{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T14:20:13Z","timestamp":1783520413457,"version":"3.55.0"},"reference-count":35,"publisher":"Bioscientifica","issue":"2","content-domain":{"domain":["journals.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the present study, we compared rat uterine contractility and myometrial inositol phosphate (InsP) production in response to activation of muscarinic and oxytocin receptors during pregnancy and at term. The level of myometrial phospholipase (PL) C\u03b2 was also determined by Western blotting at different stages of pregnancy and following administration of oestradiol, progesterone or vehicle. The results showed an increased potency of carbachol (CCh), a cholinergic muscarinic agonist, and oxytocin (OT) to enhance myometrial InsP production at term. This correlated with an increased potency of both agonists to induce contraction of the circular but not the longitudinal muscle. For both InsP production and contractile activity, the maximal response of CCh was unaltered, while that of OT was significantly increased. Interestingly, the increased responsiveness to CCh and OT was associated with an up-regulation of PLC\u03b21 and PLC\u03b23 enzymes. Such regulation is under the control of oestradiol since administration of this steroid to pregnant rats increased the amount of both enzymes by 200\u2013260%. In contrast, progesterone administration was without effect. The present study presents the first evidence that the expression of rat myometrial PLC\u03b21 and PLC\u03b23 is under the positive control of oestradiol. This could participate in the enhancement of myometrial InsP accumulation and uterine contraction at term in response to CCh and OT. Based on contraction studies, we also propose that the longitudinal and circular uterine muscles differ in the regulation of the PLC pathway during pregnancy.<\/jats:p>","DOI":"10.1677\/joe.1.06388","type":"journal-article","created":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T15:07:56Z","timestamp":1132240076000},"page":"197-204","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-5","source":"Crossref","is-referenced-by-count":6,"title":["Up-regulation of rat myometrial phospholipases C\u03b21 and C\u03b23 correlates with increased term sensitivity to carbachol and oxytocin"],"prefix":"10.1530","volume":"187","author":[{"given":"E","family":"Houdeau","sequence":"first","affiliation":[{"name":"INRA, 31931 Toulouse cedex 9, France 1","department":["Unite\u0301 de Neuro-Gastroente\u0301rologie et Nutrition"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Le\u0301vy","sequence":"additional","affiliation":[{"name":"UMR-CNRS 7079, Paris cedex 05, France","department":["Laboratoire de Physiologie et Physiopathologie"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Mhaouty-Kodja","sequence":"additional","affiliation":[{"name":"UMR-CNRS 7079, Paris cedex 05, France","department":["Laboratoire de Physiologie et Physiopathologie"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"416","reference":[{"key":"2026070809450796400_ABDALLA-ETAL-2000","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0303-7207(99)00259-2","volume":"160","author":"Abdalla","year":"2000","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026070809450796400_ABDALLA-ETAL-2004","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.mce.2003.10.040","volume":"213","author":"Abdalla","year":"2004","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026070809450796400_BRADFORD-1976","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"Bradford","year":"1976","journal-title":"Anaytical Biochemistry"},{"key":"2026070809450796400_CHOPPIN-ETAL-1999","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1038\/sj.bjp.0702696","volume":"127","author":"Choppin","year":"1999","journal-title":"British Journal of Pharmacology"},{"key":"2026070809450796400_COHEN-TANNOUDJI-ETAL-1991","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1677\/jme.0.0060137","volume":"6","author":"Cohen-Tannoudji","year":"1991","journal-title":"Journal of Molecular Endocrinology"},{"key":"2026070809450796400_COHEN-TANNOUDJI-ETAL-1995","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1095\/biolreprod53.1.55","volume":"53","author":"Cohen-Tannoudji","year":"1995","journal-title":"Biology of Reproduction"},{"key":"2026070809450796400_CUEILLE-ETAL-2003","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0960-0760(03)00271-1","volume":"86","author":"Cueille","year":"2003","journal-title":"Journal of Steroid Biochemistry and Molecular Biology"},{"key":"2026070809450796400_DODGE-ETAL-1999","first-page":"1977","volume":"13","author":"Dodge","year":"1999","journal-title":"Molecular Endocrinology"},{"key":"2026070809450796400_DWIVEDI-AND-PANDEY-1999","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1046\/j.1471-4159.1999.0730780.x","volume":"73","author":"Dwivedi","year":"1999","journal-title":"Journal of Neurochemistry"},{"key":"2026070809450796400_EL-ALJ-ETAL-1993","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1016\/S0022-3565(25)38265-0","volume":"265","author":"El Alj","year":"1993","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"2026070809450796400_ELWARDY-MEREZAK-ETAL-1994","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1677\/jme.0.0130023","volume":"13","author":"Elwardy-Me\u0301re\u0301zak","year":"1994","journal-title":"Journal of Molecular Endocrinology"},{"key":"2026070809450796400_GOUREAU-ETAL-1992","doi-asserted-by":"crossref","first-page":"C257","DOI":"10.1152\/ajpcell.1992.263.1.C257","volume":"263","author":"Goureau","year":"1992","journal-title":"American Journal of Physiology \u2013 Cell Physiology"},{"key":"2026070809450796400_HOUDEAU-ETAL-2003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S1566-0702(02)00233-3","volume":"104","author":"Houdeau","year":"2003","journal-title":"Autonomic