{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T16:17:14Z","timestamp":1783613834133,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1983,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Thyrotrophin releasing hormone (TRH)-immunoreactive peptides have been quantified in canine serum, hypothalamus, liver, pancreas, adrenal, thyroid, prostate, testis, epididymis and semen by TRH radioimmunoassay, SP-Sephadex C-25 cation exchange chromatography, Sephadex G-10 exclusion chromatography and high pressure liquid chromatography. The total concentration of TRH and TRH-like peptides was highest in the hypothalamus, followed by liver, adrenal, pancreas, thyroid, prostate, epididymis, testis and serum. All of the TRH immunoreactivity (TRH-IR) within extracts of the hypothalamus was due to TRH. On the other hand, nearly all of the TRH-IR of extracts of liver, thyroid, prostate, epididymis, testis and semen was due to TRH-homologous peptides. Adrenal and pancreatic extracts contained a greater proportion of TRH in relation to the TRH-homologous peptides. Extracts of dog serum and semen were found to contain a TRH-binding substance which reduced the retention of added TRH by cation exchangers. The half-time of disappearance (t\u00bd) of synthetic TRH incubated at 23 \u00b0C in 10% (w\/v) homogenates in 0\u00b715 m-NaCl\u20130\u00b705 m-phosphate buffer, pH 7\u00b75, ranged from 22\u00b110 (s.d.) min for liver to 120 \u00b158 min for thyroid. The short t\u00bd for TRH added to dog liver homogenates contrasted with a previous report that dog liver is essentially free of TRH-degrading activity.<\/jats:p>","DOI":"10.1677\/joe.0.0980299","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:45:06Z","timestamp":1229017506000},"page":"299-306","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":15,"title":["Thyrotrophin releasing hormone and related peptides in canine tissues"],"prefix":"10.1530","volume":"98","author":[{"given":"A. E.","family":"Pekary","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melvyn","family":"Richkind","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Hershman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/98\/3\/joe_98_3_001.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/98\/3\/299\/137100\/joe_98_3_001.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/98\/3\/299\/137100\/joe_98_3_001.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T16:10:08Z","timestamp":1783613408000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/98\/3\/299\/24939\/Thyrotrophin-releasing-hormone-and-related"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1983,9,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.0980299","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1983,9,1]]}}}