{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:04:29Z","timestamp":1786133069101,"version":"build-2736575974"},"reference-count":43,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","funder":[{"DOI":"10.13039\/100006545","name":"National Institute of Minority Health and Health Disparities","doi-asserted-by":"publisher","award":["U54 MD0007586"],"award-info":[{"award-number":["U54 MD0007586"]}],"id":[{"id":"10.13039\/100006545","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2016YFC1305400"],"award-info":[{"award-number":["2016YFC1305400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870482"],"award-info":[{"award-number":["81870482"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870486"],"award-info":[{"award-number":["81870486"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2019-I2M-5-046"],"award-info":[{"award-number":["2019-I2M-5-046"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"US Department of Defense","doi-asserted-by":"publisher","award":["LR190150"],"award-info":[{"award-number":["LR190150"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015502","name":"Dialysis Clinic","doi-asserted-by":"publisher","award":["2018-04"],"award-info":[{"award-number":["2018-04"]}],"id":[{"id":"10.13039\/100015502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":["JASN"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:sec>\n                    <jats:title>Significance Statement<\/jats:title>\n                    <jats:p>Antiglomerular basement membrane (GBM) disease clinically presents as rapidly progressive GN, often accompanied by pulmonary hemorrhage. The autoantibodies that mediate the disease typically recognize cryptic epitopes within \u03b1345(IV) collagen in the glomerular and alveolar basement membranes. Laminin-521 is another major autoantigen that autoantibodies in anti-GBM disease target. These autoantibodies were detected in 51.5% of patients with both kidney and lung involvement of anti-GBM disease, compared with 23.5% of patients with isolated kidney disease. Seropositivity for antilaminin autoantibodies was significantly associated with lung hemorrhage, hemoptysis, and smoking. Antilaminin autoantibodies may contribute to lung injury in anti-GBM disease by increasing the amount of IgG bound to the alveolar basement membrane.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Antiglomerular basement membrane (anti-GBM) disease is characterized by GN and often pulmonary hemorrhage, mediated by autoantibodies that typically recognize cryptic epitopes within \u03b1345(IV) collagen\u2014a major component of the glomerular and alveolar basement membranes. Laminin-521 is another major GBM component and a proven target of pathogenic antibodies mediating GN in animal models. Whether laminin-521 is a target of autoimmunity in human anti-GBM disease is not yet known.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A retrospective study of circulating autoantibodies from 101 patients with anti-GBM\/Goodpasture\u2019s disease and 85 controls used a solid-phase immunoassay to measure IgG binding to human recombinant laminin-521 with native-like structure and activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Circulating IgG autoantibodies binding to laminin-521 were found in about one third of patients with anti-GBM antibody GN, but were not detected in healthy controls or in patients with other glomerular diseases. Autoreactivity toward laminin-521 was significantly more common in patients with anti-GBM GN and lung hemorrhage, compared with those with kidney-limited disease (51.5% versus 23.5%,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.005). Antilaminin-521 autoantibodies were predominantly of IgG1 and IgG4 subclasses and significantly associated with lung hemorrhage (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.005), hemoptysis (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.008), and smoking (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      =0.01), although not with proteinuria or serum creatinine at diagnosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Besides \u03b1345(IV) collagen, laminin-521 is another major autoantigen targeted in anti-GBM disease. Autoantibodies to laminin-521 may have the potential to promote lung injury in anti-GBM disease by increasing the total amount of IgG bound to the alveolar basement membranes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1681\/asn.2020101431","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T14:17:10Z","timestamp":1619187430000},"page":"1887-1897","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":26,"title":["Laminin-521 is a Novel Target of Autoantibodies Associated with Lung Hemorrhage in Anti-GBM Disease"],"prefix":"10.1681","volume":"32","author":[{"given":"Cong-rong","family":"Shen","sequence":"first","affiliation":[{"name":"Renal Division, Institute of Nephrology, Peking University First Hospital, Key Laboratory of Renal Disease, Ministry of Health of China, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-yu","family":"Jia","sequence":"additional","affiliation":[{"name":"Renal Division, Institute of Nephrology, Peking University First Hospital, Key Laboratory of Renal Disease, Ministry of Health of China, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentian","family":"Luo","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Vanderbilt University Medical Center, Nashville, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florina","family":"Olaru","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Vanderbilt University Medical Center, Nashville, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Cui","sequence":"additional","affiliation":[{"name":"Renal Division, Institute of Nephrology, Peking University First Hospital, Key Laboratory of Renal Disease, Ministry of Health of China, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3340-3108","authenticated-orcid":false,"given":"Ming-hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Renal Division, Institute of Nephrology, Peking University First Hospital, Key Laboratory of Renal Disease, Ministry of Health of China, Beijing, China"},{"name":"Peking-Tsinghua Center for Life Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4568-279X","authenticated-orcid":false,"given":"Dorin-Bogdan","family":"Borza","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Physiology, Meharry Medical College, Nashville, Tennessee"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2021,8]]},"reference":[{"key":"B1-20240826","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/nri3523","article-title":"The immune system and kidney disease: Basic concepts and clinical implications","volume":"13","author":"Kurts","year":"2013","journal-title":"Nat Rev Immunol"},{"key":"B2-20240826","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.jaut.2014.01.024","article-title":"Diagnosis and classification of Goodpasture\u2019s disease (anti-GBM)","volume":"48\u201349","author":"Hellmark","year":"2014","journal-title":"J Autoimmun"},{"key":"B3-20240826","doi-asserted-by":"crossref","first-page":"6030","DOI":"10.1074\/jbc.275.8.6030","article-title":"The goodpasture autoantigen. 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