{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:59:19Z","timestamp":1791349159874,"version":"4.3.1"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Pheromaxein, the 16-androstene steroid-binding protein with a relative molecular mass of 15 000 was isolated in sub-milligram quantities from the submaxillary gland and saliva of the Gottingen miniature boar, after a fourfold purification involving the following methods: ultrafiltration for submaxillary gland cytosols and ethanol precipitation for saliva, Concanavalin-A-Sepharose affinity chromatography, sodium dodecyl sulphate polyacrylamide gel electrophoresis, 'Extractigel-D' affinity chromatography (to remove sodium dodecyl sulphate) and fast protein-liquid chromatography. Yields of purified pheromaxein obtained after fast protein-liquid chromatography represented 10\u201320% of total protein present in an ultrafiltrate of a submaxillary gland cytosol. Fast protein-liquid chromatography separated the \u03b1- and \u03b2-charge isomers of pheromaxein which were shown to have isoelectric points of 4\u00b778 and 5\u00b735 respectively on flat-bed isoelectric focusing. Some data are provided for the variable occurrence of the isomeric forms of pheromaxein in relation to different breeds of pig. Five 16-unsaturated steroids showed the highest binding to pheromaxein. Other steroids of the 5\u03b1- and 5\u03b2-androstane series also showed some binding to pheromaxein, i.e. 17\u03b2-hydroxy-5\u03b1-androstan-3-one (19\u00b72%), with 5\u03b1-androstan-3-one, which has a similar urinous odour to 5\u03b1-androst-16-en-3-one, showing the greatest binding (42\u00b76%) relative to 5\u03b1-androst-16-en-3-one (100%).<\/jats:p>\n                  <jats:p>J. Endocr. (1988) 118, 47\u201357<\/jats:p>","DOI":"10.1677\/joe.0.1180047","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:52:58Z","timestamp":1229017978000},"page":"47-NP","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":30,"title":["The isolation, purification and some properties of pheromaxein, the pheromonal steroid-binding protein, in porcine submaxillary glands and saliva"],"prefix":"10.1530","volume":"118","author":[{"given":"W. D.","family":"Booth","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/118\/1\/joe_118_1_008.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/118\/1\/47\/97976\/joe_118_1_008.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/118\/1\/47\/97976\/joe_118_1_008.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T11:10:27Z","timestamp":1783595427000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/118\/1\/47\/16292\/The-isolation-purification-and-some-properties-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,7,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1180047","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1988,7,1]]}}}