{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:45:45Z","timestamp":1786135545523,"version":"build-2736575974"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["HE 7456\/4-1"],"award-info":[{"award-number":["HE 7456\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["HE 7456\/7-1"],"award-info":[{"award-number":["HE 7456\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["HE 7456\/6-1"],"award-info":[{"award-number":["HE 7456\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["431984000"],"award-info":[{"award-number":["431984000"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Berta-Ottenstein-Programme of the Faculty of Medicine, University of Freiburg"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["JASN"],"published-print":{"date-parts":[[2025,8]]},"abstract":"\n Key Points<\/jats:title>\n \n \n \n \n We developed a fast assay testing\n Drosophila<\/jats:italic>\n nephrocyte function, suitable for whole-animal drug screening in these podocyte-like cells.\n <\/jats:p>\n <\/jats:list-item>\n \n \n Rac1<\/jats:italic>\n overexpression disrupted nephrocyte slit diaphragm architecture, providing a screening background relevant to podocyte (patho)biology.\n <\/jats:p>\n <\/jats:list-item>\n \n A pilot screen using the novel podocytopathy platform identified that zacopride restores slit diaphragms despite excess of Rac1.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>\n \n Background<\/jats:title>\n \n The complex cellular architecture of the glomerular filtration barrier is not recapitulated\n in vitro<\/jats:italic>\n , representing a major obstacle for drug screening. This contributes significantly to a therapeutic gap for the heterogeneous diseases affecting the podocyte. Phenotypic drug screening using whole organisms is inherently slow but can reveal entirely unexpected therapies that are unattainable by conventional screening. The\n Drosophila<\/jats:italic>\n nephrocyte features a molecularly conserved filtration barrier, suitable for whole-animal screening in podocytopathies.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We generated transgenic\n Drosophila<\/jats:italic>\n expressing a secretable variant of green fluorescent protein as a genetically encoded tracer for rapid analysis of nephrocyte function. Animals were exposed to drugs in liquid food before recording nephrocyte fluorescence intensity. The slit diaphragm architecture was investigated using immunofluorescence and subsequent automated quantification.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The genetically encoded tracer, combined with fast detection through enhanced widefield fluorescence microscopy, provided a faster but reliable screening assay for nephrocyte function compared with the established approach using FITC-albumin\n ex vivo<\/jats:italic>\n . Rac1 is a key regulator of the actin cytoskeleton, involved in maintaining podocyte structure and function. In nephrocytes, overexpression of\n Rac1<\/jats:italic>\n resulted in mislocalization of slit diaphragm proteins and deeper membrane invaginations. Since nephrocyte function was further decreased as detected by the novel assay, we used this screening background relevant to podocyte biology for a pilot screen of 100 drugs. We identified significant improvement of nephrocyte function for zacopride, a respective agonist or antagonist of serotonin receptors, which restored the slit diaphragm architecture despite overexpression of\n Rac1<\/jats:italic>\n . The mechanism of action of zacopride appeared independent from the orthologs of the mammalian target proteins or direct Rac1 inhibition, suggesting a pleiotropic target. This hit from the pilot screen illustrates the potential of phenotypic drug screening to reveal unexpected therapeutic options.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n We present a proof of concept for whole-animal drug screening using podocyte-like\n Drosophila<\/jats:italic>\n nephrocytes.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1681\/asn.0000000677","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:27:04Z","timestamp":1743121624000},"page":"1505-1519","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["A Drug Screening Platform for Podocytopathies in Drosophila Nephrocytes"],"prefix":"10.1681","volume":"36","author":[{"given":"Dominik","family":"Spitz","sequence":"first","affiliation":[{"name":"Renal Division, Department of Medicine, Faculty of Medicine and Medical Center - University of Freiburg, Freiburg, 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Studies, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3845-3489","authenticated-orcid":false,"given":"Ronen","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0441-7749","authenticated-orcid":false,"given":"Tobias","family":"Hermle","sequence":"additional","affiliation":[{"name":"Renal Division, Department of Medicine, Faculty of Medicine and Medical Center - University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"issue":"1","key":"B1-20251209","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.kisu.2021.11.003","article-title":"Epidemiology of chronic 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