{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T15:12:52Z","timestamp":1783609972346,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"3","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The effect of epidermal growth factor (EGF) on testicular germ cell differentiation was investigated. Testicular fragments from surgically prepared cryptorchid testes of adult mice were cultured for 9 days in serum-free media containing various concentrations of EGF. Histological sections of testis were examined under a light microscope and each type of germ cell and mitotic cell in the seminiferous tubules was counted per 1000 Sertoli cells. EGF at concentrations ranging from 100 to 200 ng\/ml induced differentiation of type A spermatogonia. The observed maximal stimulatory activity of EGF at a concentration of 100 ng\/ml was 30% of the positive control cultures treated with calf serum. EGF at concentrations ranging from 1 to 100 ng\/ml significantly inhibited the mitotic activity of FSH, FSH plus retinol, or FSH plus fetuin on type A spermatogonia and their differentiation. The number of type A spermatogonia in testes cultured with FSH, FSH plus retinol, or FSH plus fetuin decreased when EGF was added. On the other hand, EGF stimulated the differentiation of type A spermatogonia induced with fetuin but did not influence retinol-induced differentiation. It is proposed that EGF inhibits testicular germ cell differentiation by blocking the proliferation of type A spermatogonia stimulated by FSH.<\/jats:p>\n                  <jats:p>Journal of Endocrinology (1991) 128, 383\u2013388<\/jats:p>","DOI":"10.1677\/joe.0.1280383","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T18:11:20Z","timestamp":1229019080000},"page":"383-388","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":28,"title":["Differential effects of epidermal growth factor on the differentiation of type A spermatogonia in adult mouse cryptorchid testes\n                    <i>in vitro<\/i>"],"prefix":"10.1530","volume":"128","author":[{"given":"T.","family":"Haneji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. S.","family":"Koide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Tajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Nishimune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/128\/3\/joe_128_3_008.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/128\/3\/383\/100404\/joe_128_3_008.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/128\/3\/383\/100404\/joe_128_3_008.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:14:11Z","timestamp":1783606451000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/128\/3\/383\/17104\/Differential-effects-of-epidermal-growth-factor-on"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,3,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1280383","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1991,3,1]]}}}