{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:03:26Z","timestamp":1791086606516,"version":"4.1.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The effects of tri-iodothyronine (T3) and insulin-like growth factor-I (IGF-I) on [3H]thymidine incorporation, alkaline phosphatase (ALP) activity and IGF-I receptor mRNA levels were studied in rat epiphyseal chondrocytes cultured in monolayer.<\/jats:p>\n                  <jats:p>Chondrocytes from enzymatically digested rat tibia epiphyseal growth plates were seeded in monolayer culture and precultured for 7\u201314 days in Ham's F-12 medium supplemented with 10% (v\/v) newborn calf serum and 1% (v\/v) of a serum substitute. After preculture the medium was changed to Ham's F-12 medium containing 1% (v\/v) serum from hypophysectomized rats, and the effects of T3 and\/or IGF-I on DNA synthesis ([3H]thymidine incorporation), ALP activity (a late marker of differentiated epiphyseal chondrocytes) and IGF-I receptor mRNA levels were studied.<\/jats:p>\n                  <jats:p>ALP activity was increased by T3 in a dose-dependent manner with a maximal response at 10 \u03bcg T3\/1 (678 \u00b186% compared with control culture). The increase in ALP activity was accompanied by a concomitant decrease in [3H]thymidine incorporation (52 \u00b114% compared with control culture). Human GH (hGH; 50 \u03bcg\/l) and IGF-I (25 \u03bcg\/l) had no stimulatory effect on ALP activity. However IGF-I (10 \u03bcg\/l) exerted an inhibition on the T3 (10 \u03bcg\/l)-induced increase in ALP activity (64 \u00b1 9% compared with T3-treated culture). T3 (3 \u03bcg\/l) inhibited the increase in [3H]thymidine incorporation caused by 25 \u03bcg IGF-I\/l(51 \u00b1 13% compared with IGF-I-treated culture). Furthermore, IGF-I receptor mRNA levels were increased by 10 \u03bcg T3\/l (137 \u00b14\u00b72% compared with control culture) while no effect of hGH (50 \u03bcg\/l) or IGF-I (25 \u03bcg\/l) was demonstrated.<\/jats:p>\n                  <jats:p>Both T3 and IGF-I were shown to interact with epiphyseal chondrocytes and both substances seemed to affect cell proliferation and maturation and therefore longitudinal bone growth. Furthermore, the results indicated that IGF-I is important for proliferation of the cells while T3 initiates the terminal differentiation of epiphyseal chondrocytes.<\/jats:p>\n                  <jats:p>Journal of Endocrinology (1992) 135, 115\u2013123<\/jats:p>","DOI":"10.1677\/joe.0.1350115","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T18:11:46Z","timestamp":1229019106000},"page":"115-123","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":44,"title":["Effects of tri-iodothyronine and insulin-like growth factor-I (IGF-I) on alkaline phosphatase activity, [3H]thymidine incorporation and IGF-I receptor mRNA in cultured rat epiphyseal chondrocytes"],"prefix":"10.1530","volume":"135","author":[{"given":"C.","family":"Ohlsson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Nilsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Isaksson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bentham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lindahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/135\/1\/joe_135_1_014.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/135\/1\/115\/101267\/joe_135_1_014.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/135\/1\/115\/101267\/joe_135_1_014.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:27:16Z","timestamp":1783607236000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/135\/1\/115\/17388\/Effects-of-tri-iodothyronine-and-insulin-like"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,10,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1350115","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1992,10,1]]}}}