{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:06:44Z","timestamp":1786133204789,"version":"build-2736575974"},"reference-count":51,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01-DK098410"],"award-info":[{"award-number":["R01-DK098410"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01-DK120908"],"award-info":[{"award-number":["R01-DK120908"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01-DK115968"],"award-info":[{"award-number":["R01-DK115968"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"crossref","award":["R01-DK115968"],"award-info":[{"award-number":["R01-DK115968"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["JASN"],"published-print":{"date-parts":[[2025,3]]},"abstract":"\n Key Points<\/jats:title>\n \n \n \n Piezo channels, known for detecting mechanical pressure, were found to be expressed at the lacuna channel membranes of nephrocytes.<\/jats:p>\n <\/jats:list-item>\n \n Piezo loss of function caused nephrocyte dysfunction, including disrupted slit diaphragm structure and altered lacuna channel morphology.<\/jats:p>\n <\/jats:list-item>\n \n Piezo deficiency led to internalized slit diaphragm proteins, reduced autophagy, increased endoplasmic reticulum stress, and impaired calcium homeostasis.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Background<\/jats:title>\n \n The\n Piezo<\/jats:italic>\n gene encodes a highly conserved cell membrane protein responsible for sensing pressure. The glomerular kidney and the slit diaphragm filtration structure depend on pressure for filtration. However, how Piezo is involved in kidney function and in maintaining the slit diaphragm filtration structure is not clear.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We used\n Drosophila<\/jats:italic>\n pericardial nephrocytes, filtration kidney cells with striking structural and functional similarities to human podocytes, in a loss-of-function model (mutant and knockdown) to study the roles of Piezo in nephrocyte filtration and function.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Piezo<\/jats:italic>\n was highly expressed at the invaginated membranes (lacuna channels) of nephrocytes. A\n Piezo<\/jats:italic>\n loss-of-function mutant showed significant nephrocyte functional decline. Nephrocyte-specific silencing of Piezo showed disruption of the slit diaphragm filtration structure and significant functional defects. Electron microscopy showed that silencing Piezo in nephrocytes led to reduced slit diaphragm density and abnormal shape of lacuna channels. Moreover, the Piezo-deficient nephrocytes showed internalized slit diaphragm component proteins, reduced autophagy, increased endoplasmic reticulum stress, and reduced calcium influx.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Together, our findings suggest that Piezo plays an important role in the calcium homeostasis of nephrocytes and is required for maintaining nephrocyte function and the slit diaphragm filtration structure.<\/jats:p>\n <\/jats:sec>","DOI":"10.1681\/asn.0000000529","type":"journal-article","created":{"date-parts":[[2024,10,17]],"date-time":"2024-10-17T12:11:56Z","timestamp":1729167116000},"page":"393-405","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":6,"title":["Piezo, Nephrocyte Function, and Slit Diaphragm Maintenance in Drosophila"],"prefix":"10.1681","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7942-3406","authenticated-orcid":false,"given":"Yunpo","family":"Zhao","sequence":"first","affiliation":[{"name":"Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"},{"name":"Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7347-4774","authenticated-orcid":false,"given":"Jianli","family":"Duan","sequence":"additional","affiliation":[{"name":"Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"},{"name":"Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6538-0177","authenticated-orcid":false,"given":"Iden D.","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Child Health Research Center, School of Medicine, University of Virginia, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1903-7295","authenticated-orcid":false,"given":"Joyce","family":"van de Leemput","sequence":"additional","affiliation":[{"name":"Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"},{"name":"Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio E.","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Child Health Research Center, School of Medicine, University of Virginia, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5177-7798","authenticated-orcid":false,"given":"Zhe","family":"Han","sequence":"additional","affiliation":[{"name":"Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"},{"name":"Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2024,10,17]]},"reference":[{"issue":"1","key":"B1-20250303","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0002-9629(15)40679-2","article-title":"Physiology of renal sodium 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