{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:22:22Z","timestamp":1783516942413,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Insulin secretion is regulated by neural and neurohormonal factors. The report of nerves releasing pituitary adenylate cyclase-activating polypeptide (PACAP) \u2013 a 38 amino acid peptide \u2013 in the endocrine pancreas, suggests it may be important in modulating insulin release. We therefore carried out receptor-binding studies on membranes prepared from the glucose-responsive clonal \u03b2-cell line HIT-T15, and also examined the effects of PACAP38, PACAP27 \u2013 a C-terminal truncated form of the peptide \u2013 and vasoactive intestinal peptide (VIP) on insulin and islet amyloid polypeptide (IAPP) release. We showed by chemical cross-linking that PACAP and VIP stimulate secretion from the clonal cells by binding to a receptor with a molecular weight of 67 kDa (n=4). Binding was saturable when membranes were incubated with 125I-PACAP27 (Kd 1\u00b72\u00b10\u00b72 nm; Bmax 415\u00b77\u00b135\u00b73 fmol\/mg; n=4) or 125I-VIP (Kd 1\u00b73\u00b10\u00b73 nm; Bmax 354\u00b78 \u00b1 42\u00b78 fmol\/mg; n=4). We also demonstrated an increase in glucose-stimulated insulin (PACAP27, 366\u00b76 \u00b125\u00b78% PACAP38, 389\u00b79 \u00b113\u00b74%; VIP, 342\u00b76\u00b1 16\u00b71% of control; all at 1 \u03bcm, P&amp;lt;0\u00b701 vs control) and IAPP release (PACAP27, 236\u00b79 \u00b126\u00b72%; PACAP38, 226\u00b75 \u00b1 10\u00b79%; VIP, 242\u00b79 \u00b1 15\u00b78% of control; all at 1 \u03bcm, P&amp;lt;0\u00b701 vs control). Incubation of the cells with these peptides, for a duration of 12 h, in the presence of 5\u00b75 mm glucose, did not alter the expression of insulin or IAPP. These findings suggest that PACAP and VIP stimulate secretion of insulin and IAPP by binding to a 67 kDa protein on clonal \u03b2-cells and do not alter the transcription of insulin and IAPP under the conditions tested.<\/jats:p>\n                  <jats:p>Journal of Endocrinology (1995) 147, 121\u2013130<\/jats:p>","DOI":"10.1677\/joe.0.1470121","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T17:39:29Z","timestamp":1229017169000},"page":"121-130","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":12,"title":["A 67 kDa protein mediates pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal peptide-stimulated insulin secretion in a hamster clonal \u03b2-cell line"],"prefix":"10.1530","volume":"147","author":[{"given":"R N","family":"Kulkarni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D M","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Ghatei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S R","family":"Bloom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/147\/1\/joe_147_1_015.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/147\/1\/121\/104265\/joe_147_1_015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/147\/1\/121\/104265\/joe_147_1_015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:58:37Z","timestamp":1783515517000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/147\/1\/121\/18398\/A-67-kDa-protein-mediates-pituitary-adenylate"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,10,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1470121","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1995,10,1]]}}}