{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:48:05Z","timestamp":1790974085314,"version":"4.1.0"},"reference-count":0,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract.<\/jats:title>\n                    <jats:p>\n                      Mutations in the\n                      <jats:italic toggle=\"yes\">PKD1<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">PKD2<\/jats:italic>\n                      genes account for 85 and 15% of cases of autosomal dominant polycystic kidney disease, respectively. Polycystin-2, the product of the\n                      <jats:italic toggle=\"yes\">PKD2<\/jats:italic>\n                      gene, is predicted to be an integral membrane protein with homology to a family of voltage-activated Ca\n                      <jats:sup>2+<\/jats:sup>\n                      channels.\n                      <jats:italic toggle=\"yes\">In vitro<\/jats:italic>\n                      studies suggest that it may interact with polycystin-1, the\n                      <jats:italic toggle=\"yes\">PKD1<\/jats:italic>\n                      gene product, via coiled-coil domains present in their C-terminal domains. In this study, the cellular and subcellular distribution of polycystin-2 is defined and compared with polycystin-1. A panel of rabbit polyclonal antisera was raised against polycystin-2 and shown to recognize a single band consistent with polycystin-2 in multiple tissues and cell lines by immunoprecipitation and Western blotting. Immunostaining of human and murine renal tissues demonstrated widespread and developmentally regulated expression of polycytin-2, with highest levels in the kidney in the thick ascending limbs of the loop of Henle and the distal convoluted tubule. In contrast, polycystin-1 expression, while localizing to the same tubular segments, was highest in the collecting ducts. Immunohistochemical staining and immunofluorescence microscopy localized polycystin-2 to the basolateral plasma membrane of kidney tubular epithelial cells compared with the junctional localization of polycystin-1. Differences in the developmental, cellular, and subcellular expression of polycystin-1 and polycystin-2 suggest that they may be able to function independently of each other in addition to a potential\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      interaction via their C-termini.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1681\/asn.v115814","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T18:15:44Z","timestamp":1620065744000},"page":"814-827","source":"Crossref","is-referenced-by-count":131,"title":["Cellular and Subcellular Distribution of Polycystin-2, the Protein Product of the PKD2 Gene"],"prefix":"10.1681","volume":"11","author":[{"given":"LUKAS","family":"FOGGENSTEINER","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. PAUL","family":"BEVAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RUTH","family":"THOMAS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NICHOLAS","family":"COLEMAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CATHERINE","family":"BOULTER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOHN","family":"BRADLEY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"OXANA","family":"IBRAGHIMOV-BESKROVNAYA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KATHERINE","family":"KLINGER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RICHARD","family":"SANDFORD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","container-title":["Journal of the American Society of Nephrology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/00001751-200005000-00002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T19:27:40Z","timestamp":1786130860000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00001751-200005000-00002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000]]}},"URL":"https:\/\/doi.org\/10.1681\/asn.v115814","relation":{},"ISSN":["1046-6673"],"issn-type":[{"value":"1046-6673","type":"print"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}