{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:51:24Z","timestamp":1791237084983,"version":"4.1.0"},"reference-count":27,"publisher":"Radiation Research Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Radiation Research"],"published-print":{"date-parts":[[2026,5,13]]},"abstract":"<jats:p>Traditionally, the dicentric chromosome assay is performed on large vessels with lymphocyte cultures of 3\u20135 mL. Our aim was to enhance usability in different settings, focusing on irradiating a thin cell layer to target cells with low-penetration radiation and to handle large-scale triage situations. To achieve this, protocols for irradiation, culture, and metaphase fixation using a MIDI format in 48-well plates were developed. Using 100 \u00b5L of isolated lymphocytes in these plates, we systematically optimized cell density, mitogenic stimulation, cell cycle arrest, and metaphase fixation. The semi-automated dicentric scoring with DCSore exhibited a linear-quadratic dose-response relationship up to 8 Gy. This dose-response curve was validated with irradiated and blinded blood samples. The MIDI format provides a reproducible, scaled-down alternative to the classic DCA protocol, enabling exposure of cells to low-penetration radiation and being suitable for both emergency triage and routine biodosimetry.<\/jats:p>","DOI":"10.1667\/rade-25-00244","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T10:49:21Z","timestamp":1785754161000},"page":"130-137","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of Dicentric Chromosome Analysis for Individual Radiation Biodosimetry in MIDI Format"],"prefix":"10.1667","volume":"206","author":[{"given":"Friedrike A.","family":"Lange","sequence":"first","affiliation":[{"name":"Bundeswehr Institute of Radiobiology affiliated to the University of Ulm, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Port","sequence":"additional","affiliation":[{"name":"Bundeswehr Medical Academy, Department F, Medical CBRN-Preparedness, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Beinke","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Radiobiology affiliated to the University of Ulm, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1243","reference":[{"key":"8","doi-asserted-by":"crossref","unstructured":"E Royba, I Shuryak, B Ponnaiya, et al.  \"Sci Rep.\"  14, \"Multiwell-based G0-PCC assay for radiation biodosimetry.\" 19789 (2024)","DOI":"10.1038\/s41598-024-69243-4"},{"key":"25","doi-asserted-by":"crossref","unstructured":"G Gruel, E Gr\u00e9goire, S Lecas, et al.  \"Radiat Res.\"  179, \"Biological dosimetry by automated dicentric scoring in a simulated emergency.\" 557 (2013)","DOI":"10.1667\/RR3196.1"},{"key":"18","doi-asserted-by":"crossref","unstructured":"A Hern\u00e1ndez, D Endesfelder, J Einbeck, et al.  \"Int J Radiat Biol.\"  99, \"Biodose Tools: an R shiny application for biological dosimetry.\" 1378 (2023)","DOI":"10.1080\/09553002.2023.2176564"},{"key":"20","unstructured":"\"2023: Radiological protection \u2014 Performance criteria for service laboratories performing biological dosimetry by cytogenetics \u2014 Dicentric assay.\"  2023"},{"key":"17","doi-asserted-by":"crossref","unstructured":"W. Merkle,  \"Radiat Environ Biophys.\"  21, \"Statistical methods in regression and calibration analysis of chromosome aberration data.\" 217 (1983)","DOI":"10.1007\/BF01323412"},{"key":"2","doi-asserted-by":"crossref","unstructured":"A Gkikoudi, G Manda, C Beinke, et al.  \"Biomolecules.\"  15, \"Synergistic effects of UVB and ionizing radiation on human non-malignant cells: Implications for ozone depletion and secondary cosmic radiation exposure.\" 83 (2025)","DOI":"10.3390\/biom15040536"},{"key":"3","doi-asserted-by":"crossref","unstructured":"L Mazzitelli-Fuentes, L Negrin, V Venier, et al.  \"Clin Transl Radiat Oncol.\"  1, \"Continuous \u03b2-particle exposure: A study of DNA damage in ex vivo peripheral blood mononuclear cells irradiation with radioiodine.\" 101040 (2025)","DOI":"10.1016\/j.ctro.2025.101040"},{"key":"6","doi-asserted-by":"crossref","unstructured":"AS Balajee, T Smith, T Ryan, et al.  \"Radiat Prot Dosim.\"  182, \"Development of a miniaturized version of dicentric chromosome assay tool for radiological triage.\" 139 (2018)","DOI":"10.1093\/rpd\/ncy127"},{"key":"10","doi-asserted-by":"crossref","unstructured":"E Beyls, A Baeyens, A Vral,  \"Int J Radiat Biol.\"  97, \"The cytokinesis-block micronucleus assay for cryopreserved whole blood.\" 1252 (2021)","DOI":"10.1080\/09553002.2021.1941378"},{"key":"11","doi-asserted-by":"crossref","unstructured":"AL Graf, C Beinke, M Port,  \"Int J Radiat Biol.\"  10, \"Dose-response of X-ray induced dicentrics determined by fully- & semi-automated DCScore evaluation after 3 h or 24 h colcemid treatment.\" 1 (2025)","DOI":"10.1080\/09553002.2025.2523283"},{"key":"13","unstructured":"JKR. Savage,  \"Curr Top Radiat Res.\"  6, \"Sites of radiation induced chromosome exchanges.\" 129 (1970)"},{"key":"14","doi-asserted-by":"crossref","unstructured":"DG. Papworth,  \"Computing.\"  31, \"Exact tests of fit for a poisson distribution.\" 33 (1983)","DOI":"10.1007\/BF02247935"},{"key":"16","doi-asserted-by":"crossref","unstructured":"D. Papworth,  \"Radiation Botany.\"  15, \"Curve fitting by maximum likelihood, Appendix to paper by J.R.K. Savage: Radiation-induced chromosomal aberrations in the plant Tradescantia. 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