{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:37:02Z","timestamp":1787312222477,"version":"build-2736575974"},"reference-count":64,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","funder":[{"name":"Novo Nordisk Young Investigator Award","award":["NNF19OC0056043"],"award-info":[{"award-number":["NNF19OC0056043"]}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["JASN"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec>\n                    <jats:title>Significance Statement<\/jats:title>\n                    <jats:p>\n                      Renal blood filtration occurs in a functional unit called the glomerulus. The filtration barrier comprises resident cell types,\n                      <jats:italic toggle=\"yes\">i.e.<\/jats:italic>\n                      , podocytes, mesangial cells, and glomerular endothelial cells. We introduce a glomerular cell isolation protocol that separates these three cell types at a sufficient quantity and purity to allow for detailed protein-biochemical investigations. We demonstrate that the expression of fluorescent transgenes in glomerular cells can result in proteome artifacts, and that different mouse strains have different glomerular cell type proteomes. Further, we demonstrate the power of this globally applicable technique to identify new proteins enriched in glomerular cells and to dissect cell-specific disease responses and crosstalk between different intraglomerular cell types.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The glomerulus comprises podocytes, mesangial cells, and endothelial cells, which jointly determine glomerular filtration. Understanding this intricate functional unit beyond the transcriptome requires bulk isolation of these cell types for biochemical investigations. We developed a globally applicable tripartite isolation method for murine mesangial and endothelial cells and podocytes (timMEP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We separated glomerular cell types from wild-type or mT\/mG mice\n                      <jats:italic toggle=\"yes\">via<\/jats:italic>\n                      a novel FACS approach, and validated their purity. Cell type proteomes were compared between strains, ages, and sex. We applied timMEP to the podocyte-targeting, immunologic, THSD7A-associated, model of membranous nephropathy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>timMEP enabled protein-biochemical analyses of podocytes, mesangial cells, and endothelial cells derived from reporter-free mice, and allowed for the characterization of podocyte, endothelial, and mesangial proteomes of individual mice. We identified marker proteins for mesangial and endothelial proteins, and outlined protein-based, potential communication networks and phosphorylation patterns. The analysis detected cell type\u2013specific proteome differences between mouse strains and alterations depending on sex, age, and transgene. After exposure to anti-THSD7A antibodies, timMEP resolved a fine-tuned initial stress response, chiefly in podocytes, that could not be detected by bulk glomerular analyses. The combination of proteomics with super-resolution imaging revealed a specific loss of slit diaphragm, but not of other foot process proteins, unraveling a protein-based mechanism of podocyte injury in this animal model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>timMEP enables glomerular cell type\u2013resolved investigations at the transcriptional and protein-biochemical level in health and disease, while avoiding reporter-based artifacts, paving the way toward the comprehensive and systematic characterization of glomerular cell biology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1681\/asn.2020091346","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T14:46:09Z","timestamp":1622558769000},"page":"2175-2193","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":26,"title":["Tripartite Separation of Glomerular Cell Types and Proteomes from Reporter-Free Mice"],"prefix":"10.1681","volume":"32","author":[{"given":"Favian A.","family":"Hatje","sequence":"first","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uta","family":"Wedekind","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebke","family":"Sachs","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0827-149X","authenticated-orcid":false,"given":"Desiree","family":"Loreth","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Reichelt","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5744-0205","authenticated-orcid":false,"given":"Fatih","family":"Demir","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Aarhus University, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7425-4535","authenticated-orcid":false,"given":"Christopher","family":"Kosub","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1889-0470","authenticated-orcid":false,"given":"Lukas","family":"Heintz","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola