{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T20:10:40Z","timestamp":1786133440562,"version":"build-2736575974"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract.<\/jats:title>\n                    <jats:p>\n                      The two-electrode voltage clamp technique was used to demonstrate translocation of\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      -aminohippurate (PAH) and related compounds such as loop diuretics in\n                      <jats:italic toggle=\"yes\">Xenopus laevis<\/jats:italic>\n                      oocytes expressing the renal organic anion transporter from winter flounder kidney (fROAT). In fROAT-expressing oocytes, PAH (0.1 mM) induced a depolarization of 4.2 \u00b1 0.4 mV and at a holding potential of -60 mV an inward current of -22.6 \u00b1 3.5 nA. PAH-induced current and the current calculated from [\n                      <jats:sup>3<\/jats:sup>\n                      H]-PAH uptake were of similar magnitude. Depolarization, inward current, and current-to-uptake relation indicated exchange of the monovalent PAH with a divalent anion, possibly \u03b1-ketoglutarate (\u03b1-KG), causing net efflux of one negative charge. The kinetic analysis of PAH-induced currents revealed that translocation is dependent on membrane potential, saturable with an apparent\n                      <jats:italic toggle=\"yes\">K<\/jats:italic>\n                      <jats:sub>m<\/jats:sub>\n                      of 58 \u00b1 8 \u03bcM, and sensitive to probenecid and furosemide. In contrast to probenecid and furosemide, the loop diuretics bumetanide, ethacrynic acid, and tienilic acid and the nephrotoxic mycotoxin ochratoxin A elicited inward currents indicating translocation through fROAT. Substrate-dependent currents provide a tool to elucidate the structure\/function relationship of the renal organic anion transporter.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1681\/asn.v1119","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T20:52:40Z","timestamp":1620247960000},"page":"9-17","source":"Crossref","is-referenced-by-count":22,"title":["Electrophysiologic Characterization of an Organic Anion Transporter Cloned from Winter Flounder Kidney (fROAT)"],"prefix":"10.1681","volume":"11","author":[{"given":"BIRGITTA CHRISTINA","family":"BURCKHARDT","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NATASCHA A.","family":"WOLFF","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GERHARD","family":"BURCKHARDT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Journal of the American Society of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/00001751-200001000-00002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T19:34:00Z","timestamp":1786131240000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00001751-200001000-00002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000]]}},"URL":"https:\/\/doi.org\/10.1681\/asn.v1119","relation":{},"ISSN":["1046-6673"],"issn-type":[{"value":"1046-6673","type":"print"}],"subject":[],"published":{"date-parts":[[2000,1]]}}}