{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:17:19Z","timestamp":1783556239927,"version":"3.55.0"},"reference-count":35,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The level of thyrotropin (TSH) secretion is determined by the balance of TSH-releasing hormone (TRH) and thyroid hormones. However, neuromedin B (NB), a bombesin-like peptide, highly concentrated in the pituitary, has been postulated to be a tonic inhibitor of TSH secretion. We studied the pituitary\u2013thyroid axis in adult male mice lacking NB receptor (NBR-KO) and their wild-type (WT) littermates. At basal state, NBR-KO mice presented serum TSH slightly higher than WT (18%, P&amp;lt; 0.05), normal intra-pituitary TSH content, and no significant changes in \u03b1 and \u03b2 TSH mRNA levels. Serum thyroxine was normal but serum triiodothyronine (T3) was reduced by 24% (P&amp;lt; 0.01) in NBR-KO mice. Pituitaries of NBR-KO mice exhibited no alteration in prolactin mRNA expression but type I and II deiodinase mRNA levels were reduced by 53 and 42% respectively (P&amp;lt; 0.05), while TRH receptor mRNA levels were importantly increased (78%, P&amp;lt; 0.05). The TSH-releasing effect of TRH was significantly higher in NBR-KO than in WT mice (7.1-and 4.0-fold respectively), but, while WT mice presented a 27% increase in serum T3 (P&amp;lt; 0.05) after TRH, NBR-KO mice showed no change in serum T3 after TRH. NBR-KO mice did not respond to exogenous NB, while WT showed a 30% reduction in serum TSH. No compensatory changes in mRNA expression of NB or other bombesin-related peptides and receptors (gastrin-releasing peptide (GRP), GRP-receptor and bombesin receptor subtype-3) were found in the pituitary of NBR-KO mice. Therefore, the data suggest that NB receptor pathways are importantly involved in thyrotroph gene regulation and function, leading to a state where TSH release is facilitated especially in response to TRH, but probably with a less-bioactive TSH. Therefore, the study highlights the important role of NB as a physiological regulator of pituitary\u2013thyroid axis function and gene expression.<\/jats:p>","DOI":"10.1677\/jme.1.01892","type":"journal-article","created":{"date-parts":[[2006,2,6]],"date-time":"2006-02-06T17:27:36Z","timestamp":1139246856000},"page":"73-80","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-6","source":"Crossref","is-referenced-by-count":34,"title":["Disruption of neuromedin B receptor gene results in dysregulation of the pituitary\u2013thyroid axis"],"prefix":"10.1530","volume":"36","author":[{"given":"K J","family":"Oliveira","sequence":"first","affiliation":[{"name":"Laborato\u0301rio de Endocrinologia Molecular, Instituto de Biofi\u0301sica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T M","family":"Ortiga-Carvalho","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Endocrinologia Molecular, Instituto de Biofi\u0301sica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Cabanelas","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Endocrinologia Molecular, Instituto de Biofi\u0301sica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A L C","family":"Veiga","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Endocrinologia Molecular, Instituto de Biofi\u0301sica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Ohki-Hamazaki","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Wada","sequence":"additional","affiliation":[{"name":"Department of Degenerative Neurological Diseases, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C C","family":"Pazos-Moura","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Endocrinologia Molecular, Instituto de Biofi\u0301sica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2026070811384223000_AOKI-ETAL-2002","doi-asserted-by":"crossref","unstructured":"Aoki\n            K\n          , Sun YJ, Aoki S, Wada K & Wada E 2002 Cloning, expression, and mapping of a gene that is up regulated in adipose tissue of mice deficient in bombesin receptor subtype-3. 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