{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:57:07Z","timestamp":1787763427164,"version":"build-2784847793"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JASN"],"published-print":{"date-parts":[[2019,2]]},"abstract":"\n Significance Statement<\/jats:title>\n \n No effective therapy is currently available to directly address a key feature of CKD progression: interstitial fibrosis leading to a loss of peritubular capillaries (PTCs). One potential antifibrotic candidate, hepatocyte growth factor (HGF), cannot be applied in clinical settings owing to its short\n t<\/jats:italic>\n 1\/2<\/jats:sub>\n in blood and elimination by other organs, such as the liver. The authors describe use of a novel HGF therapy using cell sheet technology: transplantation of transgenic HGF-secreting mesothelial cell sheets to the kidney in a CKD rat model. The HGF-secreting cell sheet directly suppresses interstitial fibrosis for 28 days and prevents loss of peritubular capillaries. Inhibition of CKD progression requires long-term suppression of fibrosis, and cell sheet therapy represents a promising strategy for achieving that goal.\n <\/jats:p>\n <\/jats:sec>\n \n Background<\/jats:title>\n Although hepatocyte growth factor (HGF) has antifibrotic effects and is involved in angiogenesis and vasodilation, systemic administration of HGF to prevent kidney fibrosis is not a feasible strategy for suppressing interstitial fibrosis in patients with CKD.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We investigated a novel therapy involving HGF transgenic cell sheets grown in culture from human mesothelial cells and administered to rats with unilateral ureteral obstruction (UUO). We compared progression of fibrosis in rats with UUO that received one of five interventions: HGF-transgenic mesothelial cell sheets transplanted to the kidney surface, HGF-transgenic mesothelial cell sheets transplanted to thigh, mesotherial cell sheets transplanted to kidney, no sheets, or HGF injections.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n HGF transgenic cell sheets transplanted to the kidney strongly suppressed the induction of myofibroblasts and collagen in the kidney for 28 days; other interventions did not. Additionally, the HGF-secreting cell sheets ameliorated loss of peritubular capillaries and maintained renal blood flow.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n These findings suggest that cell sheet therapy is a novel and promising strategy for inhibiting progressive fibrosis in CKD.<\/jats:p>\n <\/jats:sec>","DOI":"10.1681\/asn.2018050556","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T17:35:16Z","timestamp":1547228116000},"page":"261-276","source":"Crossref","is-referenced-by-count":33,"title":["Hepatocyte Growth Factor\u2013Secreting Mesothelial Cell Sheets Suppress Progressive Fibrosis in a Rat Model of CKD"],"prefix":"10.1681","volume":"30","author":[{"given":"Masatoshi","family":"Oka","sequence":"first","affiliation":[{"name":"Department of Medicine, Kidney Center,"},{"name":"Institute of Advanced Biomedical Engineering and Science, 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