2016)","DOI":"10.1182\/blood-2015-04-640300"},{"key":"8","doi-asserted-by":"crossref","unstructured":"A Giacomazzi, M Degan, S Calabria, et al.  \"Frontiers in Pharmacology.\"  7, \"Antiplatelet Agents inhibit The Generation of Platelet-Derived Microparticles.\" 314 (2016)","DOI":"10.3389\/fphar.2016.00314"},{"key":"9","doi-asserted-by":"crossref","unstructured":"Z Li, Delaney Mk, Ka O'brien, et al.  \"Arteriosclerosis, Thrombosis, and Vascular Biology.\"  30, \"Signaling During Platelet Adhesion and Activation.\" 2341 (2010)","DOI":"10.1161\/ATVBAHA.110.207522"},{"key":"10","doi-asserted-by":"crossref","unstructured":"B Estevez  and X Du  \"Physiology.\"  32, \"New Concepts and Mechanisms of Platelet Activation Signaling.\" 162 (2017)","DOI":"10.1152\/physiol.00020.2016"},{"key":"11","doi-asserted-by":"crossref","unstructured":"R. Waksman,  \"Circulation.\"  100, \"Late Thrombosis after Radiation. Sitting On A Time Bomb.\" 780 (1999)","DOI":"10.1161\/01.CIR.100.8.780"},{"key":"12","doi-asserted-by":"crossref","unstructured":"C-H Du, Y-D Wu, K Yang, et al.  \"Military Medical Research.\"  10, \"Apoptosis-Resistant Megakaryocytes Produce Large and Hyperreactive Platelets in Response to Radiation injury.\" 66 (2023)","DOI":"10.1186\/s40779-023-00499-z"},{"key":"13","doi-asserted-by":"crossref","unstructured":"Wong Jyc, Filippi Ar, Dabaja Bs, et al.  \"International Journal of Radiation Oncology, Biology, Physics.\"  101, \"Total Body Irradiation: Guidelines From The international Lymphoma Radiation Oncology Group (Ilrog).\" 521 (2018)","DOI":"10.1016\/j.ijrobp.2018.04.071"},{"key":"14","doi-asserted-by":"crossref","unstructured":"S Misson-Yates, R Cunningham, R Gonzalez, et al.  \"The British Journal of Radiology.\"  96, \"Optimised Conformal Total Body Irradiation: A Heterogeneous Practice, So Where Next?\" 20220650 (2023)","DOI":"10.1259\/bjr.20220650"},{"key":"15","doi-asserted-by":"crossref","unstructured":"B Laffont, A Corduan, M Rousseau, et al.  \"Thrombosis and Haemostasis.\"  115, \"Platelet Microparticles Reprogram Macrophage Gene Expression and Function.\" 311 (2016)","DOI":"10.1160\/th15-05-0389"},{"key":"16","doi-asserted-by":"crossref","unstructured":"M Chimen, A Evryviadou, Box Cl, et al.  \"Haematologica.\"  105, \"Appropriation of Gpib\u03b1 From Platelet-Derived Extracellular Vesicles Supports Monocyte Recruitment in Systemic inflammation.\" 1248 (2020)","DOI":"10.3324\/haematol.2018.215145"},{"key":"17","doi-asserted-by":"crossref","unstructured":"T Kanaji, S Russell, J Ware,  \"Blood.\"  100, \"Amelioration of The Macrothrombocytopenia Associated with The Murine Bernard-Soulier Syndrome.\" 2102 (2002)","DOI":"10.1182\/blood-2002-03-0997"},{"key":"18","doi-asserted-by":"crossref","unstructured":"A Corken, Ghosh Sp, R Du, et al.  \"Radiotherapy and Oncology: Journal of The European Society For Therapeutic Radiology and Oncology.\"  167, \"Platelet Glycoprotein Ib\u03b1 Provides Radiation Protection.\" 143 (2022)","DOI":"10.1016\/j.radonc.2021.12.030"},{"key":"19","doi-asserted-by":"crossref","unstructured":"Welsh Ja, Pol E Van Der, Arkesteijn Gja, et al.  \"Journal of Extracellular Vesicles.\"  9, \"Miflowcyt-Ev: A Framework For Standardized Reporting of Extracellular Vesicle Flow Cytometry Experiments.\" 1713526 (2020)","DOI":"10.1080\/20013078.2020.1713526"},{"key":"20","doi-asserted-by":"crossref","unstructured":"M. Alarc\u00f3n,  \"Heliyon.\"  5, \"Generation of Platelet-Derived Microparticles Through The Activation of The Toll-Like Receptor 4.\" E01486 (2019)","DOI":"10.1016\/j.heliyon.2019.e01486"},{"key":"21","doi-asserted-by":"crossref","unstructured":"E Bir\u00f3, Akkerman Jwn, Hoek Fj, et al.  \"Journal of Thrombosis and Haemostasis.\"  3, \"The Phospholipid Composition and Cholesterol Content of Platelet-Derived Microparticles: A Comparison with Platelet Membrane Fractions.\" 2754 (2005)","DOI":"10.1111\/j.1538-7836.2005.01646.x"},{"key":"22","doi-asserted-by":"crossref","unstructured":"Dean Wl, Lee Mj, Cummins Td, et al.  \"Thrombosis and Haemostasis.\"  102, \"Proteomic and Functional Characterisation of Platelet Microparticle Size Classes.\" 711 (2009)","DOI":"10.1160\/TH09-04-243"},{"key":"23","doi-asserted-by":"crossref","unstructured":"Ponomareva Aa, Nevzorova Ta, Mordakhanova Er, et al.  \"Journal of Thrombosis and Haemostasis: Jth.\"  15, \"intracellular Origin and Ultrastructure of Platelet-Derived Microparticles.\" 1655 (2017)","DOI":"10.1111\/jth.13745"},{"key":"24","doi-asserted-by":"crossref","unstructured":"M Ranucci, T Aloisio, U Di Dedda, et al.  \"Plos One.\"  14, \"Gender-Based Differences in Platelet Function and Platelet Reactivity to P2y12 inhibitors.\" E0225771 (2019)","DOI":"10.1371\/journal.pone.0225771"},{"key":"25","doi-asserted-by":"crossref","unstructured":"M Phang, L Lincz, M Seldon, et al.  \"The Journal of Nutritional Biochemistry.\"  23, \"Acute Supplementation with Eicosapentaenoic Acid Reduces Platelet Microparticle Activity in Healthy Subjects.\" 1128 (2012)","DOI":"10.1016\/j.jnutbio.2011.06.006"},{"key":"26","doi-asserted-by":"crossref","unstructured":"F H\u00e9rodin, S Richard, N Grenier, et al.  \"Health Physics.\"  103, \"Assessment of Total- and Partial-Body Irradiation in A Baboon Model: Preliminary Results of A Kinetic Study including Clinical, Physical, and Biological Parameters.\" 143 (2012)","DOI":"10.1097\/HP.0b013e3182475e54"},{"key":"27","doi-asserted-by":"crossref","unstructured":"F H\u00e9rodin, L Roy, N Grenier, et al.  \"Experimental Hematology.\"  35, \"Antiapoptotic Cytokines in Combination with Pegfilgrastim Soon after Irradiation Mitigates Myelosuppression in Nonhuman Primates Exposed to High Irradiation Dose.\" 1172 (2007)","DOI":"10.1016\/j.exphem.2007.04.017"},{"key":"28","doi-asserted-by":"crossref","unstructured":"Shulman Ln, Tarbell Nj, E Storen, et al.  \"international Journal of Radiation Oncology, Biology, Physics.\"  42, \"Low-Dose Total Body Irradiation and G-Csf without Hematopoietic Stem Cell Support in The Treatment of Relapsed Or Refractory Acute Myelogenous Leukemia (Aml), Or Aml in Second Or Subsequent Remission.\" 1113 (1998)","DOI":"10.1016\/S0360-3016(98)00344-7"},{"key":"30","doi-asserted-by":"crossref","unstructured":"Moon Mj, A Rai, P Sharma, et al.  \"Proteomics.\"  24, \"Differential Effects of Physiological Agonists On The Proteome of Platelet-Derived Extracellular Vesicles.\" E2300391 (2024)","DOI":"10.1002\/pmic.202300391"},{"key":"31","doi-asserted-by":"crossref","unstructured":"A Sonoda, M Murata, D Ito, et al.  \"Stroke.\"  31, \"Association Between Platelet Glycoprotein Ibalpha Genotype and Ischemic Cerebrovascular Disease.\" 493 (2000)","DOI":"10.1161\/01.STR.31.2.493"},{"key":"32","doi-asserted-by":"crossref","unstructured":"G Al-Massarani, F Najjar, A Aljapawe, et al.  \"international Journal of Occupational Medicine and Environmental Health.\"  31, \"Evaluation of Circulating Microparticles in Healthy Medical Workers Occupationally Exposed to Ionizing Radiation: A Preliminary Study.\" 783 (2018)","DOI":"10.13075\/ijomeh.1896.01106"},{"key":"29","doi-asserted-by":"crossref","unstructured":"L Shao, W Feng, H Li, et al.  \"Blood.\"  123, \"Total body irradiation causes long-term mouse BM injury via induction of HSC premature senescence in an Ink4a- and Arf-independent manner.\" 3105 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