{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:18:14Z","timestamp":1783516694943,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Microsome fractions prepared from the mammary glands of non-pregnant, pregnant and lactating sheep have been used to study binding of 125I-labelled transforming growth factor-\u03b1 (TGF-\u03b1). Binding was dependent on microsomal protein concentration, time and temperature. It showed the characteristics of an epidermal growth factor (EGF) receptor, being displaced by TGF-\u03b1 and EGF, but not by insulin or IGF-I. The non-linear curve fitting program LIGAND was used to determine affinity and number of binding sites. A single class of high-affinity binding sites was found. The apparent dissociation constant (Kd) was similar in all physiological states (2\u00b743\u00b10\u00b727 mol\/l \u00d7 10\u221210, n=23). Numbers of binding sites were lower in late-pregnant (20 weeks) and lactating sheep (14\u00b707\u00b1 2\u00b745 fmol\/mg protein, n=10) than in non-pregnant, 10-or 15-week pregnant sheep (43\u00b704\u00b15\u00b793 fmol\/mg protein, n=13).<\/jats:p>\n                  <jats:p>DNA synthesis by mammary alveolar epithelial cells cultured on collagen gels was increased twofold by TGF-a (maximum response at 10 \u03bcg\/l; 1\u00b78 nmol\/l) but not by EGF. Cells derived from 15- to 20-week pregnant sheep responded significantly to TGF-\u03b1 on day 3 of culture, but the response was delayed to day 4\u20135 of culture in cells from other physiological states. Dose\u2013response was not significantly affected. TGF-\u03b1 and IGF-I produced an additive effect on DNA synthesis. Oestradiol (10\u221212 to 10\u22129m), a potential stimulator of the TGF-\u03b1 gene, did not stimulate DNA synthesis alone, or in combination with IGF-I. It is concluded that growth factors acting via the EGF receptor play a role in ruminant mammary development, but whether they mediate oestradiol effects remains unresolved.<\/jats:p>\n                  <jats:p>Journal of Endocrinology (1995) 144, 165\u2013171<\/jats:p>","DOI":"10.1677\/joe.0.1440165","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:39:29Z","timestamp":1229017169000},"page":"165-171","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":11,"title":["Transforming growth factor-\u03b1: receptor binding and action on DNA synthesis in the sheep mammary gland"],"prefix":"10.1530","volume":"144","author":[{"given":"C D","family":"Moorby","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J A","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I A","family":"Forsyth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/144\/1\/joe_144_1_021.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/144\/1\/165\/103560\/joe_144_1_021.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/144\/1\/165\/103560\/joe_144_1_021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:47:28Z","timestamp":1783514848000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/144\/1\/165\/18157\/Transforming-growth-factor-receptor-binding-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1440165","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1995,1,1]]}}}