{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:22:50Z","timestamp":1783596170139,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1985,12,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A robust, rapid, sensitive and specific enzyme-linked immunosorbent assay (ELISA), using an in-house immunoglobulin-A-sub-class mouse monoclonal human-thyroglobulin antibody (WNSM2), with a sensitivity of 1\u00b751 pmol\/l has been established for the measurement of thyroglobulin in serum. Standard curves in varying dilutions of human serum were similar to standard curves obtained in serum-free medium, thus demonstrating no significant cross-reactivity with any serum proteins other than thyroglobulin. Levels of serum thyroglobulin detected by ELISA correlated significantly (r = 0\u00b793, P &amp;lt; 0\u00b7001) with those from a standardized and well-characterized radioimmunoassay. The coefficients of variation within and between ELISA assays were 3\u00b79 and 7\u00b71% respectively. Thyroglobulin was detectable in 87% of 54 normal subjects who had no history of thyroid or autoimmune disease, the mean (\u00b1s.d.) for this group being 15 \u00b1 6\u00b76 pmol\/l with a range of 1\u00b751\u201353 pmol\/l. Using this assay, levels of thyroglobulin were shown to be significantly (P &amp;lt; 0\u00b7002) increased in patients with untreated hyperthyroid Graves' disease compared with normal subjects.<\/jats:p>\n                  <jats:p>J. Endocr. (1985) 107, 383\u2013387<\/jats:p>","DOI":"10.1677\/joe.0.1070383","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:45:06Z","timestamp":1229017506000},"page":"383-387","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":3,"title":["An enzyme-linked immunosorbent assay for the measurement of thyroglobulin in human serum using mouse monoclonal antibodies"],"prefix":"10.1530","volume":"107","author":[{"given":"P.","family":"Kilduff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. G.","family":"Black","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"McGregor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/107\/3\/joe_107_3_010.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/107\/3\/383\/95430\/joe_107_3_010.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/107\/3\/383\/95430\/joe_107_3_010.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T10:33:58Z","timestamp":1783593238000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/107\/3\/383\/15446\/An-enzyme-linked-immunosorbent-assay-for-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1985,12,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1070383","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1985,12,1]]}}}