{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:45:20Z","timestamp":1784641520448,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:p>\n                    Data on the involvement of aldosterone in the regulation of the renin\u2013angiotensin\u2013aldosterone system (RAAS) in rodents are still scarce, partly due to the high sample volumes needed by commercially available assays and to the very low aldosterone concentrations present. We have developed a highly sensitive and non-isotopic immunoassay, requiring a volume of only 50\u200a\u03bcl serum for a duplicate measurement, employing a highly specific monoclonal antibody against aldosterone. The assay was validated in human and mouse samples and exhibited a linear working range from 10 to 1000\u200apg\/ml. Values obtained after a chromatographic purification step correlated significantly to the dichloromethane extraction ordinarily used. Basal aldosterone values were measured in 75 mouse hybrids and found within the linear range (173\u00b121\u200apg\/ml), with no significant difference between males and females. Additionally, we show an increase in serum aldosterone in mice from 3 to 11 weeks of age. Mice of the same genetic background were treated with dexamethasone intraperitoneally (\n                    <jats:italic>n<\/jats:italic>\n                    =7), resulting in significantly decreased concentrations (35\u00b13 vs 114\u00b133\u200apg\/ml in controls;\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001). In contrast, adrenocorticotropic hormone resulted in significantly increased serum aldosterone (603\u00b1119\u200apg\/ml;\n                    <jats:italic>n<\/jats:italic>\n                    =7;\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.001), as did the physiological stimulation of the RAAS by a high K\n                    <jats:sup>+<\/jats:sup>\n                    \/low Na\n                    <jats:sup>+<\/jats:sup>\n                    diet (1369\u00b1703 vs 172\u00b136\u200apg\/ml). In conclusion, we have developed and validated an extremely sensitive assay for determination of aldosterone concentrations from very small serum samples, which could be especially useful in pharmacological intervention studies in rodent models.\n                  <\/jats:p>","DOI":"10.1677\/joe-07-0134","type":"journal-article","created":{"date-parts":[[2008,2,5]],"date-time":"2008-02-05T19:48:08Z","timestamp":1202240888000},"page":"215-224","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":35,"title":["A highly sensitive immunofluorometric assay for the measurement of aldosterone in small sample volumes: validation in mouse serum"],"prefix":"10.1530","volume":"196","author":[{"given":"J","family":"Manolopoulou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bielohuby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S J","family":"Caton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C E","family":"Gomez-Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I","family":"Renner-Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Wolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U D","family":"Lichtenauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Beuschlein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Hoeflich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bidlingmaier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/196\/2\/215.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/196\/2\/215.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:53:01Z","timestamp":1784555581000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/196\/2\/215-224\/21562"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11,2]]},"references-count":0,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.1677\/joe-07-0134","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,11,2]]}}}