{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T12:32:03Z","timestamp":1783600323195,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>MRC Radioimmunoassay Team, 2 Forrest Road, Edinburgh, EH1 2QW<\/jats:p>\n                  <jats:p>(Received 9 May 1974)<\/jats:p>\n                  <jats:p>Luteinizing hormone releasing hormone (LH-RH) stimulates the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary. Radioimmunoassays have been developed in various laboratories to measure this decapeptide. In studies on man, Jeffcoate, Greenwood &amp; Holland (1974) found that 0\u00b775\u20132\u00b78% of an injected dose of LH-RH was excreted in the urine and that the excretion rate of endogenous LH-RH was about 1\u00b74 \u03bcg\/day, although the apparent daily excretion rate was reduced 50-fold using a more specific antiserum (Jeffcoate et al. 1974, note added in proof).<\/jats:p>\n                  <jats:p>A radioimmunoassay for LH-RH has been established using antiserum raised against a bovine serum albumin\u2013LH-RH conjugate (Jeffcoate, Fraser, Gunn &amp; Holland, 1973), given by Mr H. M. Fraser. After iodination of synthetic LH-RH (Hoechst), labelled peptide was separated from free iodide by gel filtration on Sephadex G-10. Material<\/jats:p>","DOI":"10.1677\/joe.0.0630255","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:08:26Z","timestamp":1229015306000},"page":"255-256","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":7,"title":["RADIOIMMUNOASSAY OF LUTEINIZING HORMONE RELEASING HORMONE-LIKE MATERIAL IN HUMAN URINE"],"prefix":"10.1530","volume":"63","author":[{"given":"A. E.","family":"BOLTON","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/63\/1\/joe_63_1_029.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/63\/1\/255\/131205\/joe_63_1_029.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/63\/1\/255\/131205\/joe_63_1_029.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:58:01Z","timestamp":1783598281000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/63\/1\/255\/23060\/RADIOIMMUNOASSAY-OF-LUTEINIZING-HORMONE-RELEASING"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1974,10,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.0630255","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1974,10,1]]}}}