M.","family":"Tomas","sequence":"additional","affiliation":[{"name":"III Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias B.","family":"Huber","sequence":"additional","affiliation":[{"name":"III Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinah","family":"Skuza","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlies","family":"Sachs","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Zielinski","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus M.","family":"Rinschen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine, Aarhus University, Aarhus, Denmark"},{"name":"III Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"},{"name":"Department II of Internal Medicine, Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Meyer-Schwesinger","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2021,9]]},"reference":[{"key":"B1-20240826","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1152\/physrev.00020.2002","article-title":"Cell biology of the glomerular podocyte","volume":"83","author":"Pavenst\u00e4dt","year":"2003","journal-title":"Physiol Rev"},{"key":"B2-20240826","first-page":"231","article-title":"Crosstalk in glomerular injury and repair","volume":"24","author":"Dimke","year":"2015","journal-title":"Curr Opin Nephrol Hypertens"},{"key":"B3-20240826","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/S0002-9440(10)64239-3","article-title":"A new method for large scale isolation of kidney glomeruli from mice","volume":"161","author":"Takemoto","year":"2002","journal-title":"Am J Pathol"},{"key":"B4-20240826","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1681\/ASN.2013050452","article-title":"Unraveling the role of podocyte turnover in glomerular aging and injury","volume":"25","author":"Wanner","year":"2014","journal-title":"J Am Soc Nephrol"},{"key":"B5-20240826","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1038\/ki.2012.487","article-title":"Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks","volume":"83","author":"Boerries","year":"2013","journal-title":"Kidney Int"},{"key":"B6-20240826","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1681\/ASN.2019030312","article-title":"Proteome analysis of isolated podocytes reveals stress responses in glomerular sclerosis","volume":"31","author":"Koehler","year":"2020","journal-title":"J Am Soc Nephrol"},{"key":"B7-20240826","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1002\/dvg.20335","article-title":"A global double-fluorescent Cre reporter mouse","volume":"45","author":"Muzumdar","year":"2007","journal-title":"Genesis"},{"key":"B8-20240826","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.kint.2016.12.011","article-title":"Construction of a viral T2A-peptide based knock-in mouse model for enhanced Cre recombinase activity and fluorescent labeling of podocytes","volume":"91","author":"Koehler","year":"2017","journal-title":"Kidney Int"},{"key":"B9-20240826","doi-asserted-by":"crossref","first-page":"3262","DOI":"10.1681\/ASN.2017010030","article-title":"A heterologous model of thrombospondin type 1 domain-containing 7A-associated membranous nephropathy","volume":"28","author":"Tomas","year":"2017","journal-title":"J Am Soc Nephrol"},{"key":"B10-20240826","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1038\/sj.ki.5002450","article-title":"A new mouse model of immune-mediated podocyte injury","volume":"72","author":"Meyer","year":"2007","journal-title":"Kidney Int"},{"key":"B11-20240826","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1016\/j.kint.2017.12.012","article-title":"Single-nephron proteomes connect morphology and function in proteinuric kidney disease","volume":"93","author":"H\u00f6hne","year":"2018","journal-title":"Kidney Int"},{"key":"B12-20240826","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.mcb.2019.03.010","article-title":"Single glomerular proteomics: A novel tool for translational glomerular cell biology","volume":"154","author":"Rinschen","year":"2019","journal-title":"Methods Cell Biol"},{"key":"B13-20240826","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s41596-018-0082-x","article-title":"Single-pot, solid-phase-enhanced sample preparation for proteomics experiments","volume":"14","author":"Hughes","year":"2019","journal-title":"Nat Protoc"},{"key":"B14-20240826","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1016\/j.celrep.2018.04.059","article-title":"A multi-layered quantitative in vivo expression atlas of the podocyte unravels kidney disease candidate genes","volume":"23","author":"Rinschen","year":"2018","journal-title":"Cell Rep"},{"key":"B15-20240826","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat Biotechnol"},{"key":"B16-20240826","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1074\/mcp.M113.031591","article-title":"Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ","volume":"13","author":"Cox","year":"2014","journal-title":"Mol Cell Proteomics"},{"key":"B17-20240826","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote)omics data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nat Methods"},{"key":"B18-20240826","doi-asserted-by":"crossref","first-page":"D362","DOI":"10.1093\/nar\/gkw937","article-title":"The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible","volume":"45","author":"Szklarczyk","year":"2017","journal-title":"Nucleic Acids Res"},{"key":"B19-20240826","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res"},{"key":"B20-20240826","doi-asserted-by":"crossref","first-page":"eaax9760","DOI":"10.1126\/scisignal.aax9760","article-title":"Metabolic rewiring of the hypertensive kidney","volume":"12","author":"Rinschen","year":"2019","journal-title":"Sci Signal"},{"key":"B21-20240826","doi-asserted-by":"crossref","first-page":"W281","DOI":"10.1093\/nar\/gks469","article-title":"Seq2Logo: A method for construction and visualization of amino acid binding motifs and sequence profiles including sequence weighting, pseudo counts and two-sided representation of amino acid enrichment and depletion","volume":"40","author":"Thomsen","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"B22-20240826","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1681\/ASN.2005040364","article-title":"Compartment-specific expression and function of the chemokine IP-10\/CXCL10 in a model of renal endothelial microvascular injury","volume":"17","author":"Panzer","year":"2006","journal-title":"J Am Soc Nephrol"},{"key":"B23-20240826","first-page":"36","article-title":"Image Processing with ImageJ","volume":"11","author":"Abramoff","year":"2004","journal-title":"Biophotonics Int"},{"key":"B24-20240826","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/1478-811X-11-76","article-title":"The proteases HtrA2\/Omi and UCH-L1 regulate TNF-induced necroptosis","volume":"11","author":"Sosna","year":"2013","journal-title":"Cell Commun Signal"},{"key":"B25-20240826","doi-asserted-by":"crossref","first-page":"292","DOI":"10.3389\/fmed.2018.00292","article-title":"Comparative Analysis of podocyte foot process morphology in three species by 3D super-resolution microscopy","volume":"5","author":"Artelt","year":"2018","journal-title":"Front Med (Lausanne)"},{"key":"B26-20240826","doi-asserted-by":"crossref","first-page":"11473","DOI":"10.1038\/s41598-017-11553-x","article-title":"Structured illumination microscopy and automatized image processing as a rapid diagnostic tool for podocyte effacement","volume":"7","author":"Siegerist","year":"2017","journal-title":"Sci Rep"},{"key":"B27-20240826","doi-asserted-by":"crossref","first-page":"e34907","DOI":"10.1371\/journal.pone.0034907","article-title":"Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin","volume":"7","author":"Kabgani","year":"2012","journal-title":"PLoS One"},{"key":"B28-20240826","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1097\/MOH.0000000000000239","article-title":"Endothelial functions of platelet\/endothelial cell adhesion molecule-1 (CD31)","volume":"23","author":"Lertkiatmongkol","year":"2016","journal-title":"Curr Opin Hematol"},{"key":"B29-20240826","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1172\/JCI21851","article-title":"Impairment of tubuloglomerular feedback regulation of GFR in ecto-5\u2032-nucleotidase\/CD73-deficient mice","volume":"114","author":"Castrop","year":"2004","journal-title":"J Clin Invest"},{"key":"B30-20240826","doi-asserted-by":"crossref","first-page":"2060","DOI":"10.1681\/ASN.2018030238","article-title":"A single-cell transcriptome atlas of the mouse glomerulus","volume":"29","author":"Karaiskos","year":"2018","journal-title":"J Am Soc Nephrol"},{"key":"B31-20240826","doi-asserted-by":"crossref","first-page":"226","DOI":"10.3389\/fendo.2014.00226","article-title":"Genes and podocytes - new insights into mechanisms of podocytopathy","volume":"5","author":"Bierzynska","year":"2015","journal-title":"Front Endocrinol (Lausanne)"},{"key":"B32-20240826","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1681\/ASN.2013070760","article-title":"Phosphoproteomic analysis reveals regulatory mechanisms at the kidney filtration barrier","volume":"25","author":"Rinschen","year":"2014","journal-title":"J Am Soc Nephrol"},{"key":"B33-20240826","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.1002\/pmic.201400235","article-title":"Comparative phosphoproteomic analysis of mammalian glomeruli reveals conserved podocin C-terminal phosphorylation as a determinant of slit diaphragm complex architecture","volume":"15","author":"Rinschen","year":"2015","journal-title":"Proteomics"},{"key":"B34-20240826","doi-asserted-by":"crossref","first-page":"e8918","DOI":"10.1371\/journal.pone.0008918","article-title":"Automated