{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:16:31Z","timestamp":1783512991221,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>It has been suggested that the thyroid itself may contribute to the inflammatory process observed in autoimmune thyroiditis by releasing the cytokines interleukin-1\u03b1 (IL-1\u03b1), interleukin-6 (IL-6) and interleukin-8 (IL-8), but studies of cytokine gene expression in thyrocytes have been limited and conflicting. A semi-quantitative reverse transcription-PCR technique has been used to investigate the expression of IL-1\u03b1, IL-6 and IL-8 mRNA in the human thyroid cell line HTori3 and in cultures of primary human thyroid follicular cells (TFCs). Cytokine mRNA levels were examined over a 24-h period, and the modulatory effects of exogenous IL-1\u03b1, interferon-\u03b3 (IFN-\u03b3) and TSH investigated. Basal expression of IL-1\u03b1, IL-6 and IL-8 mRNA was detected in HTori3 and primary TFC cultures. Stimulation with IL-1 (10 U\/ml) for 12 h produced an increase in the level of IL-1\u03b1 mRNA in both primary TFC and HTori3 cultures. IL-6 and IL-8 mRNA levels were increased by the addition of IL-1 in both cell types, and this effect was detected throughout the 24-h time-course. IFN-\u03b3 (100 U\/ml) had no significant effect on cytokine gene expression. A higher concentration of IFN-\u03b3 (500 U\/ml) had no significant effect on the expression of IL-1\u03b1 or IL-8 but produced an increase in the level of IL-6 mRNA in primary cultures and in HTori3 cells. Addition of TSH (1 mU\/ml) produced an increase in the level of IL-1\u03b1 mRNA in primary TFC and HTori3 cells, at 12 and 24 h. TSH had no significant effect on the expression of IL-6 or IL-8 mRNA. These results demonstrate that human TFCs constitutively express IL-1\u03b1, IL-6 and IL-8 mRNA and that this expression can be modulated by IL-1, IFN-\u03b3 and TSH.<\/jats:p>","DOI":"10.1677\/jme.0.0150011","type":"journal-article","created":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T14:53:29Z","timestamp":1229352809000},"page":"11-21","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":31,"title":["Semi-quantitative analysis of interleukin-1\u03b1, interleukin-6 and interleukin-8 mRNA expression by human thyrocytes"],"prefix":"10.1530","volume":"15","author":[{"given":"P F","family":"Watson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A P","family":"Pickerill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Davies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A P","family":"Weetman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/15\/1\/jme_15_1_002.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/15\/1\/11\/46117\/jme_15_1_002.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/15\/1\/11\/46117\/jme_15_1_002.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T11:34:16Z","timestamp":1783510456000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article\/15\/1\/11\/5946\/Semi-quantitative-analysis-of-interleukin-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,8,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,8,1]]}},"URL":"https:\/\/doi.org\/10.1677\/jme.0.0150011","relation":{},"ISSN":["1479-6813","0952-5041"],"issn-type":[{"value":"1479-6813","type":"electronic"},{"value":"0952-5041","type":"print"}],"subject":[],"published":{"date-parts":[[1995,8,1]]}}}