{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:05:22Z","timestamp":1786133122845,"version":"build-2736575974"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract.<\/jats:title>\n                    <jats:p>\n                      Tubulointerstitial nephritis is the main manifestation of acute renal damage caused by leptospirosis, but the mechanism remains unexplored. Patients infected with\n                      <jats:italic toggle=\"yes\">Leptospira shermani<\/jats:italic>\n                      in Taiwan disclosed tubular dysfunction particularly in the medullary thick ascending limb of loop of Henle (mTAL), and the related renal damage seems to be underestimated. To elucidate the mechanism of tubular damage, outer membrane protein extract from\n                      <jats:italic toggle=\"yes\">Leptospira<\/jats:italic>\n                      was administered to a model of cultured mTAL cells derived from normal mice. The addition of outer membrane protein extract from\n                      <jats:italic toggle=\"yes\">L. shermani<\/jats:italic>\n                      to cultured mTAL cells induced a significant nuclear DNA binding of the NF-\u03ba B transcription factor by electrophoresis mobility shift assay. Forty-eight h after adding the outer membrane protein extract (0.2 \u03bcg\/ml) to the cultured cells, the expression of inducible nitric oxide mRNA increased by 4.2-fold, monocyte chemoattractant protein-1 by 3-fold, and tumor necrosis factor-\u03b1 by 2.4-fold when compared with untreated cells examined by reverse transcription competitive-PCR. Supernatant nitrite, monocyte chemoattractant protein-1, and tumor necrosis factor-\u03b1 protein levels also increased by 1.8-, 7.1-, and 5-fold, respectively. An antiserum raised against\n                      <jats:italic toggle=\"yes\">L. shermani<\/jats:italic>\n                      largely prevented these effects. Outer membrane protein extract from\n                      <jats:italic toggle=\"yes\">L. bratislava<\/jats:italic>\n                      induced fewer effects than\n                      <jats:italic toggle=\"yes\">L. shermani<\/jats:italic>\n                      , and the avirulent nonpathogenic\n                      <jats:italic toggle=\"yes\">L. biflexa<\/jats:italic>\n                      serovar\n                      <jats:italic toggle=\"yes\">patoc<\/jats:italic>\n                      did not induce significant effects in the mTAL cells. In conclusion,\n                      <jats:italic toggle=\"yes\">L. shermani<\/jats:italic>\n                      infection may cause mTAL cell damage and inflammation through the NF-\u03ba B\u2014associated pathway. Findings of this study may be important in understanding the pathogenesis of tubulointerstitial nephritis caused by these organisms.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1681\/asn.v11112017","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T07:33:18Z","timestamp":1619595198000},"page":"2017-2026","source":"Crossref","is-referenced-by-count":54,"title":["Leptospira Outer Membrane Protein Activates NF-\u03baB and Downstream Genes Expressed in Medullary Thick Ascending Limb Cells"],"prefix":"10.1681","volume":"11","author":[{"given":"CHIH-WEI","family":"YANG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MAI-SZU","family":"WU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MING-JENG","family":"PAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JENN-JYE","family":"HONG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CHUN-CHEN","family":"YU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALAIN","family":"VANDEWALLE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CHIU-CHING","family":"HUANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Journal of the American Society of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/00001751-200011000-00008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T19:29:01Z","timestamp":1786130941000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00001751-200011000-00008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2000]]}},"URL":"https:\/\/doi.org\/10.1681\/asn.v11112017","relation":{},"ISSN":["1046-6673"],"issn-type":[{"value":"1046-6673","type":"print"}],"subject":[],"published":{"date-parts":[[2000,11]]}}}