{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T21:33:05Z","timestamp":1791495185749,"version":"4.3.4"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:p>\n                    Extracellular vesicles (ECVs) facilitate intercellular communication along the nephron, with the potential to change the function of the recipient cell. However, it is not known whether this is a regulated process analogous to other signaling systems. We investigated the potential hormonal regulation of ECV transfer and report that desmopressin, a vasopressin analogue, stimulated the uptake of fluorescently loaded ECVs into a kidney collecting duct cell line (mCCD\n                    <jats:sub>C11<\/jats:sub>\n                    ) and into primary cells. Exposure of mCCD\n                    <jats:sub>C11<\/jats:sub>\n                    cells to ECVs isolated from cells overexpressing microRNA-503 led to downregulated expression of microRNA-503 target genes, but only in the presence of desmopressin. Mechanistically, ECV entry into mCCD\n                    <jats:sub>C11<\/jats:sub>\n                    cells required cAMP production, was reduced by inhibiting dynamin, and was selective for ECVs from kidney tubular cells.\n                    <jats:italic toggle=\"yes\">In vivo<\/jats:italic>\n                    , we measured the urinary excretion and tissue uptake of fluorescently loaded ECVs delivered systemically to mice before and after administration of the vasopressin V2 receptor antagonist tolvaptan. In control-treated mice, we recovered 2.5% of administered ECVs in the urine; tolvaptan increased recovery five-fold and reduced ECV deposition in kidney tissue. Furthermore, in a patient with central diabetes insipidus, desmopressin reduced the excretion of ECVs derived from glomerular and proximal tubular cells. These data are consistent with vasopressin-regulated uptake of ECVs\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . We conclude that ECV uptake is a specific and regulated process. Physiologically, ECVs are a new mechanism of intercellular communication; therapeutically, ECVs may be a vehicle by which RNA therapy could be targeted to specific cells for the treatment of kidney disease.\n                  <\/jats:p>","DOI":"10.1681\/asn.2015050568","type":"journal-article","created":{"date-parts":[[2016,3,28]],"date-time":"2016-03-28T21:53:22Z","timestamp":1459202002000},"page":"3345-3355","source":"Crossref","is-referenced-by-count":59,"title":["Vasopressin Regulates Extracellular Vesicle Uptake by Kidney Collecting Duct Cells"],"prefix":"10.1681","volume":"27","author":[{"given":"Wilna","family":"Oosthuyzen","sequence":"first","affiliation":[{"name":"British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen M.","family":"Scullion","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica R.","family":"Ivy","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma E.","family":"Morrison","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Hunter","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J.","family":"Starkey Lewis","sequence":"additional","affiliation":[{"name":"Medical Research Council Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine, Edinburgh, United Kingdom; 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