{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:03:23Z","timestamp":1786133003229,"version":"build-2736575974"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>\n                    Generating kidney organoids using human stem cells could offer promising prospects for research and therapeutic purposes. However, no cell-based strategy has generated nephrons displaying an intact three-dimensional epithelial filtering barrier. Here, we generated organoids using murine embryonic kidney cells, and documented that these tissues recapitulated the complex three-dimensional filtering structure of glomerular slits\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    and accomplished selective glomerular filtration and tubular reabsorption. Exploiting this technology, we mixed human amniotic fluid stem cells with mouse embryonic kidney cells to establish three-dimensional chimeric organoids that engrafted\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    and grew to form vascularized glomeruli and tubular structures. Human cells contributed to the formation of glomerular structures, differentiated into podocytes with slit diaphragms, and internalized exogenously infused BSA, thus attaining\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    degrees of specialization and function unprecedented for donor stem cells. In conclusion, human amniotic fluid stem cell chimeric organoids may offer new paths for studying renal development and human podocyte disease, and for facilitating drug discovery and translational research.\n                  <\/jats:p>","DOI":"10.1681\/asn.2015030316","type":"journal-article","created":{"date-parts":[[2015,10,29]],"date-time":"2015-10-29T23:08:06Z","timestamp":1446160086000},"page":"1400-1411","source":"Crossref","is-referenced-by-count":48,"title":["Functional Human Podocytes Generated in Organoids from Amniotic Fluid Stem Cells"],"prefix":"10.1681","volume":"27","author":[{"given":"Christodoulos","family":"Xinaris","sequence":"first","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Benedetti","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rubina","family":"Novelli","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Abbate","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Rizzo","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Conti","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Tomasoni","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Corna","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Pozzobon","sequence":"additional","affiliation":[{"name":"Stem Cells and Regenerative Medicine Laboratory, Foundation Institute of Pediatric Research Fondazione Citt\u00e0 della Speranza, Padua, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Cavallotti","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, Bergamo, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yokoo","sequence":"additional","affiliation":[{"name":"The Jikei University School of Medicine, Tokyo, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Morigi","sequence":"additional","affiliation":[{"name":"IRCCS-Istituto di Ricerche Farmacologiche \u2018Mario Negri\u2019, Centro 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Quantitative analysis of tubular passage of FITC-dextrans in kidneys of rats.","volume":"369","author":"Steinhausen","year":"1977","journal-title":"Pflugers Arch"},{"key":"B42-20230819","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.2215\/CJN.01730408","article-title":"Effects of rituximab on morphofunctional abnormalities of membranous glomerulopathy.","volume":"3","author":"Ruggenenti","year":"2008","journal-title":"Clin J Am Soc Nephrol"}],"container-title":["Journal of the American Society of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/00001751-201605000-00019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T19:27:14Z","timestamp":1786130834000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00001751-201605000-00019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,29]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.1681\/asn.2015030316","relation":{},"ISSN":["1046-6673","1533-3450"],"issn-type":[{"value":"1046-6673","type":"print"},{"value":"1533-3450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,29]]}}}