{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:07Z","timestamp":1784557087207,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,3]]},"abstract":"\n Hyperstimulation of ovarian function with human FSH (hFSH) attenuates the preovulatory surge of LH. These experiments aimed at investigating the mechanism of ovarian-mediated FSH suppression of the progesterone (P\n 4<\/jats:sub>\n ) receptor (PR)-dependent LH surge in the rat. Four-day cycling rats were injected with hFSH, oestradiol benzoate (EB) or vehicle during the dioestrous phase. On pro-oestrus, their pituitaries were studied for PR mRNA and protein expression. Additionally, pro-oestrous pituitaries were incubated in the presence of oestradiol-17\u03b2 (E\n 2<\/jats:sub>\n ), and primed with P\n 4<\/jats:sub>\n and LH-releasing hormone (LHRH), with or without the antiprogestin RU486. After 1\u200ah of incubation, pituitaries were either challenged or not challenged with LHRH. Measured basal and LHRH-stimulated LH secretions and LHRH self-priming were compared with those exhibited by incubated pituitaries on day 4 from ovariectomized (OVX) rats in metoestrus (day 2) injected with hFSH and\/or EB on days 2 and 3. The results showed that: i) hFSH lowered the spontaneous LH surge without affecting basal LH and E\n 2<\/jats:sub>\n levels, gonadotroph PR-A\/PR-B mRNA ratio or immunohistochemical protein expression; ii) incubated pro-oestrous pituitaries from hFSH-treated rats did not respond to P\n 4<\/jats:sub>\n or LHRH, and lacked E\n 2<\/jats:sub>\n -augmenting and LHRH self-priming effects and iii) OVX reversed the inhibitory effects of hFSH on LH secretion. It is concluded that under the influence of hFSH, the ovaries produce a non-steroidal factor which suppresses all PR-dependent events of the LH surge elicited by E\n 2<\/jats:sub>\n . The action of such a factor seemed to be due to a blockade of gonadotroph PR action rather than to an inhibition of PR expression.\n <\/jats:p>","DOI":"10.1677\/joe-07-0223","type":"journal-article","created":{"date-parts":[[2008,2,29]],"date-time":"2008-02-29T13:52:07Z","timestamp":1204293127000},"page":"583-592","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":9,"title":["The ovary-mediated FSH attenuation of the LH surge in the rat involves a decreased gonadotroph progesterone receptor (PR) action but not PR expression"],"prefix":"10.1530","volume":"196","author":[{"given":"Ana","family":"Gordon","sequence":"first","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 C","family":"Garrido-Gracia","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmina","family":"Bellido","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A Garc\u00eda","family":"Velasco","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Millan","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Tena-Sempere","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"Mart\u00edn de las Mulas","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E","family":"S\u00e1nchez-Criado","sequence":"additional","affiliation":[{"name":"Departments of1Cell BiologyPhysiology and Immunology and2Comparative PathologyUniversity of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain3IVI MadridMadrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/196\/3\/583.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/196\/3\/583.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:53:06Z","timestamp":1784555586000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/196\/3\/583-592\/21576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12,4]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1677\/joe-07-0223","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,12,4]]}}}