{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:22:42Z","timestamp":1783606962149,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Collagenase-isolated mouse islets were incubated with gastrin-releasing peptide (GRP). At 5\u00b76 mmol glucose\/1, 10 nmol GRP\/l increased the release of insulin (by 50%) and glucagon (by twofold), decreased the release of pancreatic polypeptide (by 35%), but did not significantly affect the release of somatostatin. At 16\u00b77 mmol glucose\/l, 10 nmol GRP\/l increased glucagon release (by fivefold) and decreased pancreatic polypeptide release (by 46%), without significantly altering insulin and somatostatin release. GRP (200 nmol\/l) did not affect insulin release by perifused mouse islets at 2\u00b78 mmol glucose\/l, but increased both first and second phase insulin release after a square wave increase in the glucose concentration to 11\u00b71 mmol\/l. At 5\u00b76 mmol glucose\/l, GRP (100 pmol\/1\u2013100 nmol\/l) increased (by 50\u201370%) insulin release by the RINm5F clonal cell line. GRP did not affect glucose oxidation or the cyclic adenosine monophosphate content of RINm5F cells. However, the intracellular free Ca2+ concentration of RINm5F cells was rapidly and transiently increased by GRP (maximum increase of 64% about 10 s after exposure to 1 \u03bcmol GRP\/l). The rise of intracellular free Ca2+ was approximately halved in the absence of extracellular Ca2+. The results suggest that GRP may contribute to the normal regulation of the endocrine pancreas. The insulin-releasing effect of GRP is mediated via increased cytosolic free Ca2+, derived both from an increased net influx of extracellular Ca2+ and from mobilization of intracellular Ca2+ stores.<\/jats:p>\n                  <jats:p>Journal of Endocrinology (1990) 127, 335\u2013340<\/jats:p>","DOI":"10.1677\/joe.0.1270335","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T18:11:20Z","timestamp":1229019080000},"page":"335-340","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":7,"title":["Effect of gastrin-releasing peptide on the secretion of mouse islet hormones\n                    <i>in vitro<\/i>"],"prefix":"10.1530","volume":"127","author":[{"given":"L. C.","family":"Wilkes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Bailey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. G.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Conlon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. D.","family":"Buchanan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/127\/2\/joe_127_2_020.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/127\/2\/335\/99872\/joe_127_2_020.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/127\/2\/335\/99872\/joe_127_2_020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:06:37Z","timestamp":1783605997000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/127\/2\/335\/16938\/Effect-of-gastrin-releasing-peptide-on-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,11,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1270335","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1990,11,1]]}}}