{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T16:13:54Z","timestamp":1783613634586,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>An inhibitor of 5\u03b1-reductase, the 17\u03b2-carboxylic acid derivative of testosterone (testosterone-17\u03b2CA), has been used to evaluate the importance of the 5\u03b1-reduction of testosterone in its action on the suppression of LH secretion in male rats. The potential of testosterone-17\u03b2CA to inhibit the formation of 5\u03b1-dihydrotestosterone (DHT) was first demonstrated in vitro. When homogenates of hypothalami or anterior pituitary glands were incubated with [3H]testosterone in the presence of a 50-fold excess of testosterone-17\u03b2CA, the formation of labelled DHT was inhibited by more than 80%. Adult male rats that had been castrated for 1\u20132 months were fitted with chronic intravenous catheters and implanted with silicone elastomer sheets: one group received one sheet, 0\u00b75\u20132\u00b70 cm2 in size containing 1\u00b76% testosterone, a second group received one 50 cm2 sheet containing 1\u00b76% testosterone-17\u03b2CA and a third group received two sheets, one sheet 50 cm2 in size containing 1\u00b76% testosterone-17\u03b2CA and the second ranging in size from 0\u00b75 to 2\u00b70 cm2 and containing 1\u00b76% testosterone. Blood was withdrawn daily from each rat over a 4\u20135 day period after implantation of the steroids and the level of LH in the plasma was measured by radioimmunoassay. The seminal vesicles and the ventral prostate gland were removed at autopsy on day 4 or 5; the weights of these organs were shown to have increased progressively as the size of the implant of testosterone increased. In contrast, the level of LH in the plasma was suppressed to a comparable extent by implants of testosterone between 0\u00b76 and 2 cm2, whereas a 0\u00b75 cm2 implant of testosterone had no effect. Implants of testosterone-17\u03b2CA alone did not influence the weight of the accessory organs or the level of LH. When testosterone-17\u03b2CA and testosterone were implanted together, the growth-promoting effect of the latter on the accessory sex organs was significantly reduced. The effectiveness of testosterone in suppressing the level of LH in the plasma of these animals was not influenced by the presence of testosterone-17\u03b2CA and in certain instances the level was raised.<\/jats:p>","DOI":"10.1677\/joe.0.0810209","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:22:29Z","timestamp":1229016149000},"page":"209-220","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":18,"title":["INHIBITION OF STEROID 5\u03b1-REDUCTASE\n                    <i>IN VIVO<\/i>\n                    : EFFECT ON SUPPRESSION OF LUTEINIZING HORMONE AND STIMULATION OF ACCESSORY SEX ORGANS BY TESTOSTERONE IN THE ORCHIDECTOMIZED RAT"],"prefix":"10.1530","volume":"81","author":[{"given":"LIDIA WEI LIU","family":"KAO","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JUDITH","family":"WEISZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/81\/2\/joe_81_2_005.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/81\/2\/209\/134106\/joe_81_2_005.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/81\/2\/209\/134106\/joe_81_2_005.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:25:19Z","timestamp":1783610719000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/81\/2\/209\/23963\/INHIBITION-OF-STEROID-5-REDUCTASE-IN-VIVO-EFFECT"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,5,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1979,5,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.0810209","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1979,5,1]]}}}