Neuroscience"},{"key":"2026070809450796400_KANG-ETAL-1997","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0378-1119(97)00213-8","volume":"197","author":"Kang","year":"1997","journal-title":"Gene"},{"key":"2026070809450796400_KHAC-ETAL-1996","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0022-3565(25)12241-6","volume":"276","author":"Khac","year":"1996","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"2026070809450796400_KIM-ETAL-1997","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/38508","volume":"389","author":"Kim","year":"1997","journal-title":"Nature"},{"key":"2026070809450796400_KITAZAWA-ETAL-2001","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/S0196-9781(01)00402-8","volume":"22","author":"Kitazawa","year":"2001","journal-title":"Peptides"},{"key":"2026070809450796400_KLINGE-2001","doi-asserted-by":"crossref","first-page":"2905","DOI":"10.1093\/nar\/29.14.2905","volume":"29","author":"Klinge","year":"2001","journal-title":"Nucleic Acids Research"},{"key":"2026070809450796400_LAJAT-ETAL-1996","doi-asserted-by":"crossref","first-page":"C895","DOI":"10.1152\/ajpcell.1996.271.3.C895","volume":"271","author":"Lajat","year":"1996","journal-title":"American Journal of Physiology \u2013 Cell Physiology"},{"key":"2026070809450796400_LARCHER-ETAL-1995","doi-asserted-by":"crossref","first-page":"5350","DOI":"10.1210\/endo.136.12.7588281","volume":"136","author":"Larcher","year":"1995","journal-title":"Endocrinology"},{"key":"2026070809450796400_LEFEBVRE-ETAL-1995","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1071\/RD9950603","volume":"7","author":"Lefebvre","year":"1995","journal-title":"Reproduction, Fertility and Development"},{"key":"2026070809450796400_LEGRAND-ETAL-1991","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/S0022-3565(25)23110-X","volume":"256","author":"Legrand","year":"1991","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"2026070809450796400_LIMON-BOULEZ-ETAL-1997","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1095\/biolreprod57.5.1175","volume":"57","author":"Limon-Boulez","year":"1997","journal-title":"Biology of Reproduction"},{"key":"2026070809450796400_MHAOUTY-KODJA-ETAL-2001","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1111\/j.1469-7793.2001.00123.x","volume":"536","author":"Mhaouty-Kodja","year":"2001","journal-title":"Journal of Physiology"},{"key":"2026070809450796400_MHAOUTY-KODJA-ETAL-2004","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1095\/biolreprod.103.021535","volume":"70","author":"Mhaouty-Kodja","year":"2004","journal-title":"Biology of Reproduction"},{"key":"2026070809450796400_OU-ETAL-2000","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/S0002-9378(00)70347-4","volume":"182","author":"Ou","year":"2000","journal-title":"American Journal of Obstetrics and Gynecology"},{"key":"2026070809450796400_PERUZZI-ETAL-2002","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/S1388-1981(02)00269-X","volume":"1584","author":"Peruzzi","year":"2002","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026070809450796400_PHANEUF-ETAL-1995","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1530\/jrf.0.1030121","volume":"103","author":"Phaneuf","year":"1995","journal-title":"Reproduction, Fertility and Development"},{"key":"2026070809450796400_PIIPER-ETAL-1997","doi-asserted-by":"crossref","first-page":"G135","DOI":"10.1152\/ajpgi.1997.272.1.G135","volume":"272","author":"Piiper","year":"1997","journal-title":"American Journal of Physiology \u2013 Gastrointestinal and Liver Physiology"},{"key":"2026070809450796400_REBECCHI-AND-PENTYALA-2000","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1152\/physrev.2000.80.4.1291","volume":"80","author":"Rebecchi","year":"2000","journal-title":"Physiological Reviews"},{"key":"2026070809450796400_RIEMER-ETAL-1986","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1210\/endo-119-2-699","volume":"119","author":"Riemer","year":"1986","journal-title":"Endocrinology"},{"key":"2026070809450796400_RIEMER-ETAL-1987","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S0026-895X(25)13052-6","volume":"32","author":"Riemer","year":"1987","journal-title":"Molecular Pharmacology"},{"key":"2026070809450796400_STRASSHEIM-AND-WILLIAMS-2000","doi-asserted-by":"crossref","first-page":"39767","DOI":"10.1074\/jbc.M007775200","volume":"275","author":"Strassheim","year":"2000","journal-title":"Journal of Biological Chemistry"},{"key":"2026070809450796400_VIVAT-ETAL-1992","doi-asserted-by":"crossref","first-page":"7975","DOI":"10.1016\/S0021-9258(18)42394-0","volume":"267","author":"Vivat","year":"1992","journal-title":"Journal of Biological Chemistry"},{"key":"2026070809450796400_WU-ETAL-1997","doi-asserted-by":"crossref","first-page":"4005","DOI":"10.1210\/endo.138.9.5394","volume":"138","author":"Wu","year":"1997","journal-title":"Endocrinology"}],"container-title":["Journal of Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/joe.bioscientifica.com\/view\/journals\/joe\/187\/2\/1870197.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/187\/2\/197\/113160\/1870197.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article-pdf\/187\/2\/197\/113160\/1870197.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T13:45:16Z","timestamp":1783518316000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.bioscientifica.com\/joe\/article\/187\/2\/197\/20950\/Up-regulation-of-rat-myometrial-phospholipases-C-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,1]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,11,1]]}},"URL":"https:\/\/doi.org\/10.1677\/joe.1.06388","relation":{},"ISSN":["1479-6805","0022-0795"],"issn-type":[{"value":"1479-6805","type":"electronic"},{"value":"0022-0795","type":"print"}],"subject":[],"published":{"date-parts":[[2005,11,1]]}}}