{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:45:04Z","timestamp":1791297904528,"version":"4.1.0"},"reference-count":35,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The best characterized effect of glucose-dependent insulinotropic polypeptide (GIP) is its stimulatory effect on insulin secretion by pancreatic \u03b2-cells. Recently, it was demonstrated that some cases of primary adrenal Cushing\u2019s syndrome were secondary to the ectopic expression of non-mutated GIP receptor (GIP-R) in bilateral adrenal hyperplasias or unilateral adrenal adenomas, resulting in food-dependent steroidogenesis. Using a human multiple-expression tissue array, GIP-R was found to be expressed in a large number of human adult and fetal tissues, but not in the adrenal gland. The analysis of the promoter region of human (h) GIP-R gene revealed six consensus sequences important in regulating the reporter gene activity and capable of binding to Sp1 and Sp3 transcription factors. Data obtained by gene array and semi-quantitative RT-PCR showed an increase in the expression of Sp3 and CRSP9 (co-regulator of Sp1 transcription factor, subunit 9) in the adrenal adenomas or bilateral macronodular hyperplasias of patients with GIP-dependent Cushing\u2019s syndrome; they were, however, also increased in some patients with non-GIP-dependent cortisol-secreting adenomas or with ACTH-dependent Cushing\u2019s disease. This study represents the first step in our understanding of the mechanisms involved in the regulation of the expression of the hGIP-R gene.<\/jats:p>","DOI":"10.1677\/jme.1.01765","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T13:37:55Z","timestamp":1123508275000},"page":"61-71","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":32,"title":["The Sp transcription factors are involved in the cellular expression of the human glucose-dependent insulinotropic polypeptide receptor gene and overexpressed in adrenals of patients with Cushing\u2019s syndrome"],"prefix":"10.1530","volume":"35","author":[{"given":"Vale\u0301rie","family":"Baldacchino","sequence":"first","affiliation":[{"name":"Laboratory of Endocrine Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Oble","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrine Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick-Olivier","family":"De\u0301carie","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrine Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Bourdeau","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrine Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Hamet","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology of Hypertension and Molecular Medicine, Department of Medicine, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al, Montreal, Quebec, Canada 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanne","family":"Tremblay","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology of Hypertension and Molecular Medicine, Department of Medicine, Centre Hospitalier de l\u2019Universite\u0301 de Montre\u0301al, Montreal, Quebec, Canada 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301","family":"Lacroix","sequence":"additional","affiliation":[{"name":"Laboratory of Endocrine Pathophysiology and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2026070811423204400_ANDREWS-AND-FALLER-1991","doi-asserted-by":"crossref","unstructured":"Andrews\n              NC\n            \n           & Faller DV 1991 A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. 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