{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:41Z","timestamp":1784557181480,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p>\n                    Controversy remains regarding whether the seco-steroid hormone 1,25-dihydroxyvitamin D\n                    <jats:sub>3<\/jats:sub>\n                    (1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    ) enhances calcium and phosphate movement across the intestinal epithelial cell by facilitated diffusion or a vesicular transport mechanism. In this study we investigated whether membrane trafficking, as judged by confocal microscopy, was sufficiently rapid in comparison to hormone-stimulated uptake of phosphate (\n                    <jats:sup>32<\/jats:sup>\n                    P). Primary cultures of chick intestinal cells were established overnight either in Petri dishes (uptake studies) or chambered coverslips (confocal microscopy). Addition of 130 pM 1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    resulted in an apparent increase in\n                    <jats:sup>32<\/jats:sup>\n                    P uptake within 1 min, relative to controls, that was statistically significant from 3\u201310 min of incubation. Using the endocytic marker dye, FM1\u201343, confocal microscopy revealed a profound decrease in membrane-associated fluorescence (apical&gt; basal) within 10 s of hormone treatment, a return of fluorescence at 15\u201365 s, followed by another round of decreasing and increasing fluorescence. Between 3\u20139 min of incubation, fluorescence intensity increased 50% (apical region) and 20% (basal region) over control conditions. An antibody (Ab 099) directed against a putative membrane receptor for 1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    (1,25D\n                    <jats:sub>3<\/jats:sub>\n                    -MARRS) inhibited both\n                    <jats:sup>32<\/jats:sup>\n                    P uptake, and changes in fluorescence. In addition, the protein kinase C (PKC) inhibitor, calphostin C, inhibited both\n                    <jats:sup>32<\/jats:sup>\n                    P uptake and the observed 1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    -mediated changes in fluorescence. At the microscopic level, calphostin C pretreatment abolished the very rapid redistribution of the endocytic marker dye, although a slight increase in fluorescence was still observed. We conclude that 1,25(OH)\n                    <jats:sub>2<\/jats:sub>\n                    D\n                    <jats:sub>3<\/jats:sub>\n                    -stimulated vesicular trafficking is mediated by the 1,25D\n                    <jats:sub>3<\/jats:sub>\n                    -MARRS protein, implicates a PKC signaling mechanism, and occurs in a time frame that is commensurate with a role in ion transport.\n                  <\/jats:p>","DOI":"10.1677\/joe.1.05945","type":"journal-article","created":{"date-parts":[[2005,4,7]],"date-time":"2005-04-07T15:42:47Z","timestamp":1112888567000},"page":"81-91","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":21,"title":["1,25-dihydroxyvitamin D3 stimulates vesicular transport within 5 s in polarized intestinal epithelial cells"],"prefix":"10.1530","volume":"185","author":[{"given":"Tremaine M","family":"Sterling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilka","family":"Nemere","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/185\/1\/1850081.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/185\/1\/1850081.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:55:39Z","timestamp":1784555739000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/185\/1\/81-91\/20884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1677\/joe.1.05945","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4]]}}}