{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:23:16Z","timestamp":1783592596624,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In order to identify neuroendocrine tumour-specific protein expression, we generated monoclonal antibodies (mAbs) with a tumour-related reaction pattern using a human insulinoma as immunogen. One of the generated mAbs (mAb 1D4) exhibited striking immunoreactivity against various neuroendocrine tumours without staining pancreatic islets of Langerhans. Furthermore, mAb 1D4 immunostained a characteristic subtype of hypothalamic neurones. Using two-dimensional (2-D) gel electrophoresis, mAb 1D4 immunoblotting and mass spectrometry, heat shock protein 70 (Hsp70) isoforms were identified as the mAb 1D4-specific antigen. In hypothalamic tissue, the presence of two different Hsp70 isoforms (Hsp70-8 and Hsp70-1) was revealed by 2-D gel immunoblots and consecutive mass spectrometric peptide analysis. In contrast, insulinoma and other neuroendocrine tumours displayed solely Hsp70-8 expression. Moreover, the tumour-specific presence of an additional mAb 1D4 immunoreactive protein of 40 kDa was observed in eight out of eight tested neuroendocrine tumours. For this variant, exclusively, peptides derived from the C terminus excluding the 299 amino-terminal residues were detected. In cultured tumour-derived fibroblasts, expression of the truncated Hsp70-8 subtype was not present. In conclusion, we have demonstrated a neuroendocrine tumour-specific expression pattern of Hsp70 isoforms and identified an as yet unknown N-terminally truncated Hsp70-8 variant.<\/jats:p>","DOI":"10.1677\/erc.0.0110377","type":"journal-article","created":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T18:44:39Z","timestamp":1102013079000},"page":"377-389","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-8","source":"Crossref","is-referenced-by-count":10,"title":["Heat shock protein 70 (Hsp70) subtype expression in neuroendocrine tissue and identification of a neuroendocrine tumour-specific Hsp70 truncation."],"prefix":"10.1530","volume":"11","author":[{"given":"B","family":"Zierhut","sequence":"first","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Mechtler","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Gartner","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Daneva","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Base","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Weissel","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Niederle","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Division of Clinical Endocrinology and Metabolism, University of Vienna, W\u221a\u00a7hringer G\u221a\u00bartel 18-20, A-1090 Vienna, Austria."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Endocrine-related cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/erc.bioscientifica.com\/view\/journals\/erc\/11\/2\/15163312.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/erc\/article-pdf\/11\/2\/377\/149254\/15163312.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/erc\/article-pdf\/11\/2\/377\/149254\/15163312.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T09:52:11Z","timestamp":1783590731000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/erc\/article\/11\/2\/377\/26576\/Heat-shock-protein-70-Hsp70-subtype-expression-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2004,6,1]]}},"URL":"https:\/\/doi.org\/10.1677\/erc.0.0110377","relation":{},"ISSN":["1479-6821","1351-0088"],"issn-type":[{"value":"1479-6821","type":"electronic"},{"value":"1351-0088","type":"print"}],"subject":[],"published":{"date-parts":[[2004,6,1]]}}}