{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T17:13:16Z","timestamp":1783530796034,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study was conducted to evaluate the responsiveness of human nonpregnant myometrium to endothelin 1 (ET1) (10(-10) M-10(-6 )M) and KCl (80 mM) in relation to the hormonal profile of the women, who were allocated into three groups: group 1, premenopausal follicular phase, n=14, group 2, premenopausal luteal phase, n=20, and group 3, postmenopausal women, n=12. At a concentration of 10(-6 )M, ET1 in both groups 1 and 2 induced very low ripples of high frequency (group 1: 80+\/-14%, n=5, group 2: 314+\/-63%, n=11; P&amp;lt;0.05 compared with the pretreatment frequency) which lasted significantly longer in group 2 (29+\/-2 min, n=10, P&amp;lt;0.05) than in group 1 (20+\/-2 min, n=5), increasing the basal tone (group 1: 57.9+\/-6%, n=5, group 2: 64.4+\/-4%, n=6), the amplitude of myometrial contractility (group 1: 1.2+\/-0.07 g, n=5, group 2: 1.6+\/-0.1 g, n=7, P&amp;lt;0.05) and the area under the contractility curve (AUC; group 1: 8.4+\/-1.1 gxmin, n=6, group 2: 11.9+\/-1.6 g x min, n=11). In group 3, ET1 (10(-6 )M) created a sustained long-lasting contraction (initial phase: 43+\/-6 min, n=6) characterized by the complete obliteration of spontaneous contractility with no ripples at all, and increasing significantly (P&amp;lt;0.05) the amplitude of myometrial contractility (2.8+\/-0.5 g, n=6), the AUC (24.7+\/-3.3 g x min, n=6), as well as the basal tone (183.6+\/-21%, n=6) compared with the two premenopausal groups. In all three groups KCl exposure induced an initial rise (mean amplitude value: 1.1 g) followed by a relaxation phase to the primal baseline level (mean duration value: 12 min). Addition of ET1 (10(-6 )M) to KCl (80 mM) induced a similar pattern of contractility to that evoked by ET1 alone which, compared with KCl alone lasted significantly longer (P&amp;lt;0.05) in all three groups (group 1: 20+\/-2 min, n=6; group 2: 23+\/-2 min, n=6; group 3: 35+\/-3 min, n=5). In group 3, the percentage change in basal tone was significantly smaller following KCl than after the combination of KCl plus ET1 (149+\/-16%, n=5; P&amp;lt;0.01), indicating a different mechanism of contractility between KCl and ET1. These results demonstrate for the first time differences in myometrial response to ET1 between pre- and postmenopausal women. It is suggested that KCl and ET1 affect uterine contractility through different mechanisms and that ovarian steroids may play a regulatory role in human uterine responsiveness to ET1.<\/jats:p>","DOI":"10.1677\/joe.0.1680153","type":"journal-article","created":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T18:42:13Z","timestamp":1102012933000},"page":"153-162","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":4,"title":["In vitro effects of endothelin-1 on the contractility of myometrium obtained from pre- and postmenopausal women"],"prefix":"10.1530","volume":"168","author":[{"given":"E","family":"Domali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Asprodini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PA","family":"Molyvdas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IE","family":"Messinis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/168\/1\/153.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/168\/1\/153\/108667\/153.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/168\/1\/153\/108667\/153.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:14:26Z","timestamp":1783527266000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/168\/1\/153\/19853\/In-vitro-effects-of-endothelin-1-on-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,1,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.0.1680153","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[2001,1,1]]}}}