network analysis identifies core pathways in glioblastoma","volume":"5","author":"Cerami","year":"2010","journal-title":"PLoS One"},{"key":"B35-20240826","doi-asserted-by":"crossref","first-page":"36140","DOI":"10.1074\/jbc.M114.557124","article-title":"The ubiquitin C-terminal hydrolase L1 (UCH-L1) C terminus plays a key role in protein stability, but its farnesylation is not required for membrane association in primary neurons","volume":"289","author":"Bishop","year":"2014","journal-title":"J Biol Chem"},{"key":"B36-20240826","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.4049\/jimmunol.171.3.1255","article-title":"A giant GTPase, very large inducible GTPase-1, is inducible by IFNs","volume":"171","author":"Klamp","year":"2003","journal-title":"J Immunol"},{"key":"B37-20240826","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1681\/ASN.2018090941","article-title":"Thrombospondin type 1 domain-containing 7A localizes to the slit diaphragm and stabilizes membrane dynamics of fully differentiated podocytes","volume":"30","author":"Herwig","year":"2019","journal-title":"J Am Soc Nephrol"},{"key":"B38-20240826","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1056\/NEJMoa1409354","article-title":"Thrombospondin type-1 domain-containing 7A in idiopathic membranous nephropathy","volume":"371","author":"Tomas","year":"2014","journal-title":"N Engl J Med"},{"key":"B39-20240826","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1177\/002215549804600104","article-title":"Agrin is a major heparan sulfate proteoglycan in the human glomerular basement membrane","volume":"46","author":"Groffen","year":"1998","journal-title":"J Histochem Cytochem"},{"key":"B40-20240826","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1046\/j.1523-1755.2003.00800.x","article-title":"Laminin-1 reexpression in Alport mouse glomerular basement membranes","volume":"63","author":"Abrahamson","year":"2003","journal-title":"Kidney Int"},{"key":"B41-20240826","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1159\/000101798","article-title":"Role of the VEGF--a signaling pathway in the glomerulus: evidence for crosstalk between components of the glomerular filtration barrier","volume":"106","author":"Eremina","year":"2007","journal-title":"Nephron, Physiol"},{"key":"B42-20240826","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1074\/jbc.M114.588798","article-title":"Insights into the role of the unusual disulfide bond in copper-zinc superoxide dismutase","volume":"290","author":"Sea","year":"2015","journal-title":"J Biol Chem"},{"key":"B43-20240826","doi-asserted-by":"crossref","first-page":"2031","DOI":"10.1002\/pro.505","article-title":"Human pyruvate kinase M2: A multifunctional protein","volume":"19","author":"Gupta","year":"2010","journal-title":"Protein Sci"},{"key":"B44-20240826","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.bbalip.2009.05.006","article-title":"Mitochondrial long chain fatty acid beta-oxidation in man and mouse","volume":"1791","author":"Chegary","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"B45-20240826","doi-asserted-by":"crossref","first-page":"e86177","DOI":"10.1172\/jci.insight.86177","article-title":"A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes","volume":"1","author":"Grahammer","year":"2016","journal-title":"JCI Insight"},{"key":"B46-20240826","article-title":"Experimental and computational technologies to dissect the kidney at the single-cell level","author":"Kuppe","journal-title":"Nephrol Dial Transplant"},{"key":"B47-20240826","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1002\/path.2446","article-title":"A new role for the neuronal ubiquitin C-terminal hydrolase-L1 (UCH-L1) in podocyte process formation and podocyte injury in human glomerulopathies","volume":"217","author":"Meyer-Schwesinger","year":"2009","journal-title":"J Pathol"},{"key":"B48-20240826","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1038\/ki.2013.115","article-title":"The expression of podocyte-specific proteins in parietal epithelial cells is regulated by protein degradation","volume":"84","author":"Guhr","year":"2013","journal-title":"Kidney Int"},{"key":"B49-20240826","doi-asserted-by":"crossref","first-page":"F1812","DOI":"10.1152\/ajprenal.00380.2018","article-title":"The podocyte protease web: Uncovering the gatekeepers of glomerular disease","volume":"315","author":"Rinschen","year":"2018","journal-title":"Am J Physiol Renal Physiol"},{"key":"B50-20240826","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.kint.2018.11.028","article-title":"Representation and relative abundance of cell-type selective markers in whole-kidney RNA-Seq data","volume":"95","author":"Clark","year":"2019","journal-title":"Kidney Int"},{"key":"B51-20240826","doi-asserted-by":"crossref","first-page":"3218","DOI":"10.4049\/jimmunol.1003451","article-title":"Nephrotic