{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:16:31Z","timestamp":1783512991216,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We have elucidated the action of tri-iodothyronine (T3) on the synthesis and secretion of seven major plasma proteins in a human hepatoblastoma cell line, Hep G2, and established an in vitro experimental model of human liver cells for the study of the mechanism of the action of thyroid hormone.<\/jats:p>\n                  <jats:p>Hep G2 cells cultured in serum-free medium were treated with various concentrations of T3. During the first 24 h of T3 treatment, accumulation of \u03b1-fetoprotein in the medium was decreased in a dose-dependent manner (10\u221211-10\u22128m), and the inhibitory effect was enhanced during the second 24 h of T3 treatment. On the other hand, \u03b11-antitrypsin accumulation in the medium during the second 24 h of hormone treatment was decreased by T3 (10\u22129\u201310\u22128m), although no change in accumulation was observed during the first 24 h of T3 treatment.<\/jats:p>\n                  <jats:p>The newly synthesized [35S]Met-labelled \u03b11-acid glycoprotein was increased by T3 and reached 3\u00b74-fold within 37 h of 10\u22128m T3 treatment. The stimulatory effect increased time-dependently (4\u00b76fold after 61 h). In contrast, the synthesis of \u03b1-fetoprotein was reduced to half of that of the control after T3 treatment for 37 h.<\/jats:p>\n                  <jats:p>Although the content of newly synthesized [35S]\u03b11-antitrypsin was not affected by 10\u22128m T3 treatment during 3 days of hormone treatment, the accumulation of \u03b11-antitrypsin in the medium decreased to 87%; in contrast, total cellular newly synthesized \u03b11-antitrypsin increased to 105\u2013130% of that of the control. From these results, it is suggested that \u03b11-antitrypsin secretion might be suppressed by T3 treatment. However, T3 did not affect the accumulation of albumin, transferrin, fibronectin and \u03b12-macroglobulin in the medium throughout the experiments.<\/jats:p>\n                  <jats:p>It was shown that T3 has diverse control mechanisms on the synthesis and secretion of plasma proteins in Hep G2 cells: stimulation (\u03b11-acid glycoprotein) or inhibition (\u03b1-fetoprotein) of plasma protein synthesis, and inhibition of protein secretion (\u03b11-antitrypsin).<\/jats:p>","DOI":"10.1677\/jme.0.0140227","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T14:53:29Z","timestamp":1229352809000},"page":"227-235","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":3,"title":["Actions of 3,5,3\u2032-tri-iodothyronine on the synthesis and secretion of major plasma proteins by a human hepatoblastoma cell line (Hep G2)"],"prefix":"10.1530","volume":"14","author":[{"given":"M","family":"Kobayashi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Horiuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/14\/2\/jme_14_2_008.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/14\/2\/227\/46108\/jme_14_2_008.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/14\/2\/227\/46108\/jme_14_2_008.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T11:34:22Z","timestamp":1783510462000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article\/14\/2\/227\/5947\/Actions-of-3-5-3-tri-iodothyronine-on-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,4,1]]}},"URL":"https:\/\/doi.org\/10.1677\/jme.0.0140227","relation":{},"ISSN":["1479-6813","0952-5041"],"issn-type":[{"value":"1479-6813","type":"electronic"},{"value":"0952-5041","type":"print"}],"subject":[],"published":{"date-parts":[[1995,4,1]]}}}