{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:50:56Z","timestamp":1792788656664,"version":"4.5.0"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82270741"],"award-info":[{"award-number":["82270741"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"crossref","award":["LY22H050001"],"award-info":[{"award-number":["LY22H050001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017623","name":"Zhejiang Provincial Outstanding Youth Science Foundation","doi-asserted-by":"crossref","award":["LR24H200001"],"award-info":[{"award-number":["LR24H200001"]}],"id":[{"id":"10.13039\/501100017623","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Central Government Funds for Guiding Local Science and Technology Development","award":["2024ZY01007"],"award-info":[{"award-number":["2024ZY01007"]}]},{"name":"the National Key Research and Development Program","award":["2022YFC2705103"],"award-info":[{"award-number":["2022YFC2705103"]}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["JASN"],"published-print":{"date-parts":[[2025,3]]},"abstract":"\n Key Points<\/jats:title>\n \n \n \n Serum anti-proteasome subunit alpha type 1 (PSMA1) autoantibodies were specifically elevated in the active phase of idiopathic nephrotic syndrome, which may assist in disease diagnosis and monitoring.<\/jats:p>\n <\/jats:list-item>\n \n Serum anti-PSMA1 antibodies could cause damage to the glomerular filtration barrier, which may be a pathogenic antibody of idiopathic nephrotic syndrome.<\/jats:p>\n <\/jats:list-item>\n \n PSMA1 played an important role in the maintenance of podocyte morphology and function.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Background<\/jats:title>\n The antibody against proteasome subunit alpha type 1 (PSMA1) is a podocyte autoantibody in children with idiopathic nephrotic syndrome identified in our previous study. The aim of this study was to explore the characteristics of idiopathic nephrotic syndrome in children and the mechanism underlying its involvement in the development of idiopathic nephrotic syndrome.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The levels of serum anti-PSMA1 autoantibodies in children were detected through protein microarray and compared among different disease groups. The recombinant PSMA1 protein was injected subcutaneously and intraperitoneally into mice to observe glomerular morphology and function. The PSMA1-knockdown and PSMA1-overexpressing cell lines were constructed from mouse podocytes, and their cytoskeleton and function were analyzed. Homozygous zebrafish with\n psma1<\/jats:italic>\n knockout were observed.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The levels of serum anti-PSMA1 autoantibodies were higher in children with idiopathic nephrotic syndrome and varied with urinary protein. In mice immunized with PSMA1, the presence of serum anti-PSMA1 autoantibody caused albuminuria and damage to the glomerular filtration membrane. Deficiency of PSMA1 impaired the podocyte cytoskeleton and physiological function. Complete deletion of\n psma1<\/jats:italic>\n caused edema, abnormal glomerular morphology, and effacement of foot processes in zebrafish.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n PSMA1 played an important role in the maintenance of podocyte morphology and function.<\/jats:p>\n <\/jats:sec>","DOI":"10.1681\/asn.0000000525","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T12:14:03Z","timestamp":1728476043000},"page":"406-419","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Autoantibodies Targeting Proteasome Subunit Alpha Type 1 in Autoimmune Podocytopathies"],"prefix":"10.1681","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8121-7062","authenticated-orcid":false,"given":"Huihui","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9554-3572","authenticated-orcid":false,"given":"Dongjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0335-6729","authenticated-orcid":false,"given":"Hanyan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4503-2331","authenticated-orcid":false,"given":"Shifan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5237-7822","authenticated-orcid":false,"given":"Jiayu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6076-3806","authenticated-orcid":false,"given":"Jianhua","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,10,9]]},"reference":[{"issue":"10404","key":"B1-20250303","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/S0140-6736(23)01051-6","article-title":"Childhood nephrotic syndrome","volume":"402","author":"Vivarelli","year":"2023","journal-title":"Lancet."},{"issue":"1","key":"B2-20250303","doi-asserted-by":"crossref","first-page":"e2020029249","DOI":"10.1542\/peds.2020-029249","article-title":"Incidence and relapse of idiopathic nephrotic syndrome: 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