{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:18:55Z","timestamp":1784557135679,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:p>\n                    Prolactin (PRL) acts through its receptor (PRLR) via both endocrine and local paracrine\/autocrine pathways to regulate biological processes including reproduction and lactation. We analyzed the tissue- and stage of gestation-specific regulation of PRL and PRLR expression in various tissues of pigs. Abundance of p\n                    <jats:italic>PRLR<\/jats:italic>\n                    -long form (LF) mRNA increased in the mammary gland and endometrium during gestation while in other tissues it remained constant. There was a parallel increase in the abundance of the pPRLR-LF protein in the mammary gland and endometrium during gestation. We determined the hormonal regulation of p\n                    <jats:italic>PRLR<\/jats:italic>\n                    -LF mRNA expression in various tissues from ovariectomized, hypoprolactinemic gilts given combinations of the replacement hormones estrogen (E\n                    <jats:sub>2<\/jats:sub>\n                    ), progestin (P), and\/or haloperidol-induced PRL. Abundance of p\n                    <jats:italic>PRLR<\/jats:italic>\n                    -LF mRNA in kidney and liver was unaffected by hormone treatments. Expression of uterine p\n                    <jats:italic>PRLR<\/jats:italic>\n                    -LF mRNA was induced by E\n                    <jats:sub>2<\/jats:sub>\n                    whereas the effect of E\n                    <jats:sub>2<\/jats:sub>\n                    was abolished by co-administering P. The expression of p\n                    <jats:italic>PRLR<\/jats:italic>\n                    -LF mRNA in the mammary gland stroma was induced by PRL, whereas E\n                    <jats:sub>2<\/jats:sub>\n                    induced its expression in the epithelium. In contrast to these changes in p\n                    <jats:italic>PRLR<\/jats:italic>\n                    expression, p\n                    <jats:italic>PRL<\/jats:italic>\n                    expression was relatively constant and low during gestation in all tissues except the pituitary. Taken together, these data reveal that specific combinations of E\n                    <jats:sub>2<\/jats:sub>\n                    , P, and PRL differentially regulate pPRLR-LF expression in the endometrium and mammary glands, and that the action of PRL on its target tissues is dependent upon pPRLR-LF abundance more so than the local PRL expression.\n                  <\/jats:p>","DOI":"10.1677\/joe-08-0486","type":"journal-article","created":{"date-parts":[[2009,4,28]],"date-time":"2009-04-28T20:58:27Z","timestamp":1240952307000},"page":"153-166","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":28,"title":["Tissue-specific regulation of porcine prolactin receptor expression by estrogen, progesterone, and prolactin"],"prefix":"10.1530","volume":"202","author":[{"given":"Josephine F","family":"Trott","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine C","family":"Horigan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia M","family":"Gloviczki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristen M","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley A","family":"Freking","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Farmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Spencer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph E","family":"Morabito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell C","family":"Hovey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/202\/1\/153.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/202\/1\/153.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:54:34Z","timestamp":1784555674000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/202\/1\/153-166\/21912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,28]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1677\/joe-08-0486","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,28]]}}}