{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:17:20Z","timestamp":1790029040727,"version":"4.0.1"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2018,9]]},"abstract":"\n Background<\/jats:title>\n \n The slit diaphragm is a specialized adhesion junction between opposing podocytes, establishing the final filtration barrier that prevents passage of proteins from the capillary lumen into the urinary space. Nephrin, the key structural and signaling adhesion molecule expressed in the slit diaphragm, contains an evolutionally conserved, atypical PDZ-binding motif (PBM) reported to bind to a variety of proteins in the slit diaphragm. Several mutations in\n NPHS1<\/jats:italic>\n (the gene encoding nephrin) that result in nephrin lacking an intact PBM are associated with glomerular diseases. However, the molecular basis of nephrin-PBM\u2013mediated protein complexes is still unclear.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Using a combination of biochemic, biophysic, and cell biologic approaches, we systematically investigated the interactions between nephrin-PBM and PDZ domain\u2013containing proteins in the slit diaphragm.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n We found that nephrin-PBM specifically binds to one member of the membrane-associated guanylate kinase family of scaffolding proteins, MAGI1, but not to another, MAGI2. The complex structure of MAGI1-PDZ3\/nephrin-PBM reveals that the Gly at the \u22123 position of nephrin-PBM is the determining feature for MAGI1-PDZ3 recognition, which sharply contrasts with the typical PDZ\/PBM binding mode. A single gain-of-function mutation within MAGI2 enabled nephrin-PBM binding. In addition, using our structural analysis, we developed a highly efficient inhibitory peptide capable of specifically blocking the nephrin\/MAGI1 interaction.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n MAGI1 interacts with nephrin-PBM with exquisite specificity. A newly developed, potent inhibitory peptide that blocks this interaction may be useful for future functional investigations\n in vivo<\/jats:italic>\n . Our findings also provide possible explanations for the diseases caused by\n NPHS1<\/jats:italic>\n mutations.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1681\/asn.2017121275","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T15:35:37Z","timestamp":1531496137000},"page":"2362-2371","source":"Crossref","is-referenced-by-count":12,"title":["Structural Basis of Highly Specific Interaction between Nephrin and MAGI1 in Slit Diaphragm Assembly and Signaling"],"prefix":"10.1681","volume":"29","author":[{"given":"Zhuangfeng","family":"Weng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Molecular Biology, Shanghai Science Research Center, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China;"},{"name":"School of Life Science and Technology, ShanghaiTech University, Shanghai, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Shang","sequence":"additional","affiliation":[{"name":"Division of Life Science, State Key Laboratory of Molecular Neuroscience, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyang","family":"Ji","sequence":"additional","affiliation":[{"name":"Division of Life Science, State Key Laboratory of Molecular Neuroscience, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Ye","sequence":"additional","affiliation":[{"name":"Division of Life Science, State Key Laboratory of Molecular Neuroscience, and"},{"name":"Center of Systems Biology and Human Health, Hong Kong University of Science and Technology, Kowloon, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Biology, Shanghai Science Research Center, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongguang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Biology, Shanghai Science Research Center, Chinese Academy of Sciences Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China;"},{"name":"School of Life Science and Technology, ShanghaiTech University, Shanghai, China; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwei","family":"Zhu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Molecular Biology, Shanghai Science Research Center, Chinese Academy of Sciences Center for 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