{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:24:47Z","timestamp":1783513487600,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Membrane preparations (100 000 g pellet) of rabbit, baboon and tree shrew (Tupaia belangeri) uteri were studied for binding of [3H]prostaglandin E2 ([3H]PGE2). Unbound [3H]PGE2 was separated by filtration through Whatman GF\/F filters. Nonspecific binding was determined by the amount of radioactivity associated with the filters in the presence of a 100-fold excess of radioinert PGE2. PGE2 bound to membranes could be displaced by some other prostaglandin (PG) molecules: PGE1, 16,16-dimethyl-PGE2, PGA1 and PGF2\u03b1, but not by 6-keto-PGF1\u03b1, PGD2 or arachidonic acid. No PGE2 binding was detected using either membrane ghosts from red blood cells or liposomes. Apparent equilibrium of the binding was reached by 60 min. There was no difference in dissociation constant (Kd) values between rabbits of different reproductive stages (mean range\u00b1s.e.m. was from 4\u00b76\u00b10\u00b73 to 5\u00b75 \u00b1 1\u00b70 nm), but pregnant baboons showed a significantly lower value (3\u00b73\u00b10\u00b74 nm) than did cyclic animals (12\u00b70 \u00b1 2\u00b70 nm). Binding capacity (Bmax) values, in contrast, were different only between oestrous rabbits and other reproductive stages. The small amounts of [ill] tissue only permitted estimates of the Kd and Bmax values to be made; these were 3\u00b78 nm and 499 fmol\/mg protein for oestrous animals and 5\u00b74 nm and 674 fmol\/mg protein for animals on day 7 of pregnancy, assuming only one class of sites. The present results demonstrate the presence of specific binding sites for PGE2 in uteri from several species.<\/jats:p>","DOI":"10.1677\/jme.0.0030033","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T14:53:29Z","timestamp":1229352809000},"page":"33-42","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of prostaglandin E2 binding to uterine membranes from baboon, rabbit and tree shrew (\n                    <i>Tupaia belangeri<\/i>\n                    )"],"prefix":"10.1530","volume":"3","author":[{"given":"J. R.","family":"Hodam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Snabes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. J.","family":"Kuehl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J. K.","family":"Harper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/3\/1\/jme_3_1_005.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/3\/1\/33\/48677\/jme_3_1_005.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/3\/1\/33\/48677\/jme_3_1_005.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:07:58Z","timestamp":1783512478000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article\/3\/1\/33\/6679\/Characterization-of-prostaglandin-E2-binding-to"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,7,1]]}},"URL":"https:\/\/doi.org\/10.1677\/jme.0.0030033","relation":{},"ISSN":["1479-6813","0952-5041"],"issn-type":[{"value":"1479-6813","type":"electronic"},{"value":"0952-5041","type":"print"}],"subject":[],"published":{"date-parts":[[1989,7,1]]}}}