{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:05:49Z","timestamp":1786133149863,"version":"build-2736575974"},"reference-count":49,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:p>\n                    Regulated intracellular proteostasis, controlled in part by proteolysis, is essential in maintaining the integrity of podocytes and the glomerular filtration barrier of the kidney. We applied a novel proteomics technology that enables proteome-wide identification, mapping, and quantification of protein N-termini to comprehensively characterize cleaved podocyte proteins in the glomerulus\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . We found evidence that defined proteolytic cleavage results in various proteoforms of important podocyte proteins, including those of podocin, nephrin, neph1,\n                    <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                    -actinin-4, and vimentin. Quantitative mapping of N-termini demonstrated perturbation of protease action during podocyte injury\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    , including diminished proteolysis of\n                    <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                    -actinin-4. Differentially regulated protease substrates comprised cytoskeletal proteins as well as intermediate filaments. Determination of preferential protease motifs during podocyte damage indicated activation of caspase proteases and inhibition of arginine-specific proteases. Several proteolytic processes were clearly site-specific, were conserved across species, and could be confirmed by differential migration behavior of protein fragments in gel electrophoresis. Some of the proteolytic changes discovered\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    also occurred in two\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    models of podocyte damage (WT1 heterozygous knockout mice and puromycin aminonucleoside\u2013treated rats). Thus, we provide direct and systems-level evidence that the slit diaphragm and podocyte cytoskeleton are regulated targets of proteolytic modification, which is altered upon podocyte damage.\n                  <\/jats:p>","DOI":"10.1681\/asn.2016101119","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T21:10:39Z","timestamp":1500498639000},"page":"2867-2878","source":"Crossref","is-referenced-by-count":42,"title":["N-Degradomic Analysis Reveals a Proteolytic Network Processing the Podocyte Cytoskeleton"],"prefix":"10.1681","volume":"28","author":[{"given":"Markus M.","family":"Rinschen","sequence":"first","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"},{"name":"Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), and"},{"name":"Systems Biology of Ageing Cologne (Sybacol), University of Cologne, Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Kathrin","family":"Hoppe","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Grahammer","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;"},{"name":"Department of Medicine IV, Medical Center and Faculty of Medicine \u2013 University of Freiburg, Freiburg, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kann","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linus A.","family":"V\u00f6lker","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva-Maria","family":"Schurek","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Binz","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"H\u00f6hne","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"},{"name":"Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), and"},{"name":"Systems Biology of Ageing Cologne (Sybacol), University of Cologne, Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih","family":"Demir","sequence":"additional","affiliation":[{"name":"Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum J\u00fclich, J\u00fclich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Malisic","sequence":"additional","affiliation":[{"name":"Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum J\u00fclich, J\u00fclich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias B.","family":"Huber","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;"},{"name":"Department of Medicine IV, Medical Center and Faculty of Medicine \u2013 University of Freiburg, Freiburg, Germany;"},{"name":"BIOSS Centre for Biological Signalling Studies and Center for Biological Systems Analysis (ZBSA), Albert-Ludwigs-University, Freiburg, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Kurschat","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayachandran N.","family":"Kizhakkedathu","sequence":"additional","affiliation":[{"name":"Centre for Blood Research, Department of Pathology and Laboratory Medicine, Department of Chemistry, University of British Columbia, Vancouver, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Schermer","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"},{"name":"Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), and"},{"name":"Systems Biology of Ageing Cologne (Sybacol), University of Cologne, Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pitter F.","family":"Huesgen","sequence":"additional","affiliation":[{"name":"Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungszentrum J\u00fclich, J\u00fclich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Benzing","sequence":"additional","affiliation":[{"name":"Department II of Internal Medicine,"},{"name":"Center for Molecular Medicine Cologne (CMMC),"},{"name":"Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), and"},{"name":"Systems Biology of Ageing Cologne (Sybacol), University of Cologne, Cologne, 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