{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:30:25Z","timestamp":1788327025398,"version":"build-2803163510"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:p>\n                    Vitamins A (VA) and D (VD) are metabolised by vertebrates to bioactive retinoic acid (RA) and calcitriol (CTR). RA and CTR involvement in bone metabolism requires fine-tuned regulation of their synthesis and breakdown. In mammals antagonism of VA and VD is observed, but the mechanism of interaction is unknown. We investigated VA\u2013VD interactions in Atlantic salmon (\n                    <jats:italic>Salmo salar<\/jats:italic>\n                    L.) following i.p. injection of RA and\/or CTR. VA metabolites, CTR, calcium (Ca), magnesium (Mg) and phosphorus (P) were determined in plasma. Expression of bone matrix Gla protein (\n                    <jats:italic>mgp<\/jats:italic>\n                    ), collagen 1 alpha2 chain (\n                    <jats:italic>col1a2<\/jats:italic>\n                    ) and alkaline phosphatase (\n                    <jats:italic>alp<\/jats:italic>\n                    ) mRNA was quantified to reflect osteogenesis. Branchial epithelial Ca channel (\n                    <jats:italic>ecac<\/jats:italic>\n                    listed as\n                    <jats:italic>trpv6<\/jats:italic>\n                    in ZFIN Database) mRNA levels and intestinal Ca and P influx were determined to study Ca\/P handling targets of RA and CTR. RA-injection (with or without CTR) decreased plasma CTR-levels three- to sixfold. CTR injection did not affect RA metabolites, but lowered CTR in plasma 3 and 5 days after injection. Lowered plasma CTR correlated with decreased\n                    <jats:italic>mgp<\/jats:italic>\n                    and\n                    <jats:italic>col1a2<\/jats:italic>\n                    expression in all groups and with decreased\n                    <jats:italic>alp<\/jats:italic>\n                    in CTR-injected fish. RA-treated salmon had enhanced\n                    <jats:italic>alp<\/jats:italic>\n                    expression, irrespective of reduced plasma CTR. Expression of\n                    <jats:italic>ecac<\/jats:italic>\n                    and unidirectional intestinal influx of Ca were stimulated following RA\u2013CTR treatment. Plasma Ca, Mg and P were not affected by any treatment. The results suggest cross-talk of RA with the VD endocrine system in Atlantic salmon. Enhanced Ca flux and osteogenesis (\n                    <jats:italic>alp<\/jats:italic>\n                    transcription) in RA-treated fish and inhibition of\n                    <jats:italic>mgp<\/jats:italic>\n                    expression revealed unprecedented disturbance of Ca physiology in hypervitaminosis A.\n                  <\/jats:p>","DOI":"10.1677\/joe-09-0199","type":"journal-article","created":{"date-parts":[[2009,6,16]],"date-time":"2009-06-16T20:45:38Z","timestamp":1245185138000},"page":"473-482","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":19,"title":["Retinoic acid cross-talk with calcitriol activity in Atlantic salmon (Salmo salar)"],"prefix":"10.1530","volume":"202","author":[{"given":"R","family":"\u00d8rnsrud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E J","family":"Lock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C N","family":"Glover","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Flik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/202\/3\/473.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/202\/3\/473.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:54:50Z","timestamp":1784555690000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/202\/3\/473-482\/21863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,16]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1677\/joe-09-0199","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,16]]}}}