syndrome and subepithelial deposits in a mouse model of immune-mediated anti-podocyte glomerulonephritis","volume":"187","author":"Meyer-Schwesinger","year":"2011","journal-title":"J Immunol"},{"key":"B52-20240826","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1681\/ASN.2014040419","article-title":"Genetic background is a key determinant of glomerular extracellular matrix composition and organization","volume":"26","author":"Randles","year":"2015","journal-title":"J Am Soc Nephrol"},{"key":"B53-20240826","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1021\/acs.molpharmaceut.0c00094","article-title":"Cyclo-RGD truncated polymeric nanoconstruct with dendrimeric templates for targeted HDAC4 gene silencing in a diabetic nephropathy mouse model","volume":"18","author":"Raval","year":"2021","journal-title":"Mol Pharm"},{"key":"B54-20240826","doi-asserted-by":"crossref","first-page":"4091","DOI":"10.1172\/JCI81061","article-title":"MicroRNA-30 family members regulate calcium\/calcineurin signaling in podocytes","volume":"125","author":"Wu","year":"2015","journal-title":"J Clin Invest"},{"key":"B55-20240826","doi-asserted-by":"crossref","first-page":"C404","DOI":"10.1152\/ajpcell.00121.2016","article-title":"Quantitative deep mapping of the cultured podocyte proteome uncovers shifts in proteostatic mechanisms during differentiation","volume":"311","author":"Rinschen","year":"2016","journal-title":"Am J Physiol Cell Physiol"},{"key":"B56-20240826","doi-asserted-by":"crossref","first-page":"4696","DOI":"10.1096\/fj.201701307R","article-title":"Protein half-life determines expression of proteostatic networks in podocyte differentiation","volume":"32","author":"Schroeter","year":"2018","journal-title":"FASEB J"},{"key":"B57-20240826","doi-asserted-by":"crossref","first-page":"14450","DOI":"10.1096\/fj.201900978RR","article-title":"Fibronectin is up-regulated in podocytes by mechanical stress","volume":"33","author":"Kliewe","year":"2019","journal-title":"FASEB J"},{"key":"B58-20240826","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1038\/nm.2411","article-title":"Circulating urokinase receptor as a cause of focal segmental glomerulosclerosis","volume":"17","author":"Wei","year":"2011","journal-title":"Nat Med"},{"key":"B59-20240826","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nm1696","article-title":"Modification of kidney barrier function by the urokinase receptor","volume":"14","author":"Wei","year":"2008","journal-title":"Nat Med"},{"key":"B60-20240826","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1681\/ASN.2018090912","article-title":"Advantages of single-nucleus over single-cell RNA sequencing of adult kidney: Rare cell types and novel cell states revealed in fibrosis","volume":"30","author":"Wu","year":"2019","journal-title":"J Am Soc Nephrol"},{"key":"B61-20240826","doi-asserted-by":"crossref","first-page":"758","DOI":"10.1126\/science.aar2131","article-title":"Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease","volume":"360","author":"Park","year":"2018","journal-title":"Science"},{"key":"B62-20240826","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s41581-020-00348-5","article-title":"The tissue proteome in the multi-omic landscape of kidney disease","volume":"17","author":"Rinschen","year":"2021","journal-title":"Nat Rev Nephrol"},{"key":"B63-20240826","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE database and related tools and resources in 2019: Improving support for quantification data","volume":"47","author":"Perez-Riverol","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"B64-20240826","doi-asserted-by":"crossref","first-page":"D447","DOI":"10.1093\/nar\/gkv1145","article-title":"2016 update of the PRIDE database and its related tools [published correction appears in Nucleic Acid Res 44: 11033, 2016 10.1093\/nar\/gkw880]","volume":"44","author":"Vizca\u00edno","year":"2016","journal-title":"Nucleic Acids Res"}],"container-title":["Journal of the American Society of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.1681\/ASN.2020091346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T19:25:03Z","timestamp":1786130703000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.1681\/ASN.2020091346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9]]},"references-count":64,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.1681\/asn.2020091346","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.08.22.262584","asserted-by":"object"}]},"ISSN":["1046-6673","1533-3450"],"issn-type":[{"value":"1046-6673","type":"print"},{"value":"1533-3450","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9]]},"assertion":[{"value":"2020-09-20","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-09","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}