{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:34:58Z","timestamp":1789810498191,"version":"4.0.1"},"reference-count":51,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p>Studies have revealed many analogies between podocytes and neurons, and these analogies may be key to elucidating the pathogenesis of podocyte injury. Cathepsin D (CD) is a representative aspartic proteinase in lysosomes. Central nervous system neurons in CD-deficient mice exhibit a form of lysosomal storage disease with a phenotype resembling neuronal ceroid lipofuscinoses. In the kidney, the role of CD in podocytes has not been fully explored. Herein, we generated podocyte\u2013specific CD\u2013knockout mice that developed proteinuria at 5 months of age and ESRD by 20\u201322 months of age. Immunohistochemical analysis of these mice showed apoptotic podocyte death followed by proteinuria and glomerulosclerosis with aging. Using electron microscopy, we identified, in podocytes, granular osmiophilic deposits (GRODs), autophagosome\/autolysosome-like bodies, and fingerprint profiles, typical hallmarks of CD-deficient neurons. CD deficiency in podocytes also led to the cessation of autolysosomal degradation and accumulation of proteins indicative of autophagy impairment and the mitochondrial ATP synthase subunit c accumulation in the GRODs, again similar to changes reported in CD-deficient neurons. Furthermore, both podocin and nephrin, two essential components of the slit diaphragm, translocated to Rab7\u2013 and lysosome\u2013associated membrane glycoprotein 1\u2013positive amphisomes\/autolysosomes that accumulated in podocyte cell bodies in podocyte\u2013specific CD\u2013knockout mice. We hypothesize that defective lysosomal activity resulting in foot process effacement caused this accumulation of podocin and nephrin. Overall, our results suggest that loss of CD in podocytes causes autophagy impairment, triggering the accumulation of toxic subunit c\u2013positive lipofuscins as well as slit diaphragm proteins followed by apoptotic cell death.<\/jats:p>","DOI":"10.1681\/asn.2015040366","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T23:14:13Z","timestamp":1454022853000},"page":"2685-2700","source":"Crossref","is-referenced-by-count":54,"title":["Cathepsin D in Podocytes Is Important in the Pathogenesis of Proteinuria and CKD"],"prefix":"10.1681","volume":"27","author":[{"given":"Kanae","family":"Yamamoto-Nonaka","sequence":"first","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, Juntendo University Faculty of Medicine, Tokyo, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Koike","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and 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