{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:16:00Z","timestamp":1783595760756,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1984,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Granulosa cells of antral ovarian follicles from adult 5-day cyclic rats were cultured on each day of the cycle. The rat granulosa cell conditioned medium (rGCCM) was harvested and renewed on each day of a 4-day culture period. Inhibin-like activity and progesterone were estimated in rGCCM using an in-vitro bioassay system with dispersed rat anterior pituitary cells and radioimmunoassay respectively. Removal of steroids from rGCCM with dextran-coated charcoal was effective and did not significantly change the inhibin-like activity of the treated samples.<\/jats:p>\n                  <jats:p>On day 1 of culture the inhibin-like activity of rGCCM for each day of the oestrous cycle was 20\u201390% higher than on days 2, 3 and 4 of culture when low and constant levels were observed. Media collected after culture on days 1 and 2 from pro-oestrous cells contained larger amounts of inhibin-like activity than media collected on the other days of the cycle.<\/jats:p>\n                  <jats:p>On day 1 of culture, rGCCM from pro-oestrous cells contained higher concentrations of progesterone than that from cells collected on the other days of the cycle. On days 2, 3 and 4 progesterone levels in rGCCM were undetectable (&amp;lt; 320 pmol\/l) except in media from pro-oestrous cultures on day 2. Addition of FSH (62 \u03bcg\/l) to granulosa cell cultures in medium with or without 10% fetal calf serum (FCS) did not alter the inhibin-like activity of rGCCM from pro-oestrous cells. The presence of FCS maintained the production of inhibin-like activity since rGCCM from cells cultured without FCS was devoid of FSH-suppressing activity after 3 days of culture.<\/jats:p>\n                  <jats:p>It is concluded that material with inhibin-like activity is secreted at a higher rate by granulosa cells collected on the day of pro-oestrus than on any other day of the cycle. However, on the day of pro-oestrus, transport of inhibin-like activity from the follicle to the circulation is apparently impeded since this increased inhibin production is not reflected in a reduction in plasma FSH concentration.<\/jats:p>\n                  <jats:p>J. Endocr. (1984) 103, 77\u201384<\/jats:p>","DOI":"10.1677\/joe.0.1030077","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:45:06Z","timestamp":1229017506000},"page":"77-84","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":9,"title":["Inhibin-like activity in media from cultured rat granulosa cells collected throughout the oestrous cycle"],"prefix":"10.1530","volume":"103","author":[{"given":"H. J.","family":"Sander","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. C. M.","family":"van Leeuwen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. H.","family":"de Jong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/103\/1\/joe_103_1_011.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/103\/1\/77\/94573\/joe_103_1_011.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/103\/1\/77\/94573\/joe_103_1_011.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:19:24Z","timestamp":1783592364000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/103\/1\/77\/15129\/Inhibin-like-activity-in-media-from-cultured-rat"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,10,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1030077","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1984,10,1]]}}}