{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:19:14Z","timestamp":1783610354989,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The incorporation of [3H]thymidine by rabbit chondrocytes in vitro has been developed as a sensitive assay for plasma somatomedin. A concentration of normal plasma of 2\u00b75% enhanced [3H]thymidine incorporation by 5- to 20-fold compared with basal levels in the absence of plasma. The mean potency of plasma from normal adult men was 0\u00b796 \u00b1 0\u00b71 u.\/ml (mean \u00b1 s.d.) and from acromegalic patients 1\u00b79 \u00b1 0\u00b74 u.\/ml. The apparent potency of hypopituitary plasma alone increased on heating which suggested the presence of heatlabile inhibitors of somatomedin activity. The potency of heated hypopituitary plasma (0\u00b76 \u00b1 0\u00b709 u.\/ml) remained significantly lower (P &amp;lt; 0\u00b701) than normal plasma. Human growth hormone (0\u00b71\u201320 \u03bcu.\/ml), bovine growth hormone (0\u00b75\u201320 \u03bcu.\/ml), insulin (0\u00b75\u20135 \u03bcu.\/ml) and glucose (0\u00b73\u20132 mmol\/l) had no direct effect on the incorporation of [3H]thymidine. Chondrocytes which had been previously stored frozen also showed a response to plasma somatomedin.<\/jats:p>","DOI":"10.1677\/joe.0.0740205","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:22:29Z","timestamp":1229016149000},"page":"205-212","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":14,"title":["AN ASSAY FOR PLASMA SOMATOMEDIN: [3H]THYMIDINE INCORPORATION BY ISOLATED RABBIT CHONDROCYTES"],"prefix":"10.1530","volume":"74","author":[{"given":"I. K.","family":"ASHTON","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J. O.","family":"FRANCIS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/74\/2\/joe_74_2_005.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/74\/2\/205\/132907\/joe_74_2_005.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/74\/2\/205\/132907\/joe_74_2_005.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:06:51Z","timestamp":1783609611000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/74\/2\/205\/23559\/AN-ASSAY-FOR-PLASMA-SOMATOMEDIN-3H-THYMIDINE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1977,8,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.0740205","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1977,8,1]]}}}