{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:32Z","timestamp":1784557172953,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:p>\n                    Disruption of the\n                    <jats:italic>Esr1<\/jats:italic>\n                    gene encoding estrogen receptor \u03b1 (ER\u03b1) by insertion of a neomycin resistance gene (neo) into exon 2 (\u03b1ERKO mice) was shown previously to cause infertility in male mice. While full-length ER\u03b1 protein was not expressed in \u03b1ERKO mice, alternative splicing resulted in the low-level expression of a truncated form lacking the N-terminus A\/B domain and containing the DNA- and ligand-binding domains. Thus, it was unclear whether the reproductive phenotype in \u03b1ERKO males was only due to the lack of full-length ER\u03b1 or was affected by the presence of the variant ER\u03b1 isoform. The present study examined male mice with deletion of exon 3 of\n                    <jats:italic>Esr1<\/jats:italic>\n                    gene, lacking the DNA-binding domain, and null for ER\u03b1 (Ex3\u03b1ERKO). Dilation of some seminiferous tubules was apparent in male Ex3\u03b1ERKO mice as early as postnatal day 10 and was pronounced in all tubules from day 20 onward. At 6 weeks of age, sperm numbers and sperm motility were lower in Ex3\u03b1ERKO mice than in wild-type (WT) mice, and the rete testis and efferent ductules were dilated. Mating studies determined that adult Ex3\u03b1ERKO males were infertile and failed to produce copulatory plugs. Serum testosterone levels and\n                    <jats:italic>Hsd17b3<\/jats:italic>\n                    and\n                    <jats:italic>Cyp17a1<\/jats:italic>\n                    transcript levels were significantly higher, but serum estradiol, progesterone, LH, and FSH levels and\n                    <jats:italic>Cyp19a1<\/jats:italic>\n                    transcript levels were not significantly different from those in WT mice. These results confirm and extend those seen in other studies on male mice with deletion of exon 3 of\n                    <jats:italic>Esr1<\/jats:italic>\n                    gene. In addition, the reproductive phenotype of male Ex3\u03b1ERKO mice recapitulated the phenotype of \u03b1ERKO mice, strongly suggesting that the \u03b1ERKO male infertility was not due to the presence of the DNA-binding domain in the truncated form of ER\u03b1 and that full-length ER\u03b1 is essential for maintenance of male fertility.\n                  <\/jats:p>","DOI":"10.1677\/joe-10-0290","type":"journal-article","created":{"date-parts":[[2010,9,10]],"date-time":"2010-09-10T21:48:51Z","timestamp":1284155331000},"page":"281-288","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":29,"title":["Ex3\u03b1ERKO male infertility phenotype recapitulates the \u03b1ERKO male phenotype"],"prefix":"10.1530","volume":"207","author":[{"given":"Eugenia H","family":"Goulding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia C","family":"Hewitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth S","family":"Korach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward M","family":"Eddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/207\/3\/281.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/207\/3\/281.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:55:17Z","timestamp":1784555717000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/207\/3\/281-288\/22015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,10]]},"references-count":0,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.1677\/joe-10-0290","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,10]]}}}