{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:15:43Z","timestamp":1783595743822,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Mice were immunized with a mixture of four steroid antigens: dehydroepiandrosterone (DHA), oestradiol, progesterone and testosterone linked to bovine serum albumin through the 7, 6, 11\u03b1 and 17\u03b2 positions respectively. The response to immunization varied widely with no one mouse producing an optimal response to all four steroids. In the two fusion experiments performed, antibodies to all four antigens were developed. Data are presented which show that the immune response of the spleen donor is related to the relative numbers and quality of the antibodies produced to each steroid. Despite the structural identity of the progesterone and testosterone haptens, antibodies elicited in response to their respective antigens could readily be distinguished from each other.<\/jats:p>\n                  <jats:p>From the large number of monoclonal antibodies obtained those most useful for radioimmunoassay were three high affinity antibodies to oestradiol and two antibodies raised against DHA but with high affinity for DHA sulphate.<\/jats:p>\n                  <jats:p>J. Endocr. (1984) 100, 367\u2013376<\/jats:p>","DOI":"10.1677\/joe.0.1000367","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:45:06Z","timestamp":1229017506000},"page":"367-376","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":25,"title":["Simultaneous production of monoclonal antibodies to dehydroepiandrosterone, oestradiol, progesterone and testosterone"],"prefix":"10.1530","volume":"100","author":[{"given":"V. E.","family":"Fantl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Y.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/100\/3\/joe_100_3_016.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/100\/3\/367\/94394\/joe_100_3_016.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/100\/3\/367\/94394\/joe_100_3_016.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:15:27Z","timestamp":1783592127000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/100\/3\/367\/15044\/Simultaneous-production-of-monoclonal-antibodies"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1984,3,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1000367","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1984,3,1]]}}}