{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:41Z","timestamp":1784557181088,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,10]]},"abstract":"<jats:p>Tumor necrosis factor-\u03b1 (TNF\u03b1) may play a role in the development of autoimmune thyroiditis such as Hashimoto\u2019s thyroiditis. In the present study, we examined whether TNF\u03b1 induced its own expression in FRTL-5 rat thyroid cells. Lipopolysaccharide (LPS) markedly increased TNF\u03b1 mRNA levels in FRTL-5 cells as assessed by semiquantitative RT-PCR. In addition, LPS-stimulated cells released TNF\u03b1 protein into the culture medium. Similarly, TNF\u03b1 induced its own gene and protein expression in FRTL-5 cells as assessed by RT-PCR and metabolic labeling and immunoprecipitation of TNF\u03b1. The autoinduction of TNF\u03b1 gene was also observed in TNF\u03b1-stimulated human thyroid epithelial cells. TNF\u03b1 induction was specific to LPS and TNF\u03b1 since interferon-\u03b1 or amiodarone failed to increase TNF\u03b1 mRNA levels in FRTL-5 cells. Human TNF\u03b1 induced rat TNF\u03b1 gene expression, indicating that type 1 TNF receptor (TNF-R) is involved in the autoinduction. TNF\u03b1 did not increase either type 1 or type 2 TNF-R mRNA levels, suggesting that upregulation of TNF receptors is not involved in the autoinduction of TNF\u03b1. Although the biological significance of autoinduction of TNF\u03b1 remains unclear, our results suggest that thyroid epithelial cells may participate in the development of autoimmune thyroiditis through production of TNF\u03b1. Furthermore, inhibition of TNF\u03b1 production in the thyroid may represent a novel approach to mitigating inflammation in autoimmune thyroiditis.<\/jats:p>","DOI":"10.1677\/joe.1.05887","type":"journal-article","created":{"date-parts":[[2005,10,7]],"date-time":"2005-10-07T13:42:55Z","timestamp":1128692575000},"page":"17-24","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":10,"title":["Autoinduction of tumor necrosis factor-\u03b1 in FRTL-5 rat thyroid cells"],"prefix":"10.1530","volume":"187","author":[{"given":"Kouki","family":"Mori","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumi","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayumi","family":"Komatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-ichi","family":"Tani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeko","family":"Hoshikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadayoshi","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/187\/1\/1870017.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/187\/1\/1870017.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:55:34Z","timestamp":1784555734000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/187\/1\/17-24\/20963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,10]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1677\/joe.1.05887","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,10]]}}}