{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:11:57Z","timestamp":1791090717238,"version":"4.1.0"},"reference-count":31,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recent studies have revealed that hundreds of genes in the uterus are activated by estrogen. Their expression profiles differ over time and doses and it is not clear whether all these genes are directly regulated by estrogen via the estrogen receptor. To select the genes that may be regulated by estrogen, we treated mice with several doses of estrogen and searched for those genes whose dose\u2013response expression pattern mirrored the uterine growth pattern. Among those genes, we found that the dose-dependent expression of the adrenomedullin (ADM) gene correlated well with the uterotrophic effect of estrogen. ADM expression is induced early after estrogen administration and is restricted to the endometrial stroma. The spatiotemporal gene expression pattern of ADM was similar to that of receptor-modifying protein 3 (RAMP3). RAMP3 is known to modify calcitonin gene-related receptor (CRLR) so that it can then serve as an ADM receptor. Chromatin immunoprecipitation assays indicated that the estrogen receptor binds directly to the ADM promoter region and RAMP3 intron after estrogen administration. It was also shown that neither the ADM nor RAMP3 gene could be activated in estrogen receptor-\u03b1-null mouse. Although uterine ADM expression has been reported to occur in the myometrium, our observations indicate that estrogen-induced ADM is also expressed in the uterine stroma and that such variable, spatiotemporally regulated ADM expression contributes to a wider range of biological effects than previously expected.<\/jats:p>","DOI":"10.1677\/jme.1.01825","type":"journal-article","created":{"date-parts":[[2006,2,6]],"date-time":"2006-02-06T17:27:36Z","timestamp":1139246856000},"page":"81-89","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":52,"title":["The estrogen-responsive adrenomedullin and receptor-modifying protein 3 gene identified by DNA microarray analysis are directly regulated by estrogen receptor"],"prefix":"10.1530","volume":"36","author":[{"given":"H","family":"Watanabe","sequence":"first","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Science, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan and Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Science, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan and Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Science, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan and Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Goto","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Science, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan and Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Krust","sequence":"additional","affiliation":[{"name":"Institut de Ge\u0301ne\u0301tique et de Biologie Mole\u0301culaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Universite\u0301 Louis Pasteur, Colle\u0301ge de France, 67404 Illkirch, Strasbourg, France 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Chambon","sequence":"additional","affiliation":[{"name":"Institut de Ge\u0301ne\u0301tique et de Biologie Mole\u0301culaire et Cellulaire, Centre National de la Recherche Scientifique, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale, Universite\u0301 Louis Pasteur, Colle\u0301ge de France, 67404 Illkirch, Strasbourg, France 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Iguchi","sequence":"additional","affiliation":[{"name":"Okazaki Institute for Integrative Bioscience, National Institutes of Natural Science, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan and Core Research for Evolution Science and Technology (CREST), Japan Science and Technology Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2026070811383673100_CAMERON-ETAL-2002","doi-asserted-by":"crossref","unstructured":"Cameron\n            VA\n          , Autelitano DJ, Evans JJ, Ellmers LJ, Espiner EA, Nicholls MG & Richards AM 2002 Adrenomedullin expression in rat uterus is correlated with plasma estradiol. American Journal of Physiology Endocrinology & Metabolism282E139\u2013E146.","DOI":"10.1152\/ajpendo.2002.282.1.E139"},{"key":"2026070811383673100_CORNISH-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Cornish\n            J\n          , Callon KE, Coy DH, Jiang NY, Xiao L, Cooper GJ & Reid IR 1997 Adrenomedullin is a potent stimulator of osteoblastic activity in vitro and in vivo. American Journal of Physiology Endocrinology & Metabolism273E1113\u2013E1120.","DOI":"10.1152\/ajpendo.1997.273.6.E1113"},{"key":"2026070811383673100_CORNISH-ETAL-2003","unstructured":"Cornish\n            J\n          , Naot D & Reid IR 2003 Adrenomedullin\u2013a regulator of bone formation. Regulatory Peptides11279\u201386."},{"key":"2026070811383673100_DI-IORIO-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Di Iorio\n            R\n          , Marinoni E, Letizia C, Villaccio B, Alberini A & Cosmi EV 1999 Adrenomedullin production is increased in normal human pregnancy. 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European Journal of Pharmacology371159\u2013167.","DOI":"10.1016\/S0014-2999(99)00151-X"},{"key":"2026070811383673100_MARTINEZ-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Martinez\n            A\n          , Miller MJ, Catt KJ & Cuttitta F 1997 Adrenomedullin receptor expression in human lung and in pulmonary tumors. Journal of Histochemistry & Cytochemistry45159\u2013164.","DOI":"10.1177\/002215549704500202"},{"key":"2026070811383673100_MCLATCHIE-ETAL-1998","doi-asserted-by":"crossref","unstructured":"McLatchie\n            LM\n          , Fraser NJ, Main MJ, Wise A, Brown J, Thompson N, Solari R, Lee MG & Foord SM 1998 RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor. 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