{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:17:19Z","timestamp":1783513039683,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>We have shown previously that expression of 46 and 54 kDa human myometrial G\u03b1s protein isoforms is increased during gestation and then subsequently decreased during labour. These proteins appear to be coded for by G\u03b1s-Small (with a serine residue at position 72) and G\u03b1s-Large (with a serine residue at position 87) mRNA splice variants respectively. In the study presented here we have used a G\u03b1s cDNA template to generate [32P]cytidine cRNA ribo-probes for use in RNase protection assays, so as to measure total myometrial G\u03b1s mRNA levels in relation to the pattern of expression of G\u03b1s mRNA splice variants during pregnancy and labour. We report that total levels of human myometrial G\u03b1s mRNA remain similar in non-pregnant and pregnant women but are substantially reduced during parturition. Our data also provide strong evidence that alternative splicing of G\u03b1s precursor mRNA has a primary role in regulating expression of G\u03b1s protein isoforms during pregnancy and labour. The inclusion of an additional serine codon in G\u03b1s mRNAs during pregnancy involves a switch in alternative splicing pathways. We speculate that this switch may be due to a change in specificity of splicing factors that are modulated during pregnancy and labour.<\/jats:p>","DOI":"10.1677\/jme.0.0180015","type":"journal-article","created":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T18:43:59Z","timestamp":1102013039000},"page":"15-25","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":9,"title":["Expression of human myometrial G\u03b1s messenger ribonucleic acid transcript during pregnancy and labour: involvement of alternative splicing pathways"],"prefix":"10.1530","volume":"18","author":[{"given":"G N","family":"Europe-Finner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Phaneuf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Cartwright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H J","family":"Mardon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A L\u00f3pez","family":"Bernal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Molecular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/jme.bioscientifica.com\/view\/journals\/jme\/18\/1\/jme_18_1_003.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/18\/1\/15\/46564\/jme_18_1_003.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article-pdf\/18\/1\/15\/46564\/jme_18_1_003.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T11:39:27Z","timestamp":1783510767000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/jme\/article\/18\/1\/15\/6060\/Expression-of-human-myometrial-G-s-messenger"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,2,1]]}},"URL":"https:\/\/doi.org\/10.1677\/jme.0.0180015","relation":{},"ISSN":["1479-6813","0952-5041"],"issn-type":[{"value":"1479-6813","type":"electronic"},{"value":"0952-5041","type":"print"}],"subject":[],"published":{"date-parts":[[1997,2,1]]}}}