{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T14:15:31Z","timestamp":1783520131324,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Endothelin-1 (ET-1) is present in ovine endometrium, primarily in epithelial cells, and increases around the time of implantation. We examined the cell type expressing ET-binding sites in vitro and whether ET-1 has mitogenic actions in the endometrium, alone or in synergy with other growth factors. Purified epithelial and stromal cells were prepared from luteal-phase endometrium. Specific receptors were demonstrated by binding of 125I-ET-1 and proliferative effects of ET-1 and\/or other growth factors determined by uptake of [3H]thymidine by cells in serum-free culture. 125I-ET-1 bound to both epithelial (2516 \u00b1 820 c.p.m.\/well) and stromal (6368 \u00b1 1350 c.p.m.\/well) cells and was displaced by ET-1 (1 \u03bcmol l\u22121). There were no proliferative effects of ET on epithelial cells. ET-1 (10 nmol l\u22121) stimulated uptake of [3H]thymidine by stromal cells under serum-free conditions in 13\/20 individual cell preparations, to 149 \u00b1 13% of control (untreated=100%) with dose-dependence between the range of 1 to 100 nmol l\u22121. Stimulation by fetal calf serum was to 377 \u00b1 126% of control. The effects on proliferation by other growth factors (dose; % of control \u00b1 s.e.m., number of positives\/total number of cell preparations) were: IGF-I (13 nmol l\u22121; 182 \u00b1 14, 4\/4), epidermal growth factor (EGF; 4\u00b78 nmol l\u22121; 132 \u00b1 5%, 7\/7), platelet-derived growth factor-BB (0\u00b74 nmol l\u22121; 146 \u00b1 3, 2\/2) and leukaemia inhibitory factor (0\u00b74 nmol l\u22121; 110 \u00b1 2, 3\/3). All stimulations except that of EGF were significant and dose-responsive but only insulin was additive with ET (350 \u00b1 35, 5\/5). ET-1 also stimulated expression of the the AP-1 cis element c-jun, this being maximal at 60 min of exposure to mitogen. ET-1, along with other growth factors has a likely paracrine role in cellular proliferation in the endometrium, possibly in association with blastocyst implantation.<\/jats:p>\n                  <jats:p>Journal of Endocrinology (1997) 152, 283\u2013290<\/jats:p>","DOI":"10.1677\/joe.0.1520283","type":"journal-article","created":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T18:42:13Z","timestamp":1102012933000},"page":"283-290","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":13,"title":["Mitogenic actions of endothelin and other growth factors in ovine endometrium"],"prefix":"10.1530","volume":"152","author":[{"given":"L A","family":"Salamonsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R J","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J K","family":"Findlay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/152\/2\/joe_152_2_015.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/152\/2\/283\/105511\/joe_152_2_015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/152\/2\/283\/105511\/joe_152_2_015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:17:27Z","timestamp":1783516647000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/152\/2\/283\/18809\/Mitogenic-actions-of-endothelin-and-other-growth"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,2,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1520283","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[1997,2,1]]}}}