{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T14:19:58Z","timestamp":1784557198424,"version":"3.55.0"},"reference-count":0,"publisher":"Bioscientifica","issue":"1","content-domain":{"domain":["joe.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>\n                    Differentiation of uterine stromal cells is critical for the establishment of pregnancy. This study had two purposes: (i) to validate the use of the UIII rat uterine stromal cell model for investigating mechanisms underlying decidual cell differentiation, and (ii) to use this cell model to identify a molecular switch for cellular entry into the decidual cell differentiation pathway. Quiescent rat uterine stromal cells were transfected with a 500 bp segment of the decidual prolactin-related protein (dPRP) promoter ligated to a luciferase reporter gene. Cells were incubated in low-serum medium, or in low-serum medium containing progesterone (1 \u03bcM), estradiol 17-\u03b2 (10 nM), cholera toxin (10 ng\/ml) and interleukin-11 (10 ng\/ml). Protein extracts were collected 48 h later and luciferase was measured in the cellular lysates. Cholera toxin and interleukin-11 stimulated luciferase expression (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) and addition of sex steroids further increased (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) dPRP promoter activity. Stromal cells did not proliferate (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) under differentiation conditions. Deletion analysis of the dPRP promoter revealed maximal luciferase expression between \u2212250 and \u2212500 bp relative to the transcription start site. Comparison of cyclin E\/Cdk2 activity between proliferating and differentiating cells showed a 3-fold increase (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) at 12 h in differentiating cells. The results suggest that cyclin E\/Cdk2 serves as a molecular switch for uterine stromal cell entry into the decidual cell differentiation pathway.\n                  <\/jats:p>","DOI":"10.1677\/joe.1.05957","type":"journal-article","created":{"date-parts":[[2005,1,21]],"date-time":"2005-01-21T16:17:56Z","timestamp":1106324276000},"page":"119-127","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":3,"title":["Stimulation of a rat uterine stromal cell line in culture reveals a molecular switch for endocrine-dependent differentiation"],"prefix":"10.1530","volume":"184","author":[{"given":"V","family":"Rider","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Potapova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","container-title":["Journal of Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/184\/1\/1840119.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/joe.bioscientifica.com\/downloadpdf\/journals\/joe\/184\/1\/1840119.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:55:40Z","timestamp":1784555740000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.bioscientifica.com\/joe\/article\/184\/1\/119-127\/20785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":0,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1677\/joe.1.05957","relation":{},"ISSN":["0022-0795","1479-6805"],"issn-type":[{"value":"0022-0795","type":"print"},{"value":"1479-6805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